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Article

Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression

  • Authors:
    • Ning Liu
    • Ke Wei
    • Yu Xun
    • Xiaoxu Yang
    • Shiquan Gan
    • Hui Xiao
    • Ye Xiao
    • Feng Yan
    • Guie Xie
    • Tingting Wang
    • Yinke Yang
    • Jian Zhang
    • Xiang Hu
    • Shuanglin Xiang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Protein Chemistry and Developmental Biology of the Education Ministry of China, College of Life Science, Hunan Normal University, Changsha, Hunan 410081, P.R. China, Department of Molecular Medicine, College of Biology, Hunan University, Changsha, Hunan 410081, P.R. China
  • Pages: 7763-7769
    |
    Published online on: September 17, 2015
       https://doi.org/10.3892/mmr.2015.4336
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Abstract

Tumor necrosis factor alpha (TNFα)-induced protein 1 (TNFAIP1) was originally identified as a protein involved in DNA replication, DNA damage repair, apoptosis and the progression of certain diseases, such as Alzheimer's disease. In the present study, forskolin, a stimulant of cyclic adenosine monophosphate (cAMP), was found to significantly reduce human TNFAIP1 mRNA levels and TNFAIP1 promoter activity in the SKNSH human neuroblastoma cell line as indicated by polymerase chain reaction analysis and a luciferase reporter assay. The association between transcription factor cAMP response element‑binding protein (CREB) and TNFAIP1 was further investigated using loss- and gain of function-studies with western blot analysis and luciferase reporter assays. The CREB-specific inhibitor KG‑501 significantly increased TNFAIP1 protein levels, while overexpression of wild‑type CREB, but not CREB mutated at ser133a or its DNA-binding site, significantly decreased human TNFAIP1 protein levels and TNFAIP1 promoter activity in SKNSH cells. Furthermore, two CRE sites located at ‑285 and ‑425 bp of the human TNFAIP1 promoter were identified to be responsible for CREB‑induced inhibition of human TNFAIP1 promoter activity. Chromatin immunoprecipitation assays confirmed that CREB bound to the TNFAIP1 promoter region harboring these two CRE sites. A further luciferase reporter assay demonstrated that CREB phosphorylation on ser133 was responsible for forskolin‑induced inhibition of TNFAIP1 expression. In conclusion, the present study suggested that CREB is a negative regulator of the TNFAIP1 gene.
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1 

Wolf FW, Marks RM, Sarma V, Byers MG, Katz RW, Shows TB and Dixit VM: Characterization of a novel tumor necrosis factor-alpha-induced endothelial primary response gene. J Biol Chem. 267:1317–1326. 1992.PubMed/NCBI

2 

Zhou J, Hu X, Xiong X, Liu X, Liu Y, Ren K, Jiang T, Hu X and Zhang J: Cloning of two rat PDIP1 related genes and their interactions with proliferating cell nuclear antigen. J Exp Zool A Comp Exp Biol. 303:227–240. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Yang L, Liu N, Hu X, Zhang W, Wang T, Li H, Zhang B, Xiang S, Zhou J and Zhang J: CK2 phosphorylates TNFAIP1 to affect its subcellular localization and interaction with PCNA. Mol Biol Rep. 37:2967–2973. 2010. View Article : Google Scholar

4 

Kim DM, Chung KS, Choi SJ, Jung YJ, Park SK, Han GH, Ha JS, Song KB, Choi NS, Kim HM, et al: RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int J Cancer. 125:2520–2527. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Chen Y, Yang Z, Meng M, Zhao Y, Dong N, Yan H, Liu L, Ding M, Peng HB and Shao F: Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement. Mol Cell. 35:841–855. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Link CD, Taft A, Kapulkin V, Duke K, Kim S, Fei Q, Wood DE and Sahagan BG: Gene expression analysis in a transgenic Caenorhabditis elegans Alzheimer's disease model. Neurobiol Aging. 24:397–413. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Liu M, Sun Z, Zhou A, Li H, Yang L, Zhou C, Liu R, Hu X, Zhou J, Xiang S and Zhang J: Functional characterization of the promoter region of human TNFAIP1 gene. Mol Biol Rep. 37:1699–1705. 2010. View Article : Google Scholar

8 

Johannessen M, Delghandi MP, Seternes OM, Johansen B and Moens U: Synergistic activation of CREB-mediated transcription by forskolin and phorbol ester requires PKC and depends on the glutamine-rich Q2 transactivation domain. Cell Signal. 16:1187–1199. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Sárvári M, Szego EM, Barabás K, Jávor A, Tóth S, Kovács Z and Abrahám IM: Genistein induces phosphorylation of cAMP response element-binding protein in neonatal hypothalamus in vivo. J Neuroendocrinol. 21:1024–1028. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Best JL, Amezcua CA, Mayr B, Flechner L, Murawsky CM, Emerson B, Zor T, Gardner KH and Montminy M: Identification of small-molecule antagonists that inhibit an activator: Coactivator interaction. Proc Natl Acad Sci USA. 101:17622–17627. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Liu N, Yu Z, Li Y, Yuan J, Zhang J, Xiang S and Wang X: Transcriptional regulation of mouse neuroglobin gene by cyclic AMP responsive element binding protein (CREB) in N2a cells. Neurosci Lett. 534:333–337. 2013. View Article : Google Scholar :

12 

Liu N, Yu Z, Xiang S, Zhao S, Tjarnlund-Wolf A, Xing C, Zhang J and Wang X: Transcriptional regulation mechanisms of hypoxia-induced neuroglobin gene expression. Biochem J. 443:153–164. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Wang Z, Zhang XJ, Li T, Li J, Tang Y and Le W: Valproic acid reduces neuritic plaque formation and improves learning deficits in APP(Swe)/PS1(A246E) transgenic mice via preventing the prenatal hypoxia-induced down-regulation of neprilysin. CNS Neurosci Ther. 20:209–217. 2014. View Article : Google Scholar

14 

Li RP, Wang ZZ, Sun MX, Hou XL, Sun Y, Deng ZF and Xiao K: Polydatin protects learning and memory impairments in a rat model of vascular dementia. Phytomedicine. 19:677–681. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Vitolo OV, Sant'Angelo A, Costanzo V, Battaglia F, Arancio O and Shelanski M: Amyloid beta-peptide inhibition of the PKA/CREB pathway and long-term potentiation: Reversibility by drugs that enhance cAMP signaling. Proc Natl Acad Sci USA. 99:13217–13221. 2002. View Article : Google Scholar

16 

Bagheri M, Joghataei MT, Mohseni S and Roghani M: Genistein ameliorates learning and memory deficits in amyloid β(1–40) rat model of Alzheimer's disease. Neurobiol Learn Mem. 95:270–276. 2011. View Article : Google Scholar

17 

Gonzalez GA and Montminy MR: Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133. Cell. 59:675–680. 1989. View Article : Google Scholar : PubMed/NCBI

18 

Biermann J, Grieshaber P, Goebel U, Martin G, Thanos S, Di Giovanni S and Lagrèze WA: Valproic acid-mediated neuro-protection and regeneration in injured retinal ganglion cells. Invest Ophthalmol Vis Sci. 51:526–534. 2010. View Article : Google Scholar

19 

Lovekamp-Swan T, Glendenning M and Schreihofer DA: A high soy diet reduces programmed cell death and enhances bcl-xL expression in experimental stroke. Neuroscience. 148:644–652. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Liu H, Yang L, Zhao Y, Zeng G, Wu Y, Chen Y, Zhang J and Zeng Q: Estrogen is a novel regulator of Tnfaip1 in mouse hippocampus. Int J Mol Med. 34:219–227. 2014.PubMed/NCBI

21 

Walton M, Woodgate AM, Muravlev A, Xu R, During MJ and Dragunow M: CREB phosphorylation promotes nerve cell survival. J Neurochem. 73:1836–1842. 1999.PubMed/NCBI

22 

Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M and Matsumoto M: Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo. J Neurosci. 21:9204–9213. 2001.PubMed/NCBI

23 

Chrivia JC, Kwok RP, Lamb N, Hagiwara M, Montminy MR and Goodman RH: Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature. 365:855–859. 1993. View Article : Google Scholar : PubMed/NCBI

24 

Parker D, Ferreri K, Nakajima T, LaMorte VJ, Evans R, Koerber SC, Hoeger C and Montminy MR: Phosphorylation of CREB at Ser-133 induces complex formation with CREB-binding protein via a direct mechanism. Mol Cell Biol. 16:694–703. 1996. View Article : Google Scholar : PubMed/NCBI

25 

Wilson BE, Mochon E and Boxer LM: Induction of bcl-2 expression by phosphorylated CREB proteins during B-cell activation and rescue from apoptosis. Mol Cell Biol. 16:5546–5556. 1996. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu N, Wei K, Xun Y, Yang X, Gan S, Xiao H, Xiao Y, Yan F, Xie G, Wang T, Wang T, et al: Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression. Mol Med Rep 12: 7763-7769, 2015.
APA
Liu, N., Wei, K., Xun, Y., Yang, X., Gan, S., Xiao, H. ... Xiang, S. (2015). Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression. Molecular Medicine Reports, 12, 7763-7769. https://doi.org/10.3892/mmr.2015.4336
MLA
Liu, N., Wei, K., Xun, Y., Yang, X., Gan, S., Xiao, H., Xiao, Y., Yan, F., Xie, G., Wang, T., Yang, Y., Zhang, J., Hu, X., Xiang, S."Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression". Molecular Medicine Reports 12.5 (2015): 7763-7769.
Chicago
Liu, N., Wei, K., Xun, Y., Yang, X., Gan, S., Xiao, H., Xiao, Y., Yan, F., Xie, G., Wang, T., Yang, Y., Zhang, J., Hu, X., Xiang, S."Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression". Molecular Medicine Reports 12, no. 5 (2015): 7763-7769. https://doi.org/10.3892/mmr.2015.4336
Copy and paste a formatted citation
x
Spandidos Publications style
Liu N, Wei K, Xun Y, Yang X, Gan S, Xiao H, Xiao Y, Yan F, Xie G, Wang T, Wang T, et al: Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression. Mol Med Rep 12: 7763-7769, 2015.
APA
Liu, N., Wei, K., Xun, Y., Yang, X., Gan, S., Xiao, H. ... Xiang, S. (2015). Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression. Molecular Medicine Reports, 12, 7763-7769. https://doi.org/10.3892/mmr.2015.4336
MLA
Liu, N., Wei, K., Xun, Y., Yang, X., Gan, S., Xiao, H., Xiao, Y., Yan, F., Xie, G., Wang, T., Yang, Y., Zhang, J., Hu, X., Xiang, S."Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression". Molecular Medicine Reports 12.5 (2015): 7763-7769.
Chicago
Liu, N., Wei, K., Xun, Y., Yang, X., Gan, S., Xiao, H., Xiao, Y., Yan, F., Xie, G., Wang, T., Yang, Y., Zhang, J., Hu, X., Xiang, S."Transcription factor cyclic adenosine monophosphate responsive element binding protein negatively regulates tumor necrosis factor alpha-induced protein 1 expression". Molecular Medicine Reports 12, no. 5 (2015): 7763-7769. https://doi.org/10.3892/mmr.2015.4336
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