Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum

Liang,Zeng‑Enni; Yi,You‑Jin; Guo,Yu‑Tong; Wang,Ren‑Cai; Hu,Qiu‑Long; Xiong,Xing‑Yao

doi:10.3892/mmr.2015.4345

Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum

Authors:
- Zeng‑Enni Liang
- You‑Jin Yi
- Yu‑Tong Guo
- Ren‑Cai Wang
- Qiu‑Long Hu
- Xing‑Yao Xiong
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Affiliations: Hunan Agricultural Product Processing Institute, Changsha, Hunan 410128, P.R. China, College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China, State Key Laboratory of Sub‑Health Intervention Technology, State Administration of Traditional Chinese Medicine, Changsha, Hunan 410128, P.R. China, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China
Published online on: September 22, 2015 https://doi.org/10.3892/mmr.2015.4345
Pages: 7629-7636

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Abstract

Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAE‑cellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLP‑mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases‑3, ‑8 and ‑9 was involved in GLP‑stimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase‑3 proteins, whilst reducing the expression of cleaved poly(ADP‑ribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.

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