Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells

Wang,Penglong; Yuan,Xuan; Wang,Yan; Zhao,Hong; Sun,Xiling; Zheng,Qiusheng

doi:10.3892/mmr.2015.4346

Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells

Authors:
- Penglong Wang
- Xuan Yuan
- Yan Wang
- Hong Zhao
- Xiling Sun
- Qiusheng Zheng
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Affiliations: Key Laboratory of Xinjiang Endemic Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, Xinjiang 832002, P.R. China, Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Qingdao University Medical College, Qingdao, Shandong 266003, P.R. China, Shandong Provincial Key Laboratory of Heart‑Spleen Foundation of Traditional Chinese Medicine, Binzhou Medical College, Yantai, Shandong 264005, P.R. China
Published online on: September 22, 2015 https://doi.org/10.3892/mmr.2015.4346
Pages: 7623-7628

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Abstract

The current study investigated the mechanisms by which licochalcone C induces apoptosis of T24 human malignant bladder cancer cells. Cell viability was evaluated using an MTT assay. Apoptosis was investigated using a morphological assay, flow cytometry and a caspase‑3 activity assay. Alterations in the gene expression levels of Bcl‑2 family members were measured by semi‑quantitative reverse transcription‑polymerase chain reaction assays. The protein levels of pro‑caspase‑3 and cleaved poly(ADP ribose) polymerase were measured using western blotting. The results indicated that licochalcone C induced T24 cell apoptosis in a concentration‑dependent manner. Licochalcone C treatment reduced the levels of the anti‑apoptotic mRNAs (Bcl‑2, Bcl‑w and Bcl‑XL) and increased expression of the pro‑apoptotic mRNAs (Bax and Bim). The Bcl‑2 family inhibitor (ABT‑737) reduced apoptosis induced by licochalcone C in T24 cells. The current study demonstrated that licochalcone C may be a potential adjuvant therapeutic agent for bladder cancer.

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