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Article

Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells

  • Authors:
    • Penglong Wang
    • Xuan Yuan
    • Yan Wang
    • Hong Zhao
    • Xiling Sun
    • Qiusheng Zheng
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Xinjiang Endemic Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, Xinjiang 832002, P.R. China, Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Qingdao University Medical College, Qingdao, Shandong 266003, P.R. China, Shandong Provincial Key Laboratory of Heart‑Spleen Foundation of Traditional Chinese Medicine, Binzhou Medical College, Yantai, Shandong 264005, P.R. China
  • Pages: 7623-7628
    |
    Published online on: September 22, 2015
       https://doi.org/10.3892/mmr.2015.4346
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Abstract

The current study investigated the mechanisms by which licochalcone C induces apoptosis of T24 human malignant bladder cancer cells. Cell viability was evaluated using an MTT assay. Apoptosis was investigated using a morphological assay, flow cytometry and a caspase‑3 activity assay. Alterations in the gene expression levels of Bcl‑2 family members were measured by semi‑quantitative reverse transcription‑polymerase chain reaction assays. The protein levels of pro‑caspase‑3 and cleaved poly(ADP ribose) polymerase were measured using western blotting. The results indicated that licochalcone C induced T24 cell apoptosis in a concentration‑dependent manner. Licochalcone C treatment reduced the levels of the anti‑apoptotic mRNAs (Bcl‑2, Bcl‑w and Bcl‑XL) and increased expression of the pro‑apoptotic mRNAs (Bax and Bim). The Bcl‑2 family inhibitor (ABT‑737) reduced apoptosis induced by licochalcone C in T24 cells. The current study demonstrated that licochalcone C may be a potential adjuvant therapeutic agent for bladder cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang P, Yuan X, Wang Y, Zhao H, Sun X and Zheng Q: Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells. Mol Med Rep 12: 7623-7628, 2015.
APA
Wang, P., Yuan, X., Wang, Y., Zhao, H., Sun, X., & Zheng, Q. (2015). Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells. Molecular Medicine Reports, 12, 7623-7628. https://doi.org/10.3892/mmr.2015.4346
MLA
Wang, P., Yuan, X., Wang, Y., Zhao, H., Sun, X., Zheng, Q."Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells". Molecular Medicine Reports 12.5 (2015): 7623-7628.
Chicago
Wang, P., Yuan, X., Wang, Y., Zhao, H., Sun, X., Zheng, Q."Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells". Molecular Medicine Reports 12, no. 5 (2015): 7623-7628. https://doi.org/10.3892/mmr.2015.4346
Copy and paste a formatted citation
x
Spandidos Publications style
Wang P, Yuan X, Wang Y, Zhao H, Sun X and Zheng Q: Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells. Mol Med Rep 12: 7623-7628, 2015.
APA
Wang, P., Yuan, X., Wang, Y., Zhao, H., Sun, X., & Zheng, Q. (2015). Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells. Molecular Medicine Reports, 12, 7623-7628. https://doi.org/10.3892/mmr.2015.4346
MLA
Wang, P., Yuan, X., Wang, Y., Zhao, H., Sun, X., Zheng, Q."Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells". Molecular Medicine Reports 12.5 (2015): 7623-7628.
Chicago
Wang, P., Yuan, X., Wang, Y., Zhao, H., Sun, X., Zheng, Q."Licochalcone C induces apoptosis via B-cell lymphoma 2 family proteins in T24 cells". Molecular Medicine Reports 12, no. 5 (2015): 7623-7628. https://doi.org/10.3892/mmr.2015.4346
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