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Article

Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide

  • Authors:
    • Xiangyu Zhou
    • Xiaoyong Li
    • Meina Feng
    • Qi Zhang
    • Zhendong Yang
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The Affiliated First Hospital of Shizuishan, The Ningxia Medical University, Shizuishan, Ningxia 753200, P.R. China, Department of Pathology, Medical College, Hubei Polytechnic University, Huangshi, Hubei 435003, P.R. China, Department of Neurology, Wuhan Brain Hospital, Wuhan, Hubei 430010, P.R. China, Department of Neurology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430077, P.R. China, Department of Neurology, Wuhan No. 1 Hospital, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 7425-7432
    |
    Published online on: September 24, 2015
       https://doi.org/10.3892/mmr.2015.4358
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Abstract

The aim of the present study was to investigate the possible role of cytotoxic T lymphocytes (CTL) and mononuclear macrophages in the pathogenic processes of experimental animals. To construct a chronic experimental allergic encephalomyelitis (EAE) model, an artificially synthesized myelin oligodendrocyte glycoprotein (MOG)35‑55 peptide was used to induce C57BL/6 mice. Subsequently, the experimental animals were investigated at the level of their nervous function, and histopathological, immunohistochemical and fluorescence immunohistochemical experiments were performed at different time points following immunization. The expression of immune molecules and cytokines associated with the activation of the mononuclear macrophages and CTL during the different stages was assessed by western blotting and reverse transcription‑quantitative polymerase chain reaction. As a result, the MOG35‑55 peptide was identified as being successful at inducing C57BL/6 mice for the development of the EAE model. A modest level of mononuclear macrophage and lymphocyte infiltration was observed in the central nervous system (CNS), although no infiltration of neutrophils was observed. A sporadic flaky deletion of the myelin sheath was also identified. The activation and proliferation of mononuclear macrophages, including microglia cells, was clearly demonstrated. Furthermore, the expression levels of major histocompatibility complex class I and II molecules and interleukin‑12 in the brain, which is associated with the activation and proliferation of mononuclear macrophages, increased over the duration of the experiment compared with less pronounced changes in the expression levels of interferon (IFN)‑γ, Fas and perforin in the CNS, which are associated with the function of CTL. The secretion of IFN‑γ in the spleen increased during the morbidity peak, however, any noticeable activation and proliferation of CD8+ T cells was absent. These results demonstrated that the induced immune response mediated by mononuclear macrophages made a more important contribution compared with CTL towards the pathological process of myelin sheath injury. Mononuclear macrophages are therefore, identified as being one of the most significant effector cell types to directly injure the myelin sheath in the CNS.
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1 

Bradl M and Linington C: Animal model of demyelination. Brain Pathol. 6:303–311. 1996. View Article : Google Scholar : PubMed/NCBI

2 

Polman CH, Dijkstra CD, Sminia T and Koetsier JC: Immunohistological analysis of macrophages in the central nervous system of Lewis rats with experimental autoimmune encephalomyelitis. J Neuroimmunol. 11:215–21. 1986. View Article : Google Scholar : PubMed/NCBI

3 

Mendel 1, Keriero de Rosbo N and Ben-Nun A: A myelin oligodendrocyte glycoprotein peptide induces typical chronic experimental autoimmune encephalomyelitis in H-2b mice: Fine specificity and T cell receptor V beta expression of encephalitogenic T cells. Eur J Immunol. 25:1951–1959. 1995. View Article : Google Scholar : PubMed/NCBI

4 

Pál E, Yamamura T and Tabira T: Autonomic regulation of experimental autoimmune encephalomyelitis in IL-4 knockout mice. J Neuroimmunol. 100:149–155. 1999. View Article : Google Scholar

5 

Baumann N and Pham-Dinh D: Biology of oligodendrocyte and myelin in the mammalian central nervous system. Physiol Rev. 81:871–927. 2001.PubMed/NCBI

6 

Wang H, Munger KL, Reindl M, O'Reilly EJ, Levin LI, Berger T and Ascherio A: Myelin oligodendrocyte glycoprotein antibodies and multiple sclerosis in healthy young adults. Neurology. 71:1142–1146. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Roscoe WA, Welsh ME, Carter DE and Karlik SJ: VEGF and angiogenesis in acute and chronic MOG((35-55)) peptide induced EAE. J Neuroimmunol. 209:6–15. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Berard JL, Wolak K, Fournier S and David S: Characterization of relapsing-remitting and chronic forms of experimental autoimmune encephalomyelitis in C57BL/6 mice. Glia. 58:434–445. 2010.

9 

Slavin A, Ewing C, Liu J, Ichikawa M, Slavin J and Bernard CC: Induction of a multiple sclerosis-like disease in mice with an immunodominant epitope of myelin oligodendrocyte glycoprotein. Autoimmunity. 28:109–120. 1998. View Article : Google Scholar : PubMed/NCBI

10 

Kanamori M, Kawaguchi T, Nigro JM, Feuerstein BG, Berger MS, Miele L and Pieper RO: Contribution of Notch signaling activation to human glioblastoma multiforme. J Neurosurg. 106:417–427. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Rao P and Segal BM: Experimental autoimmune encephalomyelitis. Methods Mol Med. 102:363–375. 2004.PubMed/NCBI

12 

Steinman L: Antigen-specific therapy of multiple sclerosis: The long-sought magic bullet. Neurotherapeutics. 4:661–665. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Oukka M: Interplay between pathogenic Th17 and regulatory T cells. Ann Rheum Dis. 66(Suppl 3): iii87–iii90. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Edwards LJ, Robins RA and Constantinescu CS: Th17/Th1 phenotype in demyelinating disease. Cytokine. 50:19–23. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Benson JM, Stuckman SS, Cox KL, Wardrop RM, Gienapp IE, Cross AH, Trotter JL and Whitacre CC: Oral administration of myelin basic protein is superior to myelin in suppressing established relapsing experimental autoimmune encephalomyelitis. J Immunol. 162:6247541999.

16 

Qu N, Zhou XY, Han L, Wang L, Xu JX, Zhang T, Chu J, Chen Q, Wang JZ, Zhang Q and Tian Q: Combination of PPT with LiCl treatment prevented bilateral ovariectomy-induced hippocampal-dependent cognition deficit in rats. Mol Neurobiol. Dec 23;Epub ahead of print. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Lehuen A, Diana J, Zaccone P and Cooke A: Immune cell crosstalk in type 1 diabetes. Nat Rev Immunol. 10:501–513. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Lisak RP: Neurodegeneration in multiple sclerosis: Defining the problem. Neurology. 68:S5–S12; discussion S43-S54. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Goverman J: Autoimmune T cell responses in the central nervous system. Nat Rev Immunol. 9:393–407. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Miller AH, Maletic V and Raison CL: Inflammation and its discontents: The role of cytokines in the pathophysiology of major depression. Biol Psychiatry. 65:732–741. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Murphy AC, Lalor SJ, Lynch MA and Mills KH: Infiltration of Th1 and Th17 cells and activation of microglia in the CNS during the course of experimental autoimmune encephalomyelitis. Brain Behav Immun. 24:641–651. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Friese MA and Fugger L: Pathogenic CD8(+) T cells in multiple sclerosis. Ann Neurol. 66:132–141. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Fletcher JM, Lalor SJ, Sweeney CM, Tubridy N and Mills KH: T cells in multiple sclerosis and experimental autoimmune encephalomyelitis. Clin Exp Immunol. 162:1–11. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Saxena A, Martin-Blondel G, Mars LT and Liblau RS: Role of CD8 T cell subsets in the pathogenesis of multiple sclerosis. FEBS Lett. 585:3758–3763. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Sobottka B, Harrer MD, Ziegler U, Fischer K, Wiendl H, Hünig T, Becher B and Goebels N: Collateral bystander damage by myelin-directed CD8+ T cells causes axonal loss. Am J Pathol. 175:1160–1166. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Niederkorn JY: Emerging concepts in CD8(+) T regulatory cells. Curr Opin Immunol. 20:327–331. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou X, Li X, Feng M, Zhang Q and Yang Z: Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide. Mol Med Rep 12: 7425-7432, 2015.
APA
Zhou, X., Li, X., Feng, M., Zhang, Q., & Yang, Z. (2015). Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide. Molecular Medicine Reports, 12, 7425-7432. https://doi.org/10.3892/mmr.2015.4358
MLA
Zhou, X., Li, X., Feng, M., Zhang, Q., Yang, Z."Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide". Molecular Medicine Reports 12.5 (2015): 7425-7432.
Chicago
Zhou, X., Li, X., Feng, M., Zhang, Q., Yang, Z."Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide". Molecular Medicine Reports 12, no. 5 (2015): 7425-7432. https://doi.org/10.3892/mmr.2015.4358
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou X, Li X, Feng M, Zhang Q and Yang Z: Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide. Mol Med Rep 12: 7425-7432, 2015.
APA
Zhou, X., Li, X., Feng, M., Zhang, Q., & Yang, Z. (2015). Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide. Molecular Medicine Reports, 12, 7425-7432. https://doi.org/10.3892/mmr.2015.4358
MLA
Zhou, X., Li, X., Feng, M., Zhang, Q., Yang, Z."Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide". Molecular Medicine Reports 12.5 (2015): 7425-7432.
Chicago
Zhou, X., Li, X., Feng, M., Zhang, Q., Yang, Z."Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide". Molecular Medicine Reports 12, no. 5 (2015): 7425-7432. https://doi.org/10.3892/mmr.2015.4358
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