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Article

Gene expression profiling of CD133-positive cells in coronary artery disease

  • Authors:
    • Jiayu Li
    • Changyu Zhou
    • Jiarui Li
    • Yingchun Wan
    • Tao Li
    • Piyong Ma
    • Yingjian Wang
    • Haiyan Sang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Gastroenterology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Pharmacy, The Tumor Hospital of Jilin, Changchun, Jilin 130033, P.R. China, Department of Endocrinology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Emergency, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Gynaecology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
  • Pages: 7512-7516
    |
    Published online on: September 24, 2015
       https://doi.org/10.3892/mmr.2015.4359
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Abstract

Gene expression profiles of CD133-positive cells from patients with coronary artery disease (CAD) were analyzed to identify key genes associated with cardiac therapy. Furthermore, the effect of exercise on gene expression was also investigated. Gene expression data set (accession number: GSE18608) was downloaded from the Gene Expression Omnibus, including blood samples from four healthy subjects (H), and from 10 patients with coronary artery disease at baseline (B) and after 3 months (3M) of exercise. Differential analysis was performed for H vs. B and H vs. 3M using limma package of R. Two‑way cluster analysis was performed using the expression levels of the differentially expressed genes (DEGs) by package pheatmap of R. Functional enrichment analysis was applied on the DEGs using the Database for Annotation, Visualization and Integrated Discovery. Relevant small molecules were predicted using the Connectivity map database (cMap). A total of 131 and 71 DEGs were identified in patients with CAD prior to and following 3 months of exercise. The two groups of DEGs were compared and 44 genes overlapped. In cluster analysis with the expression levels of the common DEGs, patients with CAD could be well separated from the healthy controls. Functional enrichment analysis showed that response to peptide hormone stimulus and anti‑apoptosis pathways were significantly enriched in the common DEGs. A total of 12 relevant small molecules were revealed by cMap based upon the expression levels of common DEGs, such as 5252917 and MG‑262. Three months of exercise in part normalized the gene expression in CAD patients. The genes not altered by exercise may be the targets of small molecules, such as 5252917 and MG-262.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Zhou C, Li J, Wan Y, Li T, Ma P, Wang Y and Sang H: Gene expression profiling of CD133-positive cells in coronary artery disease. Mol Med Rep 12: 7512-7516, 2015.
APA
Li, J., Zhou, C., Li, J., Wan, Y., Li, T., Ma, P. ... Sang, H. (2015). Gene expression profiling of CD133-positive cells in coronary artery disease. Molecular Medicine Reports, 12, 7512-7516. https://doi.org/10.3892/mmr.2015.4359
MLA
Li, J., Zhou, C., Li, J., Wan, Y., Li, T., Ma, P., Wang, Y., Sang, H."Gene expression profiling of CD133-positive cells in coronary artery disease". Molecular Medicine Reports 12.5 (2015): 7512-7516.
Chicago
Li, J., Zhou, C., Li, J., Wan, Y., Li, T., Ma, P., Wang, Y., Sang, H."Gene expression profiling of CD133-positive cells in coronary artery disease". Molecular Medicine Reports 12, no. 5 (2015): 7512-7516. https://doi.org/10.3892/mmr.2015.4359
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Zhou C, Li J, Wan Y, Li T, Ma P, Wang Y and Sang H: Gene expression profiling of CD133-positive cells in coronary artery disease. Mol Med Rep 12: 7512-7516, 2015.
APA
Li, J., Zhou, C., Li, J., Wan, Y., Li, T., Ma, P. ... Sang, H. (2015). Gene expression profiling of CD133-positive cells in coronary artery disease. Molecular Medicine Reports, 12, 7512-7516. https://doi.org/10.3892/mmr.2015.4359
MLA
Li, J., Zhou, C., Li, J., Wan, Y., Li, T., Ma, P., Wang, Y., Sang, H."Gene expression profiling of CD133-positive cells in coronary artery disease". Molecular Medicine Reports 12.5 (2015): 7512-7516.
Chicago
Li, J., Zhou, C., Li, J., Wan, Y., Li, T., Ma, P., Wang, Y., Sang, H."Gene expression profiling of CD133-positive cells in coronary artery disease". Molecular Medicine Reports 12, no. 5 (2015): 7512-7516. https://doi.org/10.3892/mmr.2015.4359
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