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Article

Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats

  • Authors:
    • Jian Gu
    • Wei Gu
    • Chuanming Lin
    • Hao Gu
    • Wei Wu
    • Jie Yin
    • Jun Ni
    • Xiaoping Pei
    • Mei Sun
    • Fangfang Wang
    • Zou Li
    • Xinzheng Cai
    • Minmin Ren
    • Zhang Yu
    • Xiang Gu
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China, Jiangsu Province Brain Hospital, Nanjing, Jiangsu 210000, P.R. China, Department of Cardiology, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China
  • Pages: 7463-7470
    |
    Published online on: September 30, 2015
       https://doi.org/10.3892/mmr.2015.4394
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Abstract

Human umbilical cord mesenchymal stem cells (hUC‑MSCs) hold great potential in the search for therapies to treat refractory diseases, including rheumatoid arthritis (RA), due to their potential regenerative ability and extensive source. However, the role of hUC‑MSCs in vivo and the repair mechanisms for RA remain to be fully elucidated. The present study aimed to determine whether hUC‑MSCs exert immunomodulatory effects and have anti‑inflammatory capabilities in the treatment of embolisms. Following the transplantation of hUC‑MSCs into collagen type Ⅱ‑induced arthritic (CIA) model rats, magnetic resonance imaging (MRI) in vivo was performed, and the levels of interleukin (IL)‑1, IL‑17, tumor necrosis factor (TNF)‑α, vascular endothelial growth factor (VEGF), tissue factor (TF), CD4+CD25+ T cells (Treg) and antithrombin (AT) were measured. Bromodeoxyuridine staining was performed for histopathological examinations. As revealed by immunofluorescence and MRI experiments, the injected hUC‑MSCs preferentially migrated to the inflammatory joint sites of the rats. The Treg cell percentage and AT levels in the hUC‑MSC‑treated group were markedly increased, whereas the levels of IL‑1, IL‑17, TNF‑α, VEGF and TF were decreased compared with those in the CIA model group. The values determined for these parameters in the hUC‑MSC‑treated group returned to approximately the identical values as those of the control group on day 35 post‑therapy. Superparamagnetic iron oxide nanoparticles (SPIONs) may serve as an effective, non‑invasive method for tracking transplanted cells in vivo. The present study provided direct evidence that hUC‑MSCs in the CIA rat model migrated to the inflammatory joint sites, effectively promoting recovery from collagen type II damage and thereby improving the immune‑associated prothrombotic state.
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View References

1 

Lee DM and Weinblatt ME: Rheumatoid arthritis. Lancet. 358:903–911. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Choy E: Understanding the dynamics: Pathways involved in the pathogenesis of rheumatoid arthritis. Rheumatology (Oxford). 51(Suppl 5): v3–v11. 2012. View Article : Google Scholar

3 

Rampersad RR, Tarrant TK, Vallanat CT, Quintero-Matthews T, Weeks MF, Esserman DA, Clark J, Di Padova F, Patel DD, Fong AM and Liu P: Enhanced Th17-cell responses render CCR2-deficient mice more susceptible for autoimmune arthritis. PLoS One. 6:e258332011. View Article : Google Scholar : PubMed/NCBI

4 

Aksu K, Donmez A and Keser G: Inflammation-induced thrombosis: Mechanisms, disease associations and management. Curr Pharm Des. 18:1478–1493. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Boilard E, Nigrovic PA, Larabee K, Watts GF, Coblyn JS, Weinblatt ME, Massarotti EM, Remold-O'Donnell E, Farndale RW, Ware J and Lee DM: Platelets amplify inflammation in arthritis via collagen-dependent microparticle production. Science. 327:580–583. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Snowden JA, Saccardi R, Allez M, Ardizzone S, Arnold R, Cervera R, Denton C, Hawkey C, Labopin M, Mancardi G, et al: Haematopoietic SCT in severe autoimmune diseases: Updated guidelines of the European group for blood and marrow transplantation. Bone Marrow Transplant. 47:770–790. 2012. View Article : Google Scholar :

7 

Liu R, Zhang Z, Lu Z, Borlongan C, Pan J, Chen J, Qian L, Liu Z, Zhu L, Zhang J and Xu Y: Human umbilical cord stem cells ameliorate experimental autoimmune encephalomyelitis by regulating immunoinflammation and remyelination. Stem Cells Dev. 22:1053–1062. 2013. View Article : Google Scholar

8 

Chao KC, Chao KF, Fu YS and Liu SH: Islet-like clusters derived from mesenchymal stem cells in Wharton's Jelly of the human umbilical cord for transplantation to control type 1 diabetes. PLoS One. 3:e14512008. View Article : Google Scholar : PubMed/NCBI

9 

Van de Putte LB, Tyndall A, van den Hoogen FH and Smolen JS: Hematopoietic stem cell transplants for autoimmune disease: Role of EULAR. European League Against Rheumatism. J Rheumatol Suppl. 48:98–99. 1997.PubMed/NCBI

10 

Xie J, Liu G, Eden HS, Ai H and Chen X: Surface-engineered magnetic nanoparticle platforms for cancer imaging and therapy. Acc Chem Res. 44:883–892. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Lin CM, Gu J, Zhang Y, Shen LJ, Ma L, Ni J, Wang ZQ and Wu W: Effect of UC-MSCs on inflammation and thrombosis of the rats with collagen type II induced arthritis. Zhonghua Xue Ye Xue Za Zhi. 33:215–219. 2012.In Chinese. PubMed/NCBI

12 

Lange F, Bajtner E, Rintisch C, Nandakumar KS, Sack U and Holmdahl R: Methotrexate ameliorates T cell dependent autoimmune arthritis and encephalomyelitis but not antibody induced or fibroblast induced arthritis. Ann Rheum Dis. 64:599–605. 2005. View Article : Google Scholar

13 

Mahmoudi M, Sant S, Wang B, Laurent S and Sen T: Superparamagnetic iron oxide nanoparticles (SPIONs): Development, surface modification and applications in chemotherapy. Adv Drug Deliv Rev. 63:24–46. 2011. View Article : Google Scholar

14 

Lefevre S, Ruimy D, Jehl F, Neuville A, Robert P, Sordet C, Ehlinger M, Dietemann JL and Bierry G: Septic arthritis: Monitoring with USPIO enhanced macrophage MR imaging. Radiology. 258:722–728. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Hu SL, Zhang JQ, Hu X, Hu R, Luo HS, Li F, Xia YZ, Li JT, Lin JK, Zhu G and Feng H: In vitro labeling of human umbilical cord mesenchymal stem cells with superparamagnetic iron oxide nanoparticles. J Cell Biochem. 108:529–535. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Janic B, Rad AM, Jordan EK, Iskander AS, Ali MM, Varma NR, Frank JA and Arbab AS: Optimization and validation of FePro cell labeling method. PLoS One. 4:e58732009. View Article : Google Scholar : PubMed/NCBI

17 

De Almeida DE, Ling S, Pi X, Hartmann-Scruggs AM, Pumpens P and Holoshitz J: Immune dysregulation by the rheumatoid arthritis shared epitope. J Immunol. 185:1927–1934. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Liu X, Ye F, Xiong H, Hu DN, et al: IL-1β induces IL-6 production in retinal Müller cells predominantly through the activation of p38 MAPK/NF-κB signaling pathway. Exp Cell Res. 331:223–231. 2015. View Article : Google Scholar

19 

Joseph L, Fink LM and Hauer-Jensen M: Cytokines in coagulation and thrombosis: A preclinical and clinical review. Blood Coagul Fibrinolysis. 13:105–116. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Kouri VP, Olkkonen J, Ainola M, Li TF, Björkman L, Konttinen YT and Mandelin J: Neutrophils produce interleukin-17B in rheumatoid synovial tissue. Rheumatology (Oxford). 53:39–47. 2014. View Article : Google Scholar

21 

Noma H, Mimura T and Eguchi S: Association of inflammatory factors with macular edema in branch retinal vein occlusion. JAMA Ophthalmol. 131:160–165. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Undas A, Gissel M, Kwasny-Krochin B, Gluszko P, Mann KG and Brummel-Ziedins KE: Thrombin generation in rheumatoid arthritis: Dependence on plasma factor composition. Thromb Haemost. 104:224–230. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Sun L, Akiyama K, Zhang H, Hou Y, Zhao S, Xu T, Le A and Shi S: Mesenchymal stem cell transplantation reverses multiorgan dysfunction in systemic lupus erythematosus mice and humans. Stem Cells. 27:1421–1432. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Gu ZF, Akiyama K, Ma X, Zhang H, Feng X, Yao G, Hou Y, Lu L, Gilkeson GS, Silver RM, et al: Transplantation of umbilical cord mesenchymal stem cells alleviates lupus nephritis in MRL/lpr mice. Lupus. 19:1502–1514. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gu J, Gu W, Lin C, Gu H, Wu W, Yin J, Ni J, Pei X, Sun M, Wang F, Wang F, et al: Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats. Mol Med Rep 12: 7463-7470, 2015.
APA
Gu, J., Gu, W., Lin, C., Gu, H., Wu, W., Yin, J. ... Gu, X. (2015). Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats. Molecular Medicine Reports, 12, 7463-7470. https://doi.org/10.3892/mmr.2015.4394
MLA
Gu, J., Gu, W., Lin, C., Gu, H., Wu, W., Yin, J., Ni, J., Pei, X., Sun, M., Wang, F., Li, Z., Cai, X., Ren, M., Yu, Z., Gu, X."Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats". Molecular Medicine Reports 12.5 (2015): 7463-7470.
Chicago
Gu, J., Gu, W., Lin, C., Gu, H., Wu, W., Yin, J., Ni, J., Pei, X., Sun, M., Wang, F., Li, Z., Cai, X., Ren, M., Yu, Z., Gu, X."Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats". Molecular Medicine Reports 12, no. 5 (2015): 7463-7470. https://doi.org/10.3892/mmr.2015.4394
Copy and paste a formatted citation
x
Spandidos Publications style
Gu J, Gu W, Lin C, Gu H, Wu W, Yin J, Ni J, Pei X, Sun M, Wang F, Wang F, et al: Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats. Mol Med Rep 12: 7463-7470, 2015.
APA
Gu, J., Gu, W., Lin, C., Gu, H., Wu, W., Yin, J. ... Gu, X. (2015). Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats. Molecular Medicine Reports, 12, 7463-7470. https://doi.org/10.3892/mmr.2015.4394
MLA
Gu, J., Gu, W., Lin, C., Gu, H., Wu, W., Yin, J., Ni, J., Pei, X., Sun, M., Wang, F., Li, Z., Cai, X., Ren, M., Yu, Z., Gu, X."Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats". Molecular Medicine Reports 12.5 (2015): 7463-7470.
Chicago
Gu, J., Gu, W., Lin, C., Gu, H., Wu, W., Yin, J., Ni, J., Pei, X., Sun, M., Wang, F., Li, Z., Cai, X., Ren, M., Yu, Z., Gu, X."Human umbilical cord mesenchymal stem cells improve the immune‑associated inflammatory and prothrombotic state in collagen type‑Ⅱ‑induced arthritic rats". Molecular Medicine Reports 12, no. 5 (2015): 7463-7470. https://doi.org/10.3892/mmr.2015.4394
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