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Article

Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells

Corrigendum in: /10.3892/mmr.2021.11920
  • Authors:
    • Xiuying Li
    • Qiwei Yang
    • Jinping Bai
    • Yali Xuan
    • Yimin Wang
  • View Affiliations / Copyright

    Affiliations: The Central Laboratory, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China, Department of Pathology, The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun, Jilin 130021, P.R. China, Princess Margaret Hospital, Toronto, ON M5G2M9, Canada
  • Pages: 7721-7727
    |
    Published online on: September 30, 2015
       https://doi.org/10.3892/mmr.2015.4396
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Abstract

Accurate gene expression analysis relies on the selection of a stable reference gene, as unstable reference genes can alter experimental results and conclusions. It is widely‑accepted that reference genes exhibit different expression levels in different types of tissues and cells. Therefore, it is essential to screen for stably‑expressed reference genes in the cells and tissues used for experimental analysis prior to performing reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). In the present study, eight reference genes were screened for their suitability for RT‑qPCR in five T lymphocytes co‑cultured with mesenchymal stem cells from different sources. Using NormFinder, geNorm, and BestKeeper algorithms consistently demonstrated that RPL13A and B2M were the optimal reference genes for the normalization of RT‑qPCR data obtained from T lymphocytes, whereas glyceraldehyde 3‑phosphate dehydrogenase was not a suitable reference gene due to its extensive variability in expression. These findings highlight the importance of evaluating reference genes for RT‑qPCR.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Yang Q, Bai J, Xuan Y and Wang Y: Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells Corrigendum in /10.3892/mmr.2021.11920. Mol Med Rep 12: 7721-7727, 2015.
APA
Li, X., Yang, Q., Bai, J., Xuan, Y., & Wang, Y. (2015). Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells Corrigendum in /10.3892/mmr.2021.11920. Molecular Medicine Reports, 12, 7721-7727. https://doi.org/10.3892/mmr.2015.4396
MLA
Li, X., Yang, Q., Bai, J., Xuan, Y., Wang, Y."Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells Corrigendum in /10.3892/mmr.2021.11920". Molecular Medicine Reports 12.5 (2015): 7721-7727.
Chicago
Li, X., Yang, Q., Bai, J., Xuan, Y., Wang, Y."Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells Corrigendum in /10.3892/mmr.2021.11920". Molecular Medicine Reports 12, no. 5 (2015): 7721-7727. https://doi.org/10.3892/mmr.2015.4396
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Yang Q, Bai J, Xuan Y and Wang Y: Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells Corrigendum in /10.3892/mmr.2021.11920. Mol Med Rep 12: 7721-7727, 2015.
APA
Li, X., Yang, Q., Bai, J., Xuan, Y., & Wang, Y. (2015). Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells Corrigendum in /10.3892/mmr.2021.11920. Molecular Medicine Reports, 12, 7721-7727. https://doi.org/10.3892/mmr.2015.4396
MLA
Li, X., Yang, Q., Bai, J., Xuan, Y., Wang, Y."Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells Corrigendum in /10.3892/mmr.2021.11920". Molecular Medicine Reports 12.5 (2015): 7721-7727.
Chicago
Li, X., Yang, Q., Bai, J., Xuan, Y., Wang, Y."Evaluation of eight reference genes for quantitative polymerase chain reaction analysis in human T lymphocytes co‑cultured with mesenchymal stem cells Corrigendum in /10.3892/mmr.2021.11920". Molecular Medicine Reports 12, no. 5 (2015): 7721-7727. https://doi.org/10.3892/mmr.2015.4396
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