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Article

Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells

  • Authors:
    • Lei Jiang
    • Jiaming Wen
    • Wei Luo
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, The Fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, P.R. China, Department of Urology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China
  • Pages: 7526-7530
    |
    Published online on: September 30, 2015
       https://doi.org/10.3892/mmr.2015.4404
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Abstract

The serine/threonine kinases, Rho‑associated protein kinase I and II (ROCK I and II), regulate the cytoskeleton by acting downstream of the small GTPase, Rho, and have been implicated in tumorigenesis and cancer metastasis. Inhibition of ROCK signaling has been shown to suppress the invasion and migration of several types of cancer cells. In this study, the effect of the ROCK inhibitor, Y‑27632, on the proliferation and invasion of T24 and 5637 bladder cancer cells was investigated. In the proliferation assays, the cells were exposed to 0, 10, 25, 50, 75, 100, 125 or 150 µmol/l Y‑27632 and proliferation was determined using Cell Counting kit‑8 after 24, 48 and 72 h. In the invasion assays, the cells were placed in the upper chamber of transwell plates and subjected to 0, 25, 50 or 75 µmol/l Y‑27632 for 24 h, after which invasion was measured. Y‑27632 significantly suppressed the cell proliferation of T24 and 5637 cells in a concentration- and time‑dependent manner. Y‑27632 also inhibited the invasion of T24 and 5637 cells in a concentration‑dependent manner (P<0.001). In addition, Y‑27632 suppressed myosin light chain kinase (MLCK) phosphorylation in T24 and 5637 cells, confirming that it is also a downstream effector of the Rho/ROCK pathway in T24 and 5637 bladder cancer cells. In conclusion, the Rho/ROCK/P‑MLCK pathway may be important in tumor cell metastasis in bladder cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang L, Wen J and Luo W: Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells. Mol Med Rep 12: 7526-7530, 2015.
APA
Jiang, L., Wen, J., & Luo, W. (2015). Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells. Molecular Medicine Reports, 12, 7526-7530. https://doi.org/10.3892/mmr.2015.4404
MLA
Jiang, L., Wen, J., Luo, W."Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells". Molecular Medicine Reports 12.5 (2015): 7526-7530.
Chicago
Jiang, L., Wen, J., Luo, W."Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells". Molecular Medicine Reports 12, no. 5 (2015): 7526-7530. https://doi.org/10.3892/mmr.2015.4404
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang L, Wen J and Luo W: Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells. Mol Med Rep 12: 7526-7530, 2015.
APA
Jiang, L., Wen, J., & Luo, W. (2015). Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells. Molecular Medicine Reports, 12, 7526-7530. https://doi.org/10.3892/mmr.2015.4404
MLA
Jiang, L., Wen, J., Luo, W."Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells". Molecular Medicine Reports 12.5 (2015): 7526-7530.
Chicago
Jiang, L., Wen, J., Luo, W."Rho‑associated kinase inhibitor, Y‑27632, inhibits the invasion and proliferation of T24 and 5367 bladder cancer cells". Molecular Medicine Reports 12, no. 5 (2015): 7526-7530. https://doi.org/10.3892/mmr.2015.4404
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