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Article

Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer

  • Authors:
    • Xiaopeng Wu
    • Bin Qiao
    • Qin Liu
    • Weiguo Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China
  • Pages: 7419-7424
    |
    Published online on: October 1, 2015
       https://doi.org/10.3892/mmr.2015.4410
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Abstract

Extracellular matrix metalloproteinase inducer (EMMPRIN) exerts important roles in tumor progression, including angiogenesis, metastasis and therapy resistance. The epithelial‑mesenchymal transition (EMT), which is induced by hypoxia, is an important process in cancer metastasis. However, the association between hypoxia and EMMPRIN remains to be elucidated in esophageal cancer. The expression of EMMPRIN was determined by western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), and EMT markers were analyzed by western blotting, RT‑qPCR and immunofluoresence. The migration and invasion of cells was investigated by Transwell assay. The results indicated that the expression levels of EMMPRIN in esophageal cancer cells were markedly higher compared with those in normal esophageal cells. EMMPRIN was able to promote esophageal cancer cell migration and invasion under both hypoxic or normoxic conditions, as demonstrated by the migration and invasion assay. The expression levels of E‑cadherin were reduced, and those of snail family zinc finger 1, fibronectin, α‑smooth muscle actin and fibroblast secretory protein 1 increased in esophageal cancer cells following treatment with human recombinant EMMPRIN under hypoxic conditions. The mRNA expression levels of the EMT markers were similar to those of the protein expression levels. Furthermore, the results demonstrated that EMMPRIN was regulated by hypoxia‑inducible factor (HIF)‑1α. These data suggested that EMMPRIN promoted metastasis and the EMT in esophageal cancer cells by regulating HIF-1α.
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1 

Weidle UH, Scheuer W, Eggle D, Klostermann S and Stockinger H: Cancer-related issues of CD147. Cancer Genomics Proteomics. 7:157–69. 2010.PubMed/NCBI

2 

Hao JL, Cozzi PJ, Khatri A, Power CA and Li Y: CD147/EMMPRIN and CD44 are potential therapeutic targets for metastatic prostate cancer. Curr Cancer Drug Targets. 10:287–306. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Kanekura T and Chen X: CD147/basigin promotes progression of malignant melanoma and other cancers. J Dermatol Sci. 57:149–54. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Toole BP and Slomiany MG: Hyaluronan, CD44 and Emmprin: Partners in cancer cell chemoresistance. Drug Resist Updat. 11:110–21. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Iacono KT, Brown AL, Greene MI and Saouaf SJ: CD147 immunoglobulin superfamily receptor function and role in pathology. Exp Mol Pathol. 83:283–95. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Nabeshima K, Iwasaki H, Koga K, Hojo H, Suzumiya J and Kikuchi M: Emmprin (basigin/CD147): Matrix metalloproteinase modulator and multifunctional cell recognition molecule that plays a critical role in cancer progression. Pathol Int. 56:359–367. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Yurchenko V, Constant S and Bukrinsky M: Dealing with the family: CD147 interactions with cyclophilins. Immunology. 117:301–309. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Yan L, Zucker S and Toole BP: Roles of the multifunctional glycoprotein, emmprin (basigin; CD147), in tumour progression. Thromb Haemost. 93:199–204. 2005.PubMed/NCBI

9 

Muramatsu T and Miyauchi T: Basigin (CD147): A multi-functional transmembrane protein involved in reproduction, neural function, inflammation and tumor invasion. Histol Histopathol. 18:981–987. 2003.PubMed/NCBI

10 

Szubert S, Szpurek D, Moszynski R, Nowicki M, Frankowski A, Sajdak S and Michalak S: Extracellular matrix metalloproteinase inducer (EMMPRIN) expression correlates positively with active angiogenesis and negatively with basic fibroblast growth factor expression in epithelial ovarian cancer. J Cancer Res Clin Oncol. 140:361–369. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Redzic JS, Kendrick AA, Bahmed K, Dahl KD, Pearson CG, Robinson WA, Robinson SE, Graner MW and Eisenmesser EZ: Extracellular vesicles secreted from cancer cell lines stimulate secretion of MMP-9, IL-6, TGF-β1 and EMMPRIN. PLoS One. 8:e712252013. View Article : Google Scholar

12 

Grass GD, Tolliver LB, Bratoeva M and Toole BP: CD147, CD44, and the epidermal growth factor receptor (EGFR) signaling pathway cooperate to regulate breast epithelial cell invasiveness. J Biol Chem. 288:26089–26104. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Papadimitropoulou A and Mamalaki A: The glycosylated IgII extracellular domain of EMMPRIN is implicated in the induction of MMP-2. Mol Cell Biochem. 379:107–113. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Zhao SH, Wang Y, Wen L, Zhai ZB, Ai ZH, Yao NL, Wang L, Liu WC, Chen BL, Li Y, et al: Basigin-2 is the predominant basigin isoform that promotes tumor cell migration and invasion and correlates with poor prognosis in epithelial ovarian cancer. J Transl Med. 11:92–101. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Kang MJ, Kim HP, Lee KS, Yoo YD, Kwon YT, Kim KM, Kim TY and Yi EC: Proteomic analysis reveals that CD147/EMMPRIN confers chemoresistance in cancer stem cell-like cells. Proteomics. 13:1714–1725. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Zhu S, Chu D, Zhang Y, Wang X, Gong L, Han X, Yao L, Lan M, Li Y and Zhang W: EMMPRIN/CD147 expression is associated with disease-free survival of patients with colorectal cancer. Med Oncol. 30:3692013. View Article : Google Scholar : PubMed/NCBI

17 

Dai L, Guinea MC, Slomiany MG, Bratoeva M, Grass GD, Tolliver LB, Maria BL and Toole BP: CD147-dependent heterogeneity in malignant and chemoresistant properties of cancer cells. Am J Pathol. 182:577–585. 2013. View Article : Google Scholar :

18 

Hibino T, Sakaguchi M, Miyamoto S, Yamamoto M, Motoyama A, Hosoi J, Shimokata T, Ito T, Tsuboi R and Huh NH: S100A9 is a novel ligand of EMMPRIN that promotes melanoma metastasis. Cancer Res. 73:172–183. 2013. View Article : Google Scholar

19 

Ke X, Fei F, Chen Y, Xu L, Zhang Z, Huang Q, Zhang H, Yang H, Chen Z and Xing J: Hypoxia upregulates CD147 through a combined effect of HIF-1α and Sp1 to promote glycolysis and tumor progression in epithelial solid tumors. Carcinogenesis. 33:1598–1607. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Sweeny L, Liu Z, Bush BD, Hartman Y, Zhou T and Rosenthal EL: CD147 and AGR2 expression promote cellular proliferation and metastasis of head and neck squamous cell carcinoma. Exp Cell Res. 318:1788–1798. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Nakamura K, Kodama J, Hongo A and Hiramatsu Y: Role of emmprin in endometrial cancer. BMC Cancer. 12:191–201. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Grass GD, Bratoeva M and Toole BP: Regulation of invadopodia formation and activity by CD147. J Cell Sci. 125:777–788. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Cui HY, Guo T, Wang SJ, Zhao P, Dong ZS, Zhang Y, Jiang JL, Chen ZN and Yu XL: Dimerization is essential for HAb18G/CD147 promoting tumor invasion via MAPK pathway. Biochem Biophys Res Commun. 419:517–522. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Han M, Trotta P, Coleman C and Linask KK: MCT-4, A511/Basigin and EF5 expression patterns during early chick cardiomyogenesis indicate cardiac cell differentiation occurs in a hypoxic environment. Dev Dyn. 235:124–131. 2006. View Article : Google Scholar

25 

Boulos S, Meloni BP, Arthur PG, Majda B, Bojarski C and Knuckey NW: Evidence that intracellular cyclophilin A and cyclophilin A/CD147 receptor-mediated ERK1/2 signalling can protect neurons against in vitro oxidative and ischemic injury. Neurobiol Dis. 25:54–64. 2007. View Article : Google Scholar

26 

Philip B, Ito K, Moreno-Sánchez R and Ralph SJ: HIF expression and the role of hypoxic microenvironments within primary tumours as protective sites driving cancer stem cell renewal and metastatic progression. Carcinogenesis. 34:1699–1707. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Tsai YP and Wu KJ: Hypoxia-regulated target genes implicated in tumor metastasis. J Biomed Sci. 19:1022012. View Article : Google Scholar : PubMed/NCBI

28 

Le Floch R, Chiche J, Marchiq I, Naiken T, Ilc K, Murray CM, Critchlow SE, Roux D, Simon MP and Pouysségur J: CD147 subunit of lactate/H+ symporters MCT1 and hypoxia-inducible MCT4 is critical for energetics and growth of glycolytic tumors. Proc Natl Acad Sci USA. 108:16663–16668. 2011. View Article : Google Scholar

29 

Xia X, Lemieux ME, Li W, Carroll JS, Brown M, Liu XS and Kung AL: Integrative analysis of HIF binding and transactivation reveals its role in maintaining histone methylation homeostasis. Proc Natl Acad Sci USA. 106:4260–4265. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu X, Qiao B, Liu Q and Zhang W: Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer. Mol Med Rep 12: 7419-7424, 2015.
APA
Wu, X., Qiao, B., Liu, Q., & Zhang, W. (2015). Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer. Molecular Medicine Reports, 12, 7419-7424. https://doi.org/10.3892/mmr.2015.4410
MLA
Wu, X., Qiao, B., Liu, Q., Zhang, W."Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer". Molecular Medicine Reports 12.5 (2015): 7419-7424.
Chicago
Wu, X., Qiao, B., Liu, Q., Zhang, W."Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer". Molecular Medicine Reports 12, no. 5 (2015): 7419-7424. https://doi.org/10.3892/mmr.2015.4410
Copy and paste a formatted citation
x
Spandidos Publications style
Wu X, Qiao B, Liu Q and Zhang W: Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer. Mol Med Rep 12: 7419-7424, 2015.
APA
Wu, X., Qiao, B., Liu, Q., & Zhang, W. (2015). Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer. Molecular Medicine Reports, 12, 7419-7424. https://doi.org/10.3892/mmr.2015.4410
MLA
Wu, X., Qiao, B., Liu, Q., Zhang, W."Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer". Molecular Medicine Reports 12.5 (2015): 7419-7424.
Chicago
Wu, X., Qiao, B., Liu, Q., Zhang, W."Upregulation of extracellular matrix metalloproteinase inducer promotes hypoxia-induced epithelial-mesenchymal transition in esophageal cancer". Molecular Medicine Reports 12, no. 5 (2015): 7419-7424. https://doi.org/10.3892/mmr.2015.4410
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