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Article Open Access

MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer

  • Authors:
    • Yuefeng Ma
    • Chuanen Bao
    • Ranran Kong
    • Xin Xing
    • Yaya Zhang
    • Shaomin Li
    • Wei Zhang
    • Jiantao Jiang
    • Jin Zhang
    • Zhe Qiao
    • Danjie Zhang
    • Zhenchuan Ma
    • Liangzhang Sun
    • Bin Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Thoracic and Cardiovascular Surgery, The 174th Hospital of The Chinese People's Liberation Army, The Affiliated Chenggong Hospital of Xiamen University, Xiamen, Fujian 710049, P.R. China, Department of Health Care for Cadres, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Oncology, The 174th Hospital of The Chinese People's Liberation Army, The Affiliated Chenggong Hospital of Xiamen University, Xiamen, Fujian 710049, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7367-7373
    |
    Published online on: October 1, 2015
       https://doi.org/10.3892/mmr.2015.4411
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Abstract

The incidence of non‑small cell lung cancer (NSCLC) has significantly increased in China, while the prognosis of affected patients is poor. The pathogenesis of NSCLC is thought to be regulated by microRNAs (miRs). The present study used a miR array in order to determine the expression of miR‑361‑5p, which was significantly lower in NSCLC tissues compared with that in adjacent tissues, indicating a crucial role of miR‑361‑5p during the progression of NSCLC. Furthermore, the effects of transfection-induced upregulation of miR‑361‑5p on the NSCLC cell line H23 were assessed. Overexpression of miR‑361‑5p inhibited the proliferation and colony formation ability of H23 cells. In addition, apoptosis of H23 cells was induced by upregulation of miR‑361‑5p. Furthermore, signal transducer and activator of transcription 6 (STAT6) was confirmed as a direct target of miR‑361‑5p by a dual‑luciferase reporter assay. Moreover, inhibition of STAT6 by small interfering RNA or miR‑361‑5p also decreased the expression of B-cell lymphoma extra large (Bcl-xL). In vivo, miR‑361‑5p significantly reduced tumor growth in a nude mouse xenograft model, and suppressed STAT6 and Bcl-xL expression. In conclusion, the present study indicated that miR‑361‑5p may represent a novel molecular tool for therapeutic and diagnostic strategies in NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Bao C, Kong R, Xing X, Zhang Y, Li S, Zhang W, Jiang J, Zhang J, Qiao Z, Qiao Z, et al: MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer. Mol Med Rep 12: 7367-7373, 2015.
APA
Ma, Y., Bao, C., Kong, R., Xing, X., Zhang, Y., Li, S. ... Zhou, B. (2015). MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer. Molecular Medicine Reports, 12, 7367-7373. https://doi.org/10.3892/mmr.2015.4411
MLA
Ma, Y., Bao, C., Kong, R., Xing, X., Zhang, Y., Li, S., Zhang, W., Jiang, J., Zhang, J., Qiao, Z., Zhang, D., Ma, Z., Sun, L., Zhou, B."MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer". Molecular Medicine Reports 12.5 (2015): 7367-7373.
Chicago
Ma, Y., Bao, C., Kong, R., Xing, X., Zhang, Y., Li, S., Zhang, W., Jiang, J., Zhang, J., Qiao, Z., Zhang, D., Ma, Z., Sun, L., Zhou, B."MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer". Molecular Medicine Reports 12, no. 5 (2015): 7367-7373. https://doi.org/10.3892/mmr.2015.4411
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Bao C, Kong R, Xing X, Zhang Y, Li S, Zhang W, Jiang J, Zhang J, Qiao Z, Qiao Z, et al: MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer. Mol Med Rep 12: 7367-7373, 2015.
APA
Ma, Y., Bao, C., Kong, R., Xing, X., Zhang, Y., Li, S. ... Zhou, B. (2015). MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer. Molecular Medicine Reports, 12, 7367-7373. https://doi.org/10.3892/mmr.2015.4411
MLA
Ma, Y., Bao, C., Kong, R., Xing, X., Zhang, Y., Li, S., Zhang, W., Jiang, J., Zhang, J., Qiao, Z., Zhang, D., Ma, Z., Sun, L., Zhou, B."MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer". Molecular Medicine Reports 12.5 (2015): 7367-7373.
Chicago
Ma, Y., Bao, C., Kong, R., Xing, X., Zhang, Y., Li, S., Zhang, W., Jiang, J., Zhang, J., Qiao, Z., Zhang, D., Ma, Z., Sun, L., Zhou, B."MicroRNA‑361‑5p suppresses cancer progression by targeting signal transducer and activator of transcription 6 in non‑small cell lung cancer". Molecular Medicine Reports 12, no. 5 (2015): 7367-7373. https://doi.org/10.3892/mmr.2015.4411
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