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Article

Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons

  • Authors:
    • Jin Zhu
    • Qingxi Fu
    • Chunfeng Xia
    • Guozhao Ma
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shangdong 250014, P.R. China, Department of Neurology, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China
  • Pages: 8301-8305
    |
    Published online on: October 21, 2015
       https://doi.org/10.3892/mmr.2015.4457
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Abstract

The accumulation of amyloid-β protein (Aβ) is significant in the pathogenesis of Alzheimer's disease. Several previous studies indicate that the NR2B‑containing N‑methyl‑D‑aspartate receptors are critically involved in the Aβ mediated disruption of neuronal function. Diazoxide (DZ), a highly selective drug capable of opening mitochondrial ATP‑sensitive potassium channels, has neuroprotective effects against neuronal cell death. However, the mechanism by which DZ protects cholinergic neurons against Aβ‑induced cytotoxicity remains to be elucidated. The present study was designed to investigate the effects of DZ pretreatment against Aβ1‑42‑induced expression of NR2B in order to gain novel insights into the neuroprotective mechanisms. Following exposure to Aβ1‑42 for 24 h, the expression of the NR2B subunit remained unchanged compared with the control group. However, a significant increase in the expression of the NR2B subunit was observed following treatment with Aβ1‑42 for 72 h (P<0.05); and the upregulation of the expression of the NR2B subunit was reversed by pretreatment with DZ (P<0.05). These results suggested that DZ may counteract Aβ1‑42‑mediated cytotoxicity by alleviating the expression of NR2B.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Fu Q, Xia C and Ma G: Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons. Mol Med Rep 12: 8301-8305, 2015.
APA
Zhu, J., Fu, Q., Xia, C., & Ma, G. (2015). Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons. Molecular Medicine Reports, 12, 8301-8305. https://doi.org/10.3892/mmr.2015.4457
MLA
Zhu, J., Fu, Q., Xia, C., Ma, G."Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons". Molecular Medicine Reports 12.6 (2015): 8301-8305.
Chicago
Zhu, J., Fu, Q., Xia, C., Ma, G."Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons". Molecular Medicine Reports 12, no. 6 (2015): 8301-8305. https://doi.org/10.3892/mmr.2015.4457
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Fu Q, Xia C and Ma G: Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons. Mol Med Rep 12: 8301-8305, 2015.
APA
Zhu, J., Fu, Q., Xia, C., & Ma, G. (2015). Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons. Molecular Medicine Reports, 12, 8301-8305. https://doi.org/10.3892/mmr.2015.4457
MLA
Zhu, J., Fu, Q., Xia, C., Ma, G."Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons". Molecular Medicine Reports 12.6 (2015): 8301-8305.
Chicago
Zhu, J., Fu, Q., Xia, C., Ma, G."Effects of diazoxide on Aβ1-42-induced expression of the NR2B subunit in cultured cholinergic neurons". Molecular Medicine Reports 12, no. 6 (2015): 8301-8305. https://doi.org/10.3892/mmr.2015.4457
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