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Article

Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis

  • Authors:
    • Jiansheng Wang
    • Xiaojun Yang
    • Huan Xiao
    • Jianqiang Kong
    • Miao Bing
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Shanghai Baoshan District Integrated Traditional and Western Medicine Hospital, Shanghai 201900, P.R. China
  • Pages: 8071-8076
    |
    Published online on: October 21, 2015
       https://doi.org/10.3892/mmr.2015.4462
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Abstract

The present study aimed to investigate the gene expression profiles of rats brain tissues treated with halothane compared with untreated controls to improve current understanding of the mechanism of action of the inhaled anesthetic. The GSE357 gene expression profile was dowloaded from the Gene Expression Omnibus database, and included six gene chips of samples repeatedly exposed to halothane and 12 gene chips of untreated controls. The differentially expressed genes (DEGs) between these two groups were identified using the Limma package in R language. Subsequently, the Database for Annotation, Visualization and Integrated Discovery was used to annotate the function of these DEGs. In addition, the most significantly upregulated gene and downregulated gene were annotated, to reveal the functional interactions with other associated genes, in FuncBase database. A total of 44 DEGs were obtained between The control and halothane exposure samples. Following Gene Ontology functional classification, these DEGs were found to be involved predominantly in the circulatory system, regulation of cell proliferation and response to endogenous stimulus and corticosteroid stimulus processes. KRT31 and HMGCS2, which were identified as the most significantly downregulated and upregulated DEGs, respectively, were associated with the lipid metabolic process and T cell activation, respectively. These results provided a basis for the development of improved inhalational anesthetics with minimal side effects and are essential for optimization of inhaled anesthetic techniques for advanced surgical procedures.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Yang X, Xiao H, Kong J and Bing M: Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis. Mol Med Rep 12: 8071-8076, 2015.
APA
Wang, J., Yang, X., Xiao, H., Kong, J., & Bing, M. (2015). Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis. Molecular Medicine Reports, 12, 8071-8076. https://doi.org/10.3892/mmr.2015.4462
MLA
Wang, J., Yang, X., Xiao, H., Kong, J., Bing, M."Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis". Molecular Medicine Reports 12.6 (2015): 8071-8076.
Chicago
Wang, J., Yang, X., Xiao, H., Kong, J., Bing, M."Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis". Molecular Medicine Reports 12, no. 6 (2015): 8071-8076. https://doi.org/10.3892/mmr.2015.4462
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Yang X, Xiao H, Kong J and Bing M: Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis. Mol Med Rep 12: 8071-8076, 2015.
APA
Wang, J., Yang, X., Xiao, H., Kong, J., & Bing, M. (2015). Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis. Molecular Medicine Reports, 12, 8071-8076. https://doi.org/10.3892/mmr.2015.4462
MLA
Wang, J., Yang, X., Xiao, H., Kong, J., Bing, M."Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis". Molecular Medicine Reports 12.6 (2015): 8071-8076.
Chicago
Wang, J., Yang, X., Xiao, H., Kong, J., Bing, M."Determination of the mechanism of action of repetitive halothane exposure on rat brain tissues using a combined method of microarray gene expression profiling and bioinformatics analysis". Molecular Medicine Reports 12, no. 6 (2015): 8071-8076. https://doi.org/10.3892/mmr.2015.4462
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