Effect of Hath1 on the proliferation and apoptosis of cutaneous squamous cell carcinoma in vitro

Ying,Zuolin; Li,Xiaojie; Dang,Hong; Wang,Feng; Xu,Xiaoyan

doi:10.3892/mmr.2015.4463

December-2015 Volume 12 Issue 6

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December-2015 Volume 12 Issue 6

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Article Open Access

Effect of Hath1 on the proliferation and apoptosis of cutaneous squamous cell carcinoma in vitro

Authors:
- Zuolin Ying
- Xiaojie Li
- Hong Dang
- Feng Wang
- Xiaoyan Xu
View Affiliations / Copyright

Affiliations: Department of Dermatology, Shanghai First People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China, Experimental Research Center, Shanghai First People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China

Copyright: © Ying et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 7845-7850
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Published online on: October 21, 2015

https://doi.org/10.3892/mmr.2015.4463
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Abstract

Increasing evidence has demonstrated that the tumor suppressor gene Hath1 is implicated in the development and progression of tumors and is verified to be downregulated in several types of tumor. However, the roles and precise molecular mechanisms of Hath1 in cutaneous squamous cell carcinoma (SCC) remain to be elucidated. In the present study, two approaches were used to investigate the tumor‑suppressing effect of Hath1 in cutaneous SCC. Firstly, the effect of inhibiting Hath1 expression with short hairpin RNA (shRNA) on tumor growth and apoptosis was investigated. KUMA5 cells were stably transfected with a plasmid expressing Hath1 shRNA (pGenesil‑1‑Hath1). Secondly, the anti‑tumor effect of Hath1 was investigated in KUMA5 cells following transfection with pcDNA3.1‑Hath1. The mRNA and protein expression of Hath1 was detected by reverse transcription quantitative polymerase chain reaction and western blot analysis, respectively. Cell proliferation in vitro was assessed using an MTT assay. Flow cytometry was used to detect cell apoptosis. The results demonstrated that compared with the control groups, the expression of Hath1 was significantly reduced in the KUMA5/pGenesil‑1‑Hath1 cells and markedly increased in the KUMA5/pcDNA3.1‑Hath1 cells. Cell proliferation was markedly increased in the KUMA5/pGenesil‑1‑Hath1 cells in a time‑dependent manner; however, it was markedly inhibited in the KUMA5/pcDNA3.1‑Hath1 cells. Flow cytometry revealed that apoptosis decreased in KUMA5/pGenesil‑1‑Hath1 cells and increased in KUMA5/pcDNA3.1‑Hath1 cells. Downregulation of Hath1 expression promoted the proliferation and reduced the apoptosis of KUMA5 cells. By contrast, overexpression of Hath1 inhibited proliferation and induced the apoptosis of KUMA5 cells. These findings provide possible new strategies and therapeutic targets for the treatment and diagnosis of cutaneous SCC.

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