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December-2015 Volume 12 Issue 6

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Article

Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells

  • Authors:
    • Jianyi Niu
    • Gang Ding
    • Li Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Yidu Central Hospital, Weifang Medical University, Qingzhou, Shandong 262500, P.R. China
  • Pages: 8237-8240
    |
    Published online on: October 23, 2015
       https://doi.org/10.3892/mmr.2015.4476
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Abstract

The present study aimed to investigate the effects of simvastatin on the bone differentiation capacity and immunological characteristics of bone marrow mesenchymal stem cells (BMSCs). BMSCs were isolated and cultured in medium containing 1.0 µmol/ml simvastatin. The alkaline phosphatase activity, mRNA expression levels of osteocalcin and bone sialoprotein, and calcium nodule formation were assessed to determine the osteogenic differentiation capability of BMSCs. To investigate alterations in the immunological properties of simvastation‑treated BMSCs, the immunogenicity of these cells and the effect of BMSCs on phytohemagglutinin‑stimulated lymphocyte proliferation were also assessed. Following treatment with simvastatin, the alkaline phosphatase activity, and mRNA expression levels of osteocalcin and bone sialoprotein were increased significantly in the BMSCs. In addition, von Kossa staining revealed a brown calcium‑positive reaction zone in simvastatin‑treated cells. Simvastatin‑induced BMSCs revealed no affect on the proliferation of allogeneic lymphocytes, however, inhibited phytohemagglutinin‑induced lymphocyte proliferation. Collectively, simvastatin promoted the osteogenic differentiation of BMSCs significantly without affecting their immunosuppressive properties.
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1 

Marsh D: Concepts of fracture union, delayed union and non-union. Clin Orthop Relat Res. (Suppl 355): S22–S30. 1998. View Article : Google Scholar

2 

Bishop JA, Palanca AA, Bellino MJ and Lowenberg DW: Assessment of compromised fracture healing. J Am Acad Orthop Surg. 20:273–282. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Obermeyer TS, Yonick D, Lauing K, Stock SR, Nauer R, Strotman P, Shankar R, Gamelli R, Stover M and Callaci JJ: Mesenchymal stem cells facilitate fracture repair in an alcohol-induced impaired healing model. J Orthop Trauma. 26:712–718. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Quarto R, Mastrogiacomo M, Cancedda R, Kutepov SM, Mukhachev V, Lavroukov A, Kon E and Marcacci M: Repair of large bone defects with the use of autologous bone marrow stromal cells. N Engl J Med. 344:385–386. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Dorronsoro A, Fernández-Rueda J, Fechter K, Ferrin I, Salcedo JM, Jakobsson E and Trigueros C: Human mesenchymal stromal cell-mediated immunoregulation: Mechanisms of action and clinical applications. Bone Marrow Res. 2013:2036432013. View Article : Google Scholar : PubMed/NCBI

6 

Krysiak R and Okopien B: Different effects of simvastatin on ex vivo monocyte cytokine release in patients with hypercholesterolemia and impaired glucose tolerance. J Physiol Pharmacol. 61:725–732. 2010.

7 

Mannheim D, Herrmann J, Bonetti PO, Lavi R, Lerman LO and Lerman A: Simvastatin preserves diastolic function in experimental hypercholesterolemia independently of its lipid lowering effect. Atherosclerosis. 216:283–291. 2011. View Article : Google Scholar : PubMed/NCBI

8 

van Nieuw Amerongen GP, Vermeer MA, Nègre-Aminou P, Lankelma J, Emeis JJ and van Hinsbergh VW: Simvastatin improves disturbed endothelial barrier function. Circulation. 102:2803–2809. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Mundy G, Garrett R, Harris S, Chan J, Chen D, Rossini G, Boyce B, Zhao M and Gutierrez G: Stimulation of bone formation in vitro and in rodents by statins. Science. 286:1946–1949. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Fang D, Seo BM, Liu Y, Sonoyama W, Yamaza T, Zhang C, Wang S and Shi S: Transplantation of mesenchymal stem cells is an optimal approach for plastic surgery. Stem Cell. 25:1021–1028. 2007. View Article : Google Scholar

11 

Ding G, Liu Y, An Y, Zhang C, Shi S, Wang W and Wang S: Suppression of T cell proliferation by root apical papilla stem cells in vitro. Cells Tissues Organs. 191:357–364. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Liu G, Shu C, Cui L, Liu W and Cao Y: Tissue-engineered bone formation with cryopreserved human bone marrow mesenchymal stem cells. Cryobiology. 56:209–215. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

14 

Horwitz EM, Gordon PL, Koo WK, Marx JC, Neel MD, McNall RY, Muul L and Hofmann T: Isolated allogeneic bone marrow derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone. Proc Natl Acad Sci USA. 99:8932–8937. 2002. View Article : Google Scholar

15 

Warnke PH, Springer IN, Wiltfang J, Acil Y, Eufinger H, Wehmöller M, Russo PA, Bolte H, Sherry E, Behrens E and Terheyden H: Growth and transplantation of a custom vascularised bone graft in a man. Lancet. 364:766–770. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Hofstetter CP, Schwarz EJ, Hess D, Widenfalk J, El Manira A, Prockop DJ and Olson L: Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery. Proc Natl Acad Sci USA. 99:2199–2204. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Chopp M and Li Y: Treatment of neural injury with marrow stromal cells. Lancet Neurol. 1:92–100. 2002. View Article : Google Scholar

18 

Liu C, Wu Z and Sun HC: The effect of simvastatin on mRNA expression of transforming growth factor-beta1, bone morphogenetic protein-2 and vascular endothelial growth factor in tooth extraction socket. Int J Oral Sci. 1:90–98. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Liu Y, Wang S and Shi S: The role of recipient T cells in mesenchymal stem cell-based tissue regeneration. Int J Biochem Cell Biol. 44:2044–2050. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Le Blanc K, Frassoni F, Ball L, Locatelli F, Roelofs H, Lewis I, Lanino E, Sundberg B, Bernardo ME, Remberger M, et al: Developmental Committee of the European Group for Blood and Marrow Transplantation: Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: A phase II study. Lancet. 371:1579–1586. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Sun L, Akiyama K, Zhang H, Yamaza T, Hou Y, Zhao S, Xu T, Le A and Shi S: Mesenchymal stem cell transplantation reverses multi-organ dysfunction in systemic lupus erythematosus mice and humans. Stem Cells. 27:1421–1432. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Xu J, Wang D, Liu D, Fan Z, Zhang H, Liu O, Ding G, Gao R, Zhang C, Ding Y, et al: Allogeneic mesenchymal stem cell treatment alleviates experimental and clinical Sjögren syndrome. Blood. 120:3142–3151. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Niu J, Ding G and Zhang L: Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells. Mol Med Rep 12: 8237-8240, 2015.
APA
Niu, J., Ding, G., & Zhang, L. (2015). Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells. Molecular Medicine Reports, 12, 8237-8240. https://doi.org/10.3892/mmr.2015.4476
MLA
Niu, J., Ding, G., Zhang, L."Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells". Molecular Medicine Reports 12.6 (2015): 8237-8240.
Chicago
Niu, J., Ding, G., Zhang, L."Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells". Molecular Medicine Reports 12, no. 6 (2015): 8237-8240. https://doi.org/10.3892/mmr.2015.4476
Copy and paste a formatted citation
x
Spandidos Publications style
Niu J, Ding G and Zhang L: Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells. Mol Med Rep 12: 8237-8240, 2015.
APA
Niu, J., Ding, G., & Zhang, L. (2015). Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells. Molecular Medicine Reports, 12, 8237-8240. https://doi.org/10.3892/mmr.2015.4476
MLA
Niu, J., Ding, G., Zhang, L."Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells". Molecular Medicine Reports 12.6 (2015): 8237-8240.
Chicago
Niu, J., Ding, G., Zhang, L."Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells". Molecular Medicine Reports 12, no. 6 (2015): 8237-8240. https://doi.org/10.3892/mmr.2015.4476
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