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Article

MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma

  • Authors:
    • Xian Chen
    • Lianhua Bo
    • Wei Lu
    • Guihua Zhou
    • Qi Chen
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng City No. 1 People's Hospital, Yancheng, Jiangsu 224001, P.R. China
  • Pages: 477-482
    |
    Published online on: November 2, 2015
       https://doi.org/10.3892/mmr.2015.4500
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Abstract

microRNA(miR)-148b has been found to be downregulated in various human malignancies, including hepatocellular carcinoma (HCC) as well as gastric, pancreatic, colon and oral cancer. However, the function of miR‑148b in HCC has remained elusive. The present study examined the effects of miR‑148b on the proliferation, migration and invasion of HCC cells in vitro. After transfection of the HepG2 and SMMC‑7721 HCC cell lines with miR‑148b, an MTT assay, a Transwell migration and invasion assay as well as western blot analysis were performed. miR-148b was shown to inhibit cell proliferation, migration and invasion in the two cell lines. Using a luciferase reporter assay, the present study also provided the first evidence that miR‑148b directly targets Rho‑associated protein kinase 1 in HCC. These results suggested that miR-148 may represent a novel molecular marker and a potential molecular therapeutic for inhibiting metastasis of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Bo L, Lu W, Zhou G and Chen Q: MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma. Mol Med Rep 13: 477-482, 2016.
APA
Chen, X., Bo, L., Lu, W., Zhou, G., & Chen, Q. (2016). MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma. Molecular Medicine Reports, 13, 477-482. https://doi.org/10.3892/mmr.2015.4500
MLA
Chen, X., Bo, L., Lu, W., Zhou, G., Chen, Q."MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma". Molecular Medicine Reports 13.1 (2016): 477-482.
Chicago
Chen, X., Bo, L., Lu, W., Zhou, G., Chen, Q."MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma". Molecular Medicine Reports 13, no. 1 (2016): 477-482. https://doi.org/10.3892/mmr.2015.4500
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Bo L, Lu W, Zhou G and Chen Q: MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma. Mol Med Rep 13: 477-482, 2016.
APA
Chen, X., Bo, L., Lu, W., Zhou, G., & Chen, Q. (2016). MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma. Molecular Medicine Reports, 13, 477-482. https://doi.org/10.3892/mmr.2015.4500
MLA
Chen, X., Bo, L., Lu, W., Zhou, G., Chen, Q."MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma". Molecular Medicine Reports 13.1 (2016): 477-482.
Chicago
Chen, X., Bo, L., Lu, W., Zhou, G., Chen, Q."MicroRNA-148b targets Rho-associated protein kinase 1 to inhibit cell proliferation, migration and invasion in hepatocellular carcinoma". Molecular Medicine Reports 13, no. 1 (2016): 477-482. https://doi.org/10.3892/mmr.2015.4500
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