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Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa

  • Authors:
    • Olivia Herd
    • Flavia Francies
    • Jeffrey Kotzen
    • Trudy Smith
    • Zwide Nxumalo
    • Xanthene Muller
    • Jacobus Slabbert
    • Anne Vral
    • Ans Baeyens
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Biophysics, NRF‑iThemba LABS, Somerset West 7129, South Africa, Department of Radiation Oncology, Charlotte Maxeke Johannesburg Academic Hospital, Johannesburg 2193, South Africa, Department of Obstetrics and Gynaecology, Charlotte Maxeke Johannesburg Academic Hospital, Johannesburg 2193, South Africa, Department of Basic Medical Sciences, Ghent University, Ghent B-9000, Belgium
    Copyright: © Herd et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 130-136
    |
    Published online on: November 5, 2015
       https://doi.org/10.3892/mmr.2015.4504
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Abstract

Cervical cancer is the second most common cancer amongst South African women and is the leading cause of cancer-associated mortality in this region. Several international studies on radiation‑induced DNA damage in lymphocytes of cervical cancer patients have remained inconclusive. Despite the high incidence of cervical cancer in South Africa, and the extensive use of radiotherapy to treat it, the chromosomal radiosensitivity of South African cervical cancer patients has not been studied to date. Since a high number of these patients are human immunodeficiency virus (HIV)‑positive, the effect of HIV infection on chromosomal radiosensitivity was also investigated. Blood samples from 35 cervical cancer patients (20 HIV‑negative and 15 HIV‑positive) and 20 healthy controls were exposed to X‑rays at doses of 6 MV of 2 and 4 Gy in vitro. Chromosomal radiosensitivity was assessed using the micronucleus (MN) assay. MN scores were obtained using the Metafer 4 platform, an automated microscopic system. Three scoring methods of the MNScore module of Metafer were applied and compared. Cervical cancer patients had higher MN values than healthy controls, with HIV‑positive patients having the highest MN values. Differences between groups were significant when using a scoring method that corrects for false positive and false negative MN. The present study suggested increased chromosomal radiosensitivity in HIV-positive South African cervical cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Herd O, Francies F, Kotzen J, Smith T, Nxumalo Z, Muller X, Slabbert J, Vral A and Baeyens A: Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa. Mol Med Rep 13: 130-136, 2016.
APA
Herd, O., Francies, F., Kotzen, J., Smith, T., Nxumalo, Z., Muller, X. ... Baeyens, A. (2016). Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa. Molecular Medicine Reports, 13, 130-136. https://doi.org/10.3892/mmr.2015.4504
MLA
Herd, O., Francies, F., Kotzen, J., Smith, T., Nxumalo, Z., Muller, X., Slabbert, J., Vral, A., Baeyens, A."Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa". Molecular Medicine Reports 13.1 (2016): 130-136.
Chicago
Herd, O., Francies, F., Kotzen, J., Smith, T., Nxumalo, Z., Muller, X., Slabbert, J., Vral, A., Baeyens, A."Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa". Molecular Medicine Reports 13, no. 1 (2016): 130-136. https://doi.org/10.3892/mmr.2015.4504
Copy and paste a formatted citation
x
Spandidos Publications style
Herd O, Francies F, Kotzen J, Smith T, Nxumalo Z, Muller X, Slabbert J, Vral A and Baeyens A: Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa. Mol Med Rep 13: 130-136, 2016.
APA
Herd, O., Francies, F., Kotzen, J., Smith, T., Nxumalo, Z., Muller, X. ... Baeyens, A. (2016). Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa. Molecular Medicine Reports, 13, 130-136. https://doi.org/10.3892/mmr.2015.4504
MLA
Herd, O., Francies, F., Kotzen, J., Smith, T., Nxumalo, Z., Muller, X., Slabbert, J., Vral, A., Baeyens, A."Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa". Molecular Medicine Reports 13.1 (2016): 130-136.
Chicago
Herd, O., Francies, F., Kotzen, J., Smith, T., Nxumalo, Z., Muller, X., Slabbert, J., Vral, A., Baeyens, A."Chromosomal radiosensitivity of human immunodeficiency virus positive/negative cervical cancer patients in South Africa". Molecular Medicine Reports 13, no. 1 (2016): 130-136. https://doi.org/10.3892/mmr.2015.4504
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