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Article

Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells

  • Authors:
    • Jiao Xu
    • Liangbo Lin
    • Min Yong
    • Xiaojing Dong
    • Tinghe Yu
    • Lina Hu
  • View Affiliations / Copyright

    Affiliations: Department of Obsterics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China, Stem Cell Biology and Therapy Laboratory, The Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China, Laboratory of Obsterics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China
  • Pages: 265-272
    |
    Published online on: November 5, 2015
       https://doi.org/10.3892/mmr.2015.4509
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Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) belongs to the adenosine triphosphate‑binding cassette transporter family, members of which are involved in several types of cancer. Previous studies by our group reported that CFTR was highly expressed in serous ovarian cancer (SOC) tissues, and that knockdown of CFTR suppressed the proliferation of ovarian cancer in vitro and in vivo. Thus, the aim of the present study was to construct a recombinant adenoviral vector for the expression of the human CFTR gene in order to study the role of CFTR overexpression in the malignant invasion and migration of SOC cells in vitro. The present study then focused on the mechanisms of the role of CFTR in the migratory and invasive malignant properties of SOC cells. The CFTR gene was inserted into an adenoviral vector by using the AdEasy system in order to obtain the Ad‑CFTR overexpression vector, which was used to transfect the A2780 SOC cell line. Reverse-transcription polymerase chain reaction, western blot analysis and immunofluorescence were performed to detect the expression and localization of CFTR. Cell invasion and motility of the transfected cells compared with those of control cells were observed using Transwell and wound healing assays. A ~4,700 bp fragment of the CFTR gene was confirmed to be correctly cloned in the adenoviral vector and amplification of Ad‑CFTR was observed in HEK293 cells during package. After 48 h of transfection with Ad‑CFTR, ~90% of A2780 cells were red fluorescence protein‑positive. Immunofluorescence showed that following transfection, CFTR expression was increased and CFTR was located in the cell membrane and cytoplasm. CFTR overexpression was shown to enhance the invasion and motility of A2780 cells in vitro. Furthermore, the effects of CFTR overexpression on the activation c‑Src signaling were observed by western blot analysis. CFTR overexpressing cells showed the lowest activity of phospho‑Src (Tyr530), suggesting that CFTR may affect the activation of c‑Src signaling. The results of the present study demonstrated that adenovirus‑mediated CFTR overexpression enhanced cell invasion and motility of SOC cells in vitro. Furthermore, CFTR may be critical for the activation of c‑Src signaling.
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1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Rauh-Hain JA, Krivak TC, Del Carmen MG and Olawaiye AB: Ovarian cancer screening and early detection in the general population. Rev Obstet Gynecol. 4:15–21. 2011.PubMed/NCBI

3 

Lee JM, Trepel JB, Choyke P, et al: CECs and IL-8 have prognostic and predictive utility in patients with recurrent platinum-sensitive ovarian cancer: Biomarker correlates from the randomized phase-2 trial of olaparib and cediranib compared with olaparib in recurrent platinum-sensitive ovarian cancer. Front Oncol. 5:1232015. View Article : Google Scholar

4 

Chambers SK, Clouser MC, Baker AF, et al: Overexpression of tumor vascular endothelial growth factor A may portend an increased likelihood of progression in a phase II trial of bevacizumab and erlotinib in resistant ovarian cancer. Clin Cancer Res. 16:5320–5328. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Rankin EB, Fuh KC, Taylor TE, Krieg AJ, Musser M, Yuan J, et al: AXL is an essential factor and therapeutic target for metastatic ovarian cancer. Cancer Res. 70:7570–7579. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Gottesman MM, Fojo T and Bates SE: Multidrug resistance in cancer: role of ATP-dependent transporters. Nat Rev Cancer. 2:48–58. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Higgins CF: ABC transporters: From microorganisms to man. Annu Rev Cell Biol. 8:67–113. 1992. View Article : Google Scholar : PubMed/NCBI

8 

Bear CE, Li CH, Kartner N, Bridges RJ, Jensen TJ, Ramjeesingh M and Riordan JR: Purification and functional reconstitution of the cystic fibrosis transmembrane conductance regulator (CFTR). Cell. 68:809–818. 1992. View Article : Google Scholar : PubMed/NCBI

9 

Tizzano EF, Silver MM, Chitayat D, Benichou JC and Buchwald M: Differential cellular expression of cystic fibrosis transmembrane regulator in human reproductive tissues. Clues for the infertility in patients with cystic fibrosis. Am J Pathol. 144:906–914. 1994.PubMed/NCBI

10 

Peng X, Wu Z, Yu L, Li J, Xu W, Chan HC, Zhang Y and Hu L: Overexpression of cystic fibrosis transmembrane conductance regulator (CFTR) is associated with human cervical cancer malignancy, progression and prognosis. Gynecol Oncol. 125:470–476. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Xu J, Yong M, Li J, Dong X, Yu T, Fu X and Hu L: High level of CFTR expression is associated with tumor aggression and knockdown of CFTR suppresses proliferation of ovarian cancer in vitro and in vivo. Oncol Rep. 33:2227–2234. 2015.PubMed/NCBI

12 

He TC, Zhou S, da Costa LT, Yu J, Kinzler KW and Vogelstein B: A simplified system for generating recombinant adenoviruses. Proc Natl Acad Sci USA. 95:2509–2514. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

14 

Schwiebert EM, Benos DJ, Egan ME, Stutts MJ and Guggino WB: CFTR is a conductance regulator as well as a chloride channel. Physiol Rev. 79(Suppl): S145–S166. 1999.PubMed/NCBI

15 

Xie C, Jiang XH, Zhang JT, Sun TT, Dong JD, Sanders AJ, Diao RY, Wang Y, Fok KL, Tsang LL, et al: CFTR suppresses tumor progression through miR-193b targeting urokinase plasminogen activator (uPA) in prostate cancer. Oncogene. 32:2282–2291. 2291 e1–7. 2013. View Article : Google Scholar

16 

Zhang JT, Jiang XH, Xie C, Cheng H, Da Dong J, Wang Y, Fok KL, Zhang XH, Sun TT, Tsang LL, et al: Downregulation of CFTR promotes epithelial-to-mesenchymal transition and is associated with poor prognosis of breast cancer. Biochim Biophys Acta. 1833:2961–2969. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Jacquot J, Tabary O, Le Rouzic P and Clement A: Airway epithelial cell inflammatory signalling in cystic fibrosis. Int J Biochem Cell Biol. 40:1703–1715. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Wu Z, Peng X, Li J, Zhang Y and Hu L: Constitutive activation of nuclear factor KB contributes to cystic fibrosis transmembrane conductance regulator expression and promotes human cervical cancer progression and poor prognosis. Int J Gynecol Cancer. 23:906–915. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Verma IM and Weitzman MD: Gene therapy: Twenty-first century medicine. Annu Rev Biochem. 74:711–738. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Björklund A, Kirik D, Rosenblad C, Georgievska B, Lundberg C and Mandel RJ: Towards a neuroprotective gene therapy for Parkinson's disease: use of adenovirus, AAV and lentivirus vectors for gene transfer of GDNF to the nigrostriatal system in the rat Parkinson model. Brain Res. 886:82–98. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Deryugina EI and Quigley JP: Matrix metalloproteinases and tumor metastasis. Cancer Metastasis Rev. 25:9–34. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Brdickova N, Brdicka T, Andera L, Spicka J, Angelisová P, Milgram SL and Horejsí V: Interaction between two adapter proteins, PAG and EBP50: A possible link between membrane rafts and actin cytoskeleton. FEBS Lett. 507:133–136. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Moyer BD, Denton J, Karlson KH, Reynolds D, Wang S, Mickle JE, Milewski M, Cutting GR, Guggino WB, Li M and Stanton BA: A PDZ-interacting domain in CFTR is an apical membrane polarization signal. J Clin Invest. 104:1353–1361. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Martin GS: The hunting of the Src. Nat Rev Mol Cell Biol. 2:467–475. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Summy JM and Gallick GE: Src family kinases in tumor progression and metastasis. Cancer Metastasis Rev. 22:337–358. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Wiener JR, Windham TC, Estrella VC, Parikh NU, Thall PF, Deavers MT, Bast RC, Mills GB and Gallick GE: Activated src protein tyrosine kinase is overexpressed in late-stage human ovarian cancers. Gynecol Oncol. 88:73–79. 2003. View Article : Google Scholar

27 

Pengetnze Y, Steed M, Roby KF, Terranova PF and Taylor CC: Src tyrosine kinase promotes survival and resistance to chemotherapeutics in a mouse ovarian cancer cell line. Biochem Biophys Res Commun. 309:377–383. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Wiener JR, Nakano K, Kruzelock RP, Bucana CD, Bast RC Jr and Gallick GE: Decreased Src tyrosine kinase activity inhibits malignant human ovarian cancer tumor growth in a nude mouse model. Clin Cancer Res. 5:2164–2170. 1999.PubMed/NCBI

29 

Okada M: Regulation of the SRC family kinases by Csk. Int J Biol Sci. 8:1385–1397. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Knighton DR, Xuong NH, Taylor SS and Sowadski JM: Crystallization studies of cAMP-dependent protein kinase. Cocrystals of the catalytic subunit with a 20 amino acid residue peptide inhibitor and MgATP diffract to 30 A resolution. J Mol Biol. 220:217–220. 1991. View Article : Google Scholar : PubMed/NCBI

31 

Cooper JA, Gould KL, Cartwright CA and Hunter T: Tyr527 is phosphorylated in pp60c-src: Implications for regulation. Science. 231:1431–1434. 1986. View Article : Google Scholar : PubMed/NCBI

32 

Takeuchi S, Takayama Y, Ogawa A, Tamura K and Okada M: Transmembrane phosphoprotein Cbp positively regulates the activity of the carboxyl-terminal Src kinase, Csk. J Biol Chem. 275:29183–29186. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu J, Lin L, Yong M, Dong X, Yu T and Hu L: Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells. Mol Med Rep 13: 265-272, 2016.
APA
Xu, J., Lin, L., Yong, M., Dong, X., Yu, T., & Hu, L. (2016). Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells. Molecular Medicine Reports, 13, 265-272. https://doi.org/10.3892/mmr.2015.4509
MLA
Xu, J., Lin, L., Yong, M., Dong, X., Yu, T., Hu, L."Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells". Molecular Medicine Reports 13.1 (2016): 265-272.
Chicago
Xu, J., Lin, L., Yong, M., Dong, X., Yu, T., Hu, L."Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells". Molecular Medicine Reports 13, no. 1 (2016): 265-272. https://doi.org/10.3892/mmr.2015.4509
Copy and paste a formatted citation
x
Spandidos Publications style
Xu J, Lin L, Yong M, Dong X, Yu T and Hu L: Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells. Mol Med Rep 13: 265-272, 2016.
APA
Xu, J., Lin, L., Yong, M., Dong, X., Yu, T., & Hu, L. (2016). Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells. Molecular Medicine Reports, 13, 265-272. https://doi.org/10.3892/mmr.2015.4509
MLA
Xu, J., Lin, L., Yong, M., Dong, X., Yu, T., Hu, L."Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells". Molecular Medicine Reports 13.1 (2016): 265-272.
Chicago
Xu, J., Lin, L., Yong, M., Dong, X., Yu, T., Hu, L."Adenovirus‑mediated overexpression of cystic fibrosis transmembrane conductance regulator enhances invasiveness and motility of serous ovarian cancer cells". Molecular Medicine Reports 13, no. 1 (2016): 265-272. https://doi.org/10.3892/mmr.2015.4509
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