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Article

Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression

  • Authors:
    • Zhichen Pu
    • Min Zhu
    • Fandou Kong
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 555-559
    |
    Published online on: November 6, 2015
       https://doi.org/10.3892/mmr.2015.4512
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Abstract

The mortality rate of ovarian cancer is the highest of all gynecological malignancies. Telmisartan is a commonly used clinical angiotensin receptor blocker, which has antihypertensive, anti‑inflammatory and antithrombotic effects. In the present study, it was investigated whether telmisartan could exert anticancer effects on ovarian cancer cells through upregulating peroxisome proliferator‑activated receptor γ (PPARγ) and downregulating matrix metalloproteinase‑9 (MMP‑9) expression. A 3.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to analyze the proliferation of HEY cells. A Caspase‑3 Activity Assay kit and an Annexin V‑fluorescein isothiocyanate/propidium iodide kit were used to analyze the apoptosis of HEY cells. In addition, a gelatin zymography assay and reverse trancription‑quantitative polymerase chain reaction were included to analyze the expression of PPARγ and MMP‑9 in HEY cells. The data showed that telmisartan could significantly decrease cell viability and induce the apoptosis of HEY cells in a time‑ and dose‑dependent manner. Furthermore, telmisartan could also dose‑dependently increase the expression of PPARγ and decrease the expression of MMP‑9 in HEY cells. In addition, downregulation of the expression of PPARγ by small interfering (si)RNA could reduce the effect of telmisartan on ovarian cancer cells and increase the expression of MMP‑9. In conclusion, the results indicated that telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells by upregulating PPARγ and downregulating MMP‑9 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Pu Z, Zhu M and Kong F: Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression. Mol Med Rep 13: 555-559, 2016.
APA
Pu, Z., Zhu, M., & Kong, F. (2016). Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression. Molecular Medicine Reports, 13, 555-559. https://doi.org/10.3892/mmr.2015.4512
MLA
Pu, Z., Zhu, M., Kong, F."Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression". Molecular Medicine Reports 13.1 (2016): 555-559.
Chicago
Pu, Z., Zhu, M., Kong, F."Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression". Molecular Medicine Reports 13, no. 1 (2016): 555-559. https://doi.org/10.3892/mmr.2015.4512
Copy and paste a formatted citation
x
Spandidos Publications style
Pu Z, Zhu M and Kong F: Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression. Mol Med Rep 13: 555-559, 2016.
APA
Pu, Z., Zhu, M., & Kong, F. (2016). Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression. Molecular Medicine Reports, 13, 555-559. https://doi.org/10.3892/mmr.2015.4512
MLA
Pu, Z., Zhu, M., Kong, F."Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression". Molecular Medicine Reports 13.1 (2016): 555-559.
Chicago
Pu, Z., Zhu, M., Kong, F."Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression". Molecular Medicine Reports 13, no. 1 (2016): 555-559. https://doi.org/10.3892/mmr.2015.4512
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