Endoplasmic reticulum protein 29 is involved in endoplasmic reticulum stress in islet beta cells

Gao,Jialin; Zhang,Yao; Wang,Lizhuo; Xia,Libing; Lu,Meiqing; Zhang,Binghua; Chen,Yueping; He,Lianzhi

doi:10.3892/mmr.2015.4527

January-2016 Volume 13 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

January-2016 Volume 13 Issue 1

Full Size Image

Article

Endoplasmic reticulum protein 29 is involved in endoplasmic reticulum stress in islet beta cells

Authors:
- Jialin Gao
- Yao Zhang
- Lizhuo Wang
- Libing Xia
- Meiqing Lu
- Binghua Zhang
- Yueping Chen
- Lianzhi He
View Affiliations / Copyright

Affiliations: Department of Endocrinology and Genetic Metabolism, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241002, P.R. China, Anhui Province Key Laboratory of Biological Macromolecular Research, Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Gynaecology and Obstetrics, Yijishan Hospital of Wannan Medical Collage, Wuhu, Anhui 241002, P.R. China
Pages: 398-402
|
Published online on: November 6, 2015

https://doi.org/10.3892/mmr.2015.4527
Expand metrics +

Abstract

Endoplasmic reticulum stress (ERS) is correlated with insulin resistance and islet‑cell function. In the present study, the sub‑cellular localization and role of ER protein 29 (Erp29) were investigated in an in vitro ERS model of islet beta cells. The INS‑1 islet cell line was treated with various concentrations of tunicamycin to establish the ERS model. Immunofluorescence microscopy demonstrated that Erp29 and anti‑ER marker protein calnexin were co‑localized in NIH3T3 cells, suggesting that Erp29 is localized to the ER. The ERS model induced by tunicamycin showed significantly increased expression of binding immunoglobulin protein (BIP)/glucose‑regulated protein 78 (Grp78), which is a marker for ERS, and the expression of Erp29 was also increased at the mRNA and protein levels. Of note, ERS was blocked following small interfering RNA‑mediated silencing of Erp29 expression, as indicated by reduced BIP/Grp78 expression. As an ER protein, Erp29 may have an important role in ERS in islet beta cells.

View Figures

View References

1	Ozcan U, Cao Q, Yilmaz E, Lee AH, Iwakoshi NN, Ozdelen E, Tuncman G, Görgün C, Glimcher LH and Hotamisligil GS: Endoplasmic reticulum stress links obesity, insulin action, and type 2 diabetes. Science. 306:457–461. 2004. View Article : Google Scholar : PubMed/NCBI
2	Zhao L, Guo H, Chen H, Petersen RB, Zheng L, Peng A and Huang K: Effect of liraglutide on endoplasmic reticulum stress in diabetes. Biochem Biophys Res Commun. 441:133–138. 2013. View Article : Google Scholar : PubMed/NCBI
3	Ou HY, Wu HT, Hung HC, Yang YC, Wu JS and Chang CJ: Endoplasmic reticulum stress induces the expression of fetuin-A to develop insulin resistance. Endocrinology. 153:2974–2984. 2012. View Article : Google Scholar : PubMed/NCBI
4	Sargsyan E, Baryshev M, Backlund M, Sharipo A and Mkrtchian S: Genomic organization and promoter characterization of the gene encoding a putative endoplasmic reticulum chaperone, ERp29. Gene. 285:127–139. 2002. View Article : Google Scholar : PubMed/NCBI
5	Barnes JA and Smoak IW: Glucose-regulated protein 78 (GRP78) is elevated in embryonic mouse heart and induced following hypoglycemic stress. Anat Embryol (Berl). 202:67–74. 2000. View Article : Google Scholar
6	Qian Y, Falahatpisheh MH, Zheng Y, Ramos KS and Tiffany-Castiglioni E: Induction of 78 kD glucose-regulated protein (GRP78) expression and redox-regulated transcription factor activity by lead and mercury in C6 rat glioma cells. Neurotox Res. 3:581–589. 2001. View Article : Google Scholar
7	Ma KX, Chen GW, Shi CY, Cheng FF, Dou H, Feng CC and Liu DZ: Molecular characterization of the glucose-regulated protein 78 (GRP78) gene in planarian Dugesia japonica. Comp Biochem Physiol B Biochem Mol Biol. 171:12–17. 2014. View Article : Google Scholar : PubMed/NCBI
8	Gao J, Xia L, Lu M, Zhang B, Chen Y, Xu R and Wang L: TM7SF1 (GPR137B): A novel lysosome integral membrane protein. Mol Biol Rep. 39:8883–8889. 2012. View Article : Google Scholar : PubMed/NCBI
9	Sargsyan E, Baryshev M, Szekely L, Sharipo A and Mkrtchian S: Identification of ERp29, an endoplasmic reticulum lumenal protein, as a new member of the thyroglobulin folding complex. J Biol Chem. 277:17009–17015. 2002. View Article : Google Scholar : PubMed/NCBI
10	Marchetti P, Bugliani M, Lupi R, Marselli L, Masini M, Boggi U, Filipponi F, Weir GC, Eizirik DL and Cnop M: The endoplasmic reticulum in pancreatic beta cells of type 2 diabetes patients. Diabetologia. 50:2486–2494. 2007. View Article : Google Scholar : PubMed/NCBI
11	Zhu M, Guo M, Fei L, Pan XQ and Liu QQ: 4-phenylbutyric acid attenuates endoplasmic reticulum stress-mediated pancreatic beta-cell apoptosis in rats with streptozotocin-induced diabetes. Endocrine. 47:129–137. 2014. View Article : Google Scholar
12	Zhang B, Wang M, Yang Y, Wang Y, Pang X, Su Y, Wang J, Ai G and Zou Z: ERp29 is a radiation-responsive gene in IEC-6 cell. J Radiat Res. 49:587–596. 2008. View Article : Google Scholar : PubMed/NCBI