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Article

Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation

  • Authors:
    • Xiaoxu Wang
    • Shiyu Li
    • Xiaoran Lu
    • Pan Hu
    • Haiyan Chen
    • Zheng Li
    • Zhaoyang Bu
    • Xulong Lang
    • Xinglong Wang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, P.R. China, Changchun University of Science and Technology, Changchun, Jilin 130022, P.R. China
  • Pages: 257-264
    |
    Published online on: November 9, 2015
       https://doi.org/10.3892/mmr.2015.4532
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Abstract

Rapid and efficient inactivation of a target gene in Escherichia coli chromosomes is required to investigate metabolic engineering. In the present study, a multiple gene inactivation approach was demonstrated in four strains of enterotoxigenic E. coli (ETEC), which are the predominant pathogenic bacteria causing piglet diarrhea, mediated by λ Red and Xer recombination. The chromosomal genes, luxS and pfs were inactivated using the multiple gene inactivation approach in the wild‑type strains of E. coli, K88, K99, 987P and F41. This indicated that dif sites may be reused to inactivate multiple chromosomal genes when no antibiotic‑resistant selectable markers remain. Following inactivation of luxS and pfs, the ability of ETEC to produce the quorum sensing signal, and induce auto‑inducer 2 activity and biofilm formation were significantly reduced. Furthermore, the multiple gene inactivation approach also exhibits a high recombination efficiency and follows a simple process.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Li S, Lu X, Hu P, Chen H, Li Z, Bu Z, Lang X and Wang X: Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation. Mol Med Rep 13: 257-264, 2016.
APA
Wang, X., Li, S., Lu, X., Hu, P., Chen, H., Li, Z. ... Wang, X. (2016). Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation. Molecular Medicine Reports, 13, 257-264. https://doi.org/10.3892/mmr.2015.4532
MLA
Wang, X., Li, S., Lu, X., Hu, P., Chen, H., Li, Z., Bu, Z., Lang, X., Wang, X."Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation". Molecular Medicine Reports 13.1 (2016): 257-264.
Chicago
Wang, X., Li, S., Lu, X., Hu, P., Chen, H., Li, Z., Bu, Z., Lang, X., Wang, X."Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation". Molecular Medicine Reports 13, no. 1 (2016): 257-264. https://doi.org/10.3892/mmr.2015.4532
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Li S, Lu X, Hu P, Chen H, Li Z, Bu Z, Lang X and Wang X: Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation. Mol Med Rep 13: 257-264, 2016.
APA
Wang, X., Li, S., Lu, X., Hu, P., Chen, H., Li, Z. ... Wang, X. (2016). Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation. Molecular Medicine Reports, 13, 257-264. https://doi.org/10.3892/mmr.2015.4532
MLA
Wang, X., Li, S., Lu, X., Hu, P., Chen, H., Li, Z., Bu, Z., Lang, X., Wang, X."Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation". Molecular Medicine Reports 13.1 (2016): 257-264.
Chicago
Wang, X., Li, S., Lu, X., Hu, P., Chen, H., Li, Z., Bu, Z., Lang, X., Wang, X."Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation". Molecular Medicine Reports 13, no. 1 (2016): 257-264. https://doi.org/10.3892/mmr.2015.4532
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