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KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54

  • Authors:
    • Chun‑Hui Wang
    • Chong Qiao
    • Ruo‑Chen Wang
    • Wen‑Ping Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, P.R. China, Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China, Liaoning Province Shiyan High School, Shenyang, Liaoning 110841, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 123-129
    |
    Published online on: November 9, 2015
       https://doi.org/10.3892/mmr.2015.4535
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Abstract

The onset of local invasion and lymphatic metastasis in pancreatic cancer limits survival following surgical intervention and additional therapies. Reduced expression of KiSS‑1 in pancreatic cancer is associated with cancer metastasis. Previous studies have indicated that kisspeptin, the KiSS‑1 peptide, is able to bind to its receptor‑GPR54 (hOT7T175) and suppress the migration of PANC‑1 pancreatic cancer cells. Whether the metastatic suppression of KiSS‑1 is dependent on the levels of GPR54 in pancreatic cancer cell lines remains unclear. Human BxPC‑3 pancreatic carcinoma cells are highly differentiated without exhibiting metastasis, however PANC‑1 pancreatic carcinoma cells are poorly differentiated and exhibit local and lymph node metastasis. Compared with primary cultured trophoblasts, BxPc‑3 and PANC‑1 cells were observed to express low levels of KiSS‑1 mRNA and protein, measured using reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. However, greater mRNA and protein expression levels of GPR54 were observed in PANC‑1 cells compared with BxPc‑3 cells. An MTT assay was used to investigate the effect of KiSS‑1 on BxPc‑3 and PANC‑1 cell proliferation. There were no significant differences in proliferation following transfection with KiSS‑1 in BxPc‑3 and PANC‑1 cells compared with the controls (P>0.05). A Transwell assay with chambers coated with Matrigel was used to evaluate the in vitro invasive ability of BxPc‑3 and PANC‑1 cells, with the invasion index of BxPc‑3 and PANC‑1 cells significantly reduced following 48 h of KiSS‑1 overexpression (P<0.05). The mRNA and protein expression levels of KiSS‑1 were significantly increased in BxPc‑3 and PANC‑1 cells 48 h subsequent to transfection with KiSS‑1 (P<0.05), while GPR54 expression was not altered (P>0.05). KiSS‑1 is a metastasis suppressor gene of pancreatic cancer, and this suppression is not dependent on the expression levels of GPR54. Therefore, KiSS‑1 is potentially a novel target for gene therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Wang CH, Qiao C, Wang RC and Zhou WP: KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54. Mol Med Rep 13: 123-129, 2016.
APA
Wang, C., Qiao, C., Wang, R., & Zhou, W. (2016). KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54. Molecular Medicine Reports, 13, 123-129. https://doi.org/10.3892/mmr.2015.4535
MLA
Wang, C., Qiao, C., Wang, R., Zhou, W."KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54". Molecular Medicine Reports 13.1 (2016): 123-129.
Chicago
Wang, C., Qiao, C., Wang, R., Zhou, W."KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54". Molecular Medicine Reports 13, no. 1 (2016): 123-129. https://doi.org/10.3892/mmr.2015.4535
Copy and paste a formatted citation
x
Spandidos Publications style
Wang CH, Qiao C, Wang RC and Zhou WP: KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54. Mol Med Rep 13: 123-129, 2016.
APA
Wang, C., Qiao, C., Wang, R., & Zhou, W. (2016). KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54. Molecular Medicine Reports, 13, 123-129. https://doi.org/10.3892/mmr.2015.4535
MLA
Wang, C., Qiao, C., Wang, R., Zhou, W."KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54". Molecular Medicine Reports 13.1 (2016): 123-129.
Chicago
Wang, C., Qiao, C., Wang, R., Zhou, W."KiSS‑1‑mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS‑1 receptor GPR54". Molecular Medicine Reports 13, no. 1 (2016): 123-129. https://doi.org/10.3892/mmr.2015.4535
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