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Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells

  • Authors:
    • Lili Qiao
    • Ning Liang
    • Jian Xie
    • Hui Luo
    • Jingxin Zhang
    • Guodong Deng
    • Yupeng Li
    • Jiandong Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Graduate School, Weifang Medical College, Weifang, Shandong 261053, P.R. China, Graduate School, Taishan Medical College, Taian, Shandong 271021, P.R. China
    Copyright: © Qiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 160-166
    |
    Published online on: November 10, 2015
       https://doi.org/10.3892/mmr.2015.4543
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Abstract

Galectin-3 is a multifunctional β-galactoside‑binding lectin that is involved in multiple biological functions which are upregulated in malignancies, including cell growth, adhesion, proliferation, progression and metastasis, as well as apoptosis. A previous study has confirmed the roles of galecin-3 overexpression in the biological behavior of Eca109 human esophageal cancer (EC) cells. In the present study, small interfering (si)RNA-mediated galectin-3 silencing was performed to analyze the effects of decreased galectin-3 expression on the biological behavior of EC cells. Western blot and quantitative polymerase chain reaction analyses were utilized to confirm galectin-3 knockdown at the protein and mRNA level (P<0.05 vs. siRNA-control and untransfected groups). Cell proliferation was assessed using the Cell Counting Kit-8 assay. At 72 and 96 h after transfection, the proliferation of Eca109 cells in the siRNA-Gal-3 group was decreased compared with that in the siRNA-Control and untransfected groups (P<0.001 and P=0.004, respectively). Furthermore, Transwell assays demonstrated that inhibition of galecin-3 significantly reduced the migration and invasion of Eca109 cells compared with that in the other groups (P<0.05). Finally, apoptosis of Eca109 cells was detected using Annexin V/7-amino‑actinomycin double-staining and flow cytometric analysis. Galectin-3 knockdown significantly enhanced the apoptotic rate of Eca109 cells compared with that in the siRNA-control and untreated groups (P=0.031 and P=0.047, respectively). In conclusion, following successful knockdown of galecin-3 expression in Eca109 cells, the cell proliferation, migration and invasion were reduced, while the apoptosis was enhanced, which indicates that galectin silencing may represent a therapeutic strategy for EC.
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Copy and paste a formatted citation
Spandidos Publications style
Qiao L, Liang N, Xie J, Luo H, Zhang J, Deng G, Li Y and Zhang J: Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells. Mol Med Rep 13: 160-166, 2016.
APA
Qiao, L., Liang, N., Xie, J., Luo, H., Zhang, J., Deng, G. ... Zhang, J. (2016). Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells. Molecular Medicine Reports, 13, 160-166. https://doi.org/10.3892/mmr.2015.4543
MLA
Qiao, L., Liang, N., Xie, J., Luo, H., Zhang, J., Deng, G., Li, Y., Zhang, J."Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells". Molecular Medicine Reports 13.1 (2016): 160-166.
Chicago
Qiao, L., Liang, N., Xie, J., Luo, H., Zhang, J., Deng, G., Li, Y., Zhang, J."Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells". Molecular Medicine Reports 13, no. 1 (2016): 160-166. https://doi.org/10.3892/mmr.2015.4543
Copy and paste a formatted citation
x
Spandidos Publications style
Qiao L, Liang N, Xie J, Luo H, Zhang J, Deng G, Li Y and Zhang J: Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells. Mol Med Rep 13: 160-166, 2016.
APA
Qiao, L., Liang, N., Xie, J., Luo, H., Zhang, J., Deng, G. ... Zhang, J. (2016). Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells. Molecular Medicine Reports, 13, 160-166. https://doi.org/10.3892/mmr.2015.4543
MLA
Qiao, L., Liang, N., Xie, J., Luo, H., Zhang, J., Deng, G., Li, Y., Zhang, J."Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells". Molecular Medicine Reports 13.1 (2016): 160-166.
Chicago
Qiao, L., Liang, N., Xie, J., Luo, H., Zhang, J., Deng, G., Li, Y., Zhang, J."Gene silencing of galectin-3 changes the biological behavior of Eca109 human esophageal cancer cells". Molecular Medicine Reports 13, no. 1 (2016): 160-166. https://doi.org/10.3892/mmr.2015.4543
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