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Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis

  • Authors:
    • Siyuan Kong
    • Jinxue Ruan
    • Leilei Xin
    • Junhua Fan
    • Jihan Xia
    • Zhiguo Liu
    • Yulian Mu
    • Shulin Yang
    • Kui Li
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China
    Copyright: © Kong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 669-680
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    Published online on: November 19, 2015
       https://doi.org/10.3892/mmr.2015.4582
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Abstract

There are currently no multi‑transgenic minipig models of diabetes for the regulation of multiple genes involved in its pathogenesis. The foot and mouth disease virus 2A (F2A)‑mediated polycistronic system possesses several advantages, and the present study developed a novel multi‑transgenic minipig model associated with diabetes using this system. The tissue‑specific polycistronic system used in the present study consisted of two expression cassettes, separated by an insulator: (i) 11‑β‑hydroxysteroid dehydrogenase 1 (11β‑HSD1), driven by the porcine liver‑specific apolipoprotein E promoter; (ii) human islet amyloid polypeptide (hIAPP) and C/EBP homologous protein (CHOP), linked to the furin digested site and F‑2A, driven by the porcine pancreas‑specific insulin promoter. In the present study, porcine fetal fibroblasts were transfected with this vector. Following somatic cell nuclear transfer using 10 cell clones and the transplantation of 1,459 embryos in total, three Landrace x Yorkshire surrogates became pregnant and delivered three Wuzhishan piglets. Genomic polymerase chain reaction (PCR) demonstrated that the piglets were multi‑transgenic. Reverse transcription‑quantitative PCR confirmed that 11β‑HSD1 transcription was upregulated in the targeted liver. Similarly, hIAPP and CHOP were expressed at high levels, compared with the control (P<0.05 and P<0.01) in the pancreas, consistent with the western blotting and immunohistochemistry results. The primary results also showed that overexpression of 11β‑HSD1 in the liver increased the liver fat lipid parameters; and the levels of hIAPP and CHOP in the pancreatic islet cells, leading to delayed β‑cell development and apoptosis. This novel tissue‑specific polycistronic system offers a promising starting point for efficiently mimicking multigenic metabolic disease.
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1 

American Diabetes Association: Diagnosis and classification of diabetes mellitus. Diabetes care. 36(Suppl 1): S67–S74. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Masiello P: Animal models of type 2 diabetes with reduced pancreatic beta-cell mass. Int J Biochem Cell Biol. 38:873–893. 2006. View Article : Google Scholar

3 

Wolf E, Braun-Reichhart C, Streckel E and Renner S: Genetically engineered pig models for diabetes research. Transgenic Res. 23:27–38. 2014. View Article : Google Scholar

4 

Rüster C and Wolf G: Models of diabetic nephropathy. Drug discovery today: Disease models. 7:35–41. 2010.

5 

Verma N, Rettenmeier AW and Schmitz-Spanke S: Recent advances in the use of Sus scrofa (pig) as a model system for proteomic studies. Proteomics. 11:776–793. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Liu H, Li Y, Wei Q, Liu C, Bolund L, Vajta G, Dou H, Yang W, Xu Y, Luan J, et al: Development of transgenic minipigs with expression of antimorphic human cryptochrome 1. PLoS One. 8:e760982013. View Article : Google Scholar : PubMed/NCBI

7 

Xia JH, Yuan J, Xin LL, Zhang Y, Kong S, Chen Y, Yang S and Li K: Transcriptome analysis on the inflammatory cell infiltration of nonalcoholic steatohepatitis in Bama minipigs induced by a long-term high-fat, high-sucrose diet. Plos One. 9:e1137242014. View Article : Google Scholar : PubMed/NCBI

8 

Xia JH, Zhang YY, Xin LL, Kong S, Chen Y, Yang S and Li K: Global Transcriptomic Profiling of Cardiac Hypertrophy and Fatty Heart Induced by Long-Term High-Energy Diet in Bama Miniature Pigs. Plos One. 10:e01324202015. View Article : Google Scholar : PubMed/NCBI

9 

Yang SL, Xia JH, Zhang YY, Fan JG, Wang H, Yuan J, Zhao ZZ, Pan Q, Mu YL, Xin LL, Chen YX and Li K: Hyperinsulinemia shifted energy supply from glucose to ketone bodies in early nonalcoholic steatohepatitis from high-fat high-sucrose diet induced Bama minipigs. Sci Rep. 5:139802015. View Article : Google Scholar : PubMed/NCBI

10 

Winzell MS and Ahrén B: The high-fat diet-fed mouse - A model for studying mechanisms and treatment of impaired glucose tolerance and type 2 diabetes. Diabetes. 53:S215–S219. 2004. View Article : Google Scholar

11 

Danda RS, Habiba NM, Rincon-Choles H, Bhandari BK, Barnes JL, Abboud HE and Pergola PE: Kidney involvement in a nongenetic rat model of type 2 diabetes. Kidney Int. 68:2562–2571. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Deng W, Yang D, Zhao B, Ouyang Z, Song J, Fan N, Liu Z, Zhao Y, Wu Q, Nashun B, et al: Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer. PLoS One. 6:e199862011. View Article : Google Scholar : PubMed/NCBI

13 

Dieckhoff B, Kessler B, Jobst D, Kues W, Petersen B, Pfeifer A, Kurth R, Niemann H, Wolf E and Denner J: Distribution and expression of porcine endogenous retroviruses in multi-transgenic pigs generated for xenotransplantation. Xenotransplantation. 16:64–73. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Webster NL, Forni M, Bacci ML, Giovannoni R, Razzini R, Fantinati P, Zannoni A, Fusetti L, Dalprà L, Bianco MR, et al: Multi-transgenic pigs expressing three fluorescent proteins produced with high efficiency by sperm mediated gene transfer. Mol Reprod Dev. 72:68–76. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Jeong YH, Park CH, Jang GH, Jeong YI, Hwang IS, Jeong YW, Kim YK, Shin T, Kim NH, Hyun SH, et al: Production of multiple transgenic Yucatan miniature pigs expressing human complement regulatory factors, human CD55, CD59 and H-transferase genes. PLoS One. 8:e632412013. View Article : Google Scholar

16 

Park SJ, Cho B, Koo OJ, Kim H, Kang JT, Hurh S, Kim SJ, Yeom HJ, Moon J, Lee EM, et al: Production and characterization of soluble human TNFRI-Fc and human HO-1 (HMOX1) transgenic pigs by using the F2A peptide. Transgenic Res. 23:407–419. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Renner S, Braun-Reichhart C, Blutke A, Herbach N, Emrich D, Streckel E, Wünsch A, Kessler B, Kurome M, Bähr A, et al: Permanent neonatal diabetes in INS (C94Y) transgenic pigs. Diabetes. 62:1505–1511. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Umeyama K, Watanabe M, Saito H, Kurome M, Tohi S, Matsunari H, Miki K and Nagashima H: Dominant-negative mutant hepatocyte nuclear factor 1alpha induces diabetes in transgenic-cloned pigs. Transgenic Res. 18:697–706. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Renner S, Fehlings C, Herbach N, Hofmann A, von Waldthausen DC, Kessler B, Ulrichs K, Chodnevskaja I, Moskalenko V, Amselgruber W, et al: Glucose intolerance and reduced proliferation of pancreatic beta-cells in transgenic pigs with impaired glucose-dependent insulinotropic polypeptide function. Diabetes. 59:1228–1238. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Masuzaki H, Paterson J, Shinyama H, Morton NM, Mullins JJ, Seckl JR and Flier JS: A transgenic model of visceral obesity and the metabolic syndrome. Science. 294:2166–2170. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Mathis D, Vence L and Benoist C: beta-cell death during progression to diabetes. Nature. 414:792–798. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Maris M, Overbergh L, Gysemans C, Waget A, Cardozo AK, Verdrengh E, Cunha JP, Gotoh T, Cnop M, et al: Deletion of C/EBP homologous protein (Chop) in C57Bl/6 mice dissociates obesity from insulin resistance. Diabetologia. 55:1167–1178. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Hoppener JWM, Jacobs HM, Wierup N, Sotthewes G, Sprong M, de Vos P, Berger R, Sundler F and Ahrén B: Human islet amyloid polypeptide transgenic mice: In vivo and ex vivo models for the role of hIAPP in type 2 diabetes mellitus. Exp Diabetes Res. 2008:6970352008. View Article : Google Scholar : PubMed/NCBI

24 

Paterson JM, Morton NM, Fievet C, Kenyon CJ, Holmes MC, Staels B, Seckl JR and Mullins JJ: Metabolic syndrome without obesity: Hepatic overexpression of 11beta-hydroxysteroid dehydrogenase type 1 in transgenic mice. Proc Natl Acad Sci USA. 101:7088–7093. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Hull RL, Shen ZP, Watts MR, Kodama K, Carr DB, Utzschneider KM, Zraika S, Wang F and Kahn SE: Long-term treatment with rosiglitazone and metformin reduces the extent of, but does not prevent, islet antyloid deposition in mice expressing the gene for human islet antyloid polypeptide. Diabetes. 54:2235–2244. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Masuzaki H and Flier JS: Tissue-specific glucocorticoid reactivating enzyme, 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1) - a promising drug target for the treatment of metabolic syndrome. Curr Drug Targets Immune Endocr Metabol Disord. 3:255–262. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Chen ZY, Liu SN, Li CN, et al: Atorvastatin helps preserve pancreatic beta cell function in obese C57BL/6 J mice and the effect is related to increased pancreas proliferation and amelioration of endoplasmic-reticulum stress. Lipids Health Dis. 13:2014. View Article : Google Scholar

28 

West AG, Gaszner M and Felsenfeld G: Insulators: Many functions, many mechanisms. Genes Dev. 16:271–288. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Xia J, Hu B, Mu Y, Xin L, Yang S and Li K: Molecular cloning and characterization of the promoter region of the porcine apolipoprotein E gene. Mol Biol Rep. 41:3211–3217. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Ruan JX, Li HG, Xu K, Wu T, Wei J, Zhou R, Liu Z, Mu Y, Yang S, Ouyang H, et al: Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs. Sci Rep. 5:142532015. View Article : Google Scholar : PubMed/NCBI

31 

Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, et al: Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science. 295:1089–1092. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods. 25:402–408. 2001. View Article : Google Scholar

33 

Bubner B and Baldwin IT: Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Rep. 23:263–271. 2004. View Article : Google Scholar : PubMed/NCBI

34 

Luo W, Li Z, Huang Y, Han Y, Yao C, Duan X, Ouyang H and Li L: Generation of AQP2-Cre transgenic mini-pigs specifically expressing Cre recombinase in kidney collecting duct cells. Transgenic Res. 23:365–375. 2014. View Article : Google Scholar

35 

Li L, Li Q, Bao Y, Li J, Chen Z, Yu X, Zhao Y, Tian K and Li N: RNAi-based inhibition of porcine reproductive and respiratory syndrome virus replication in transgenic pigs. J Biotechnol. 171:17–24. 2014. View Article : Google Scholar

36 

Zhang Y, Wong CH, Birnbaum RY, Li G, Favaro R, Ngan CY, Lim J, Tai E, Poh HM, Wong E, et al: Chromatin connectivity maps reveal dynamic promoter-enhancer long-range associations. Nature. 504:306–310. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Sadeghnia HR, Ghorbani Hesari T, Mortazavian SM, Mousavi SH, Tayarani-Najaran Z and Ghorbani A: Viola tricolor induces apoptosis in cancer cells and exhibits antiangiogenic activity on chicken chorioallantoic membrane. Biomed Res Int. 2014:6257922014. View Article : Google Scholar : PubMed/NCBI

38 

Rabhi N, Salas E, Froguel P and Annicotte JS: Role of the unfolded protein response in β cell compensation and failure during diabetes. J Diabetes Res. 2014:7951712014. View Article : Google Scholar

39 

Morton NM, Paterson JM, Masuzaki H, Holmes MC, Staels B, Fievet C, Walker BR, Flier JS, Mullins JJ and Seckl JR: Novel adipose tissue-mediated resistance to diet-induced visceral obesity in 11 beta-hydroxysteroid dehydrogenase type 1-deficient mice. Diabetes. 53:931–938. 2004. View Article : Google Scholar : PubMed/NCBI

40 

Paterson JM, Holmes MC, Kenyon CJ, Carter R, Mullins JJ and Seckl JR: Liver-selective transgene rescue of hypothalamic-pituitary-adrenal axis dysfunction in 11 beta-hydroxysteroid dehydrogenase type 1-deficient mice. Endocrinology. 148:961–966. 2007. View Article : Google Scholar

41 

Lee AW and Cox RD: Use of mouse models in studying type 2 diabetes mellitus. Expert Rev Mol Med. 13:e12011. View Article : Google Scholar : PubMed/NCBI

42 

Jung EM, An BS, Kim YK, Jeong YH, Hwang WS and Jeung EB: Generation of porcine fibroblasts overexpressing 11β-HSD1 with adipose tissue-specific aP2 promoter as a porcine model of metabolic syndrome. Mol Med Rep. 8:751–756. 2013.PubMed/NCBI

43 

Jeon Y, Kim Y, Yoon J, Cai L, Hwang SU, Kim E, Lee S, Jeung EB and Hyun SH: Production of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) over-expressed pigs for the study of metabolic syndrome disease. Reprod Fertil Dev. 26:116. 2014. View Article : Google Scholar

44 

Bram Y, Frydman-Marom A, Yanai I, Gilead S, Shaltiel-Karyo R, Amdursky N and Gazit E: Apoptosis induced by islet amyloid polypeptide soluble oligomers is neutralized by diabetes-associated specific antibodies. Sci Rep. 4:42672014. View Article : Google Scholar : PubMed/NCBI

45 

Hull RL, Andrikopoulos S, Verchere CB, Vidal J, Wang F, Cnop M, Prigeon RL and Kahn SE: Increased dietary fat promotes islet amyloid formation and beta-cell secretory dysfunction in a transgenic mouse model of islet amyloid. Diabetes. 52:372–379. 2003. View Article : Google Scholar : PubMed/NCBI

46 

Oyadomari S and Mori M: Roles of CHOP/GADD153 in endoplasmic reticulum stress. Cell Death Differ. 11:381–389. 2004. View Article : Google Scholar

47 

Marchetti P, Bugliani M, Lupi R, Marselli L, Masini M, Boggi U, Filipponi F, Weir GC, Eizirik DL and Cnop M: The endoplasmic reticulum in pancreatic beta cells of type 2 diabetes patients. Diabetologia. 50:2486–2494. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kong S, Ruan J, Xin L, Fan J, Xia J, Liu Z, Mu Y, Yang S and Li K: Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis. Mol Med Rep 13: 669-680, 2016.
APA
Kong, S., Ruan, J., Xin, L., Fan, J., Xia, J., Liu, Z. ... Li, K. (2016). Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis. Molecular Medicine Reports, 13, 669-680. https://doi.org/10.3892/mmr.2015.4582
MLA
Kong, S., Ruan, J., Xin, L., Fan, J., Xia, J., Liu, Z., Mu, Y., Yang, S., Li, K."Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis". Molecular Medicine Reports 13.1 (2016): 669-680.
Chicago
Kong, S., Ruan, J., Xin, L., Fan, J., Xia, J., Liu, Z., Mu, Y., Yang, S., Li, K."Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis". Molecular Medicine Reports 13, no. 1 (2016): 669-680. https://doi.org/10.3892/mmr.2015.4582
Copy and paste a formatted citation
x
Spandidos Publications style
Kong S, Ruan J, Xin L, Fan J, Xia J, Liu Z, Mu Y, Yang S and Li K: Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis. Mol Med Rep 13: 669-680, 2016.
APA
Kong, S., Ruan, J., Xin, L., Fan, J., Xia, J., Liu, Z. ... Li, K. (2016). Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis. Molecular Medicine Reports, 13, 669-680. https://doi.org/10.3892/mmr.2015.4582
MLA
Kong, S., Ruan, J., Xin, L., Fan, J., Xia, J., Liu, Z., Mu, Y., Yang, S., Li, K."Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis". Molecular Medicine Reports 13.1 (2016): 669-680.
Chicago
Kong, S., Ruan, J., Xin, L., Fan, J., Xia, J., Liu, Z., Mu, Y., Yang, S., Li, K."Multi‑transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis". Molecular Medicine Reports 13, no. 1 (2016): 669-680. https://doi.org/10.3892/mmr.2015.4582
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