Inhibitory effect of withaferin A on Helicobacter pylori‑induced IL‑8 production and NF‑κB activation in gastric epithelial cells

  • Authors:
    • Green Kim
    • Tae‑Hyoun Kim
    • Min‑Jung Kang
    • Jin‑A Choi
    • Da‑Young Pack
    • Ik‑Rae Lee
    • Min‑Gyu Kim
    • Sang‑Seop Han
    • Bo‑Yeon Kim
    • Sang‑Muk Oh
    • Kyung‑Bok Lee
    • Dong‑Jae Kim
    • Jong‑Hwan Park
  • View Affiliations

  • Published online on: November 23, 2015     https://doi.org/10.3892/mmr.2015.4602
  • Pages: 967-972
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Withaferin A (WA), a withanolide purified from Withania somnifera, has been known to exert anti-inflammatory effects. The present study sought to determine the effects of WA on Helicobacter (H.) pylori-mediated inflammation in the AGS gastric epithelial cell line. Cellular production of interleukin (IL)-8 and vascular endothelial growth factor (VEGF) was measured by ELISA. Western blot analysis was performed to determine the activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) as well as hypoxia-inducible factor 1α stabilization. Bacterial growth was also examined by measuring the optical density. Pre-treatment or co-treatment with WA efficiently reduced IL-8 production by AGS cells in response to H. pylori infection. H. pylori-induced activation of NF-κB, but not MAPKs, was also inhibited by pre-treatment of WA in the cells. However, WA did not affect VEGF production and HIF-1α stabilization induced by H. pylori in AGS cells. In addition, WA did not influence the growth of H. pylori, suggesting that the anti-inflammatory effect of WA was not due to any bactericidal effect. These findings indicate that WA is a potential preventive or therapeutic agent for H. pylori-mediated gastric inflammation.
View Figures
View References

Related Articles

Journal Cover

January-2016
Volume 13 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kim G, Kim TH, Kang MJ, Choi JA, Pack DY, Lee IR, Kim MG, Han SS, Kim BY, Oh SM, Oh SM, et al: Inhibitory effect of withaferin A on Helicobacter pylori‑induced IL‑8 production and NF‑κB activation in gastric epithelial cells. Mol Med Rep 13: 967-972, 2016.
APA
Kim, G., Kim, T., Kang, M., Choi, J., Pack, D., Lee, I. ... Park, J. (2016). Inhibitory effect of withaferin A on Helicobacter pylori‑induced IL‑8 production and NF‑κB activation in gastric epithelial cells. Molecular Medicine Reports, 13, 967-972. https://doi.org/10.3892/mmr.2015.4602
MLA
Kim, G., Kim, T., Kang, M., Choi, J., Pack, D., Lee, I., Kim, M., Han, S., Kim, B., Oh, S., Lee, K., Kim, D., Park, J."Inhibitory effect of withaferin A on Helicobacter pylori‑induced IL‑8 production and NF‑κB activation in gastric epithelial cells". Molecular Medicine Reports 13.1 (2016): 967-972.
Chicago
Kim, G., Kim, T., Kang, M., Choi, J., Pack, D., Lee, I., Kim, M., Han, S., Kim, B., Oh, S., Lee, K., Kim, D., Park, J."Inhibitory effect of withaferin A on Helicobacter pylori‑induced IL‑8 production and NF‑κB activation in gastric epithelial cells". Molecular Medicine Reports 13, no. 1 (2016): 967-972. https://doi.org/10.3892/mmr.2015.4602