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Article

Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2

  • Authors:
    • Xiaohong Tian
    • Jun Fan
    • Weijian Hou
    • Shuling Bai
    • Qiang Ao
    • Hao Tong
  • View Affiliations / Copyright

    Affiliations: Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, Liaoning 110122, P.R. China
  • Pages: 874-880
    |
    Published online on: November 26, 2015
       https://doi.org/10.3892/mmr.2015.4616
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Abstract

PIWIs have been shown to be abnormally expressed in a variety of cancers and may be important in the maintenance and invasion of cancer cells. The high expression of PIWIL2 contributed to the resistance effect of cisplatin in colon cancer cells, and the knockout of the PIWIL2 gene reduced the aggressive nature and malignant degree of colon cancer cells. Sodium orthovanadate (SOV) is a vanadium compound, and exhibited antineoplastic activity in certain types of human cancer cells, including lung, kidney and prostate cancer cells. However, its effects in human neuroblastoma (NB) cells have not yet been reported. The objective of this study was to investigate the effect of SOV on the apoptosis of NB cells and to explore how PIWIL2 is involved in the mechanism underlying this effect. In the present study, SH‑SY5Y cells were treated with SOV and the optimal concentration was determined for further assays. Cell apoptosis, cell count, viability, the cell cycle, and the expression of PIWIL2 mRNA and protein were then determined. The results showed that SOV could induce cell apoptosis, reduce the percentage of viable cells, induce accumulation of SH‑SY5Y cells at the G2/M and S phase of the cell cycle, and inhibit the expression of PIWIL2 and Bcl‑2 mRNA and protein. The results suggested that the underlying mechanisms may be, at least in part, due to SOV inhibiting the expression of PIWIL2. These findings demonstrated the effect of SOV and supported its further evaluation as a treatment for human NB.
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Copy and paste a formatted citation
Spandidos Publications style
Tian X, Fan J, Hou W, Bai S, Ao Q and Tong H: Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2. Mol Med Rep 13: 874-880, 2016.
APA
Tian, X., Fan, J., Hou, W., Bai, S., Ao, Q., & Tong, H. (2016). Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2. Molecular Medicine Reports, 13, 874-880. https://doi.org/10.3892/mmr.2015.4616
MLA
Tian, X., Fan, J., Hou, W., Bai, S., Ao, Q., Tong, H."Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2". Molecular Medicine Reports 13.1 (2016): 874-880.
Chicago
Tian, X., Fan, J., Hou, W., Bai, S., Ao, Q., Tong, H."Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2". Molecular Medicine Reports 13, no. 1 (2016): 874-880. https://doi.org/10.3892/mmr.2015.4616
Copy and paste a formatted citation
x
Spandidos Publications style
Tian X, Fan J, Hou W, Bai S, Ao Q and Tong H: Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2. Mol Med Rep 13: 874-880, 2016.
APA
Tian, X., Fan, J., Hou, W., Bai, S., Ao, Q., & Tong, H. (2016). Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2. Molecular Medicine Reports, 13, 874-880. https://doi.org/10.3892/mmr.2015.4616
MLA
Tian, X., Fan, J., Hou, W., Bai, S., Ao, Q., Tong, H."Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2". Molecular Medicine Reports 13.1 (2016): 874-880.
Chicago
Tian, X., Fan, J., Hou, W., Bai, S., Ao, Q., Tong, H."Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2". Molecular Medicine Reports 13, no. 1 (2016): 874-880. https://doi.org/10.3892/mmr.2015.4616
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