Fructus xanthii improves lipid homeostasis in the epididymal adipose tissue of rats fed a high-fat diet

Li,Xiumin; Yang,Mingxing; Li,Zhipeng; Xue,Mei; Shangguan,Zhaoshui; Ou,Zhimin; Liu,Ming; Liu,Suhuan; Yang,Shuyu; Li,Xuejun

doi:10.3892/mmr.2015.4628

Fructus xanthii improves lipid homeostasis in the epididymal adipose tissue of rats fed a high-fat diet

Authors:
- Xiumin Li
- Mingxing Yang
- Zhipeng Li
- Mei Xue
- Zhaoshui Shangguan
- Zhimin Ou
- Ming Liu
- Suhuan Liu
- Shuyu Yang
- Xuejun Li
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Affiliations: Xiamen Diabetes Institute, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China, Central Laboratory, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China
Published online on: November 30, 2015 https://doi.org/10.3892/mmr.2015.4628
Pages: 787-795
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

High fat diet (HFD)-induced obesity triggers common features of human metabolic syndrome in rats. Our previous study showed that Fructus xanthii (FX) attenuates HFD-induced hepatic steatosis. The present study was designed to investigate the effects of FX on lipid metabolism in epididymal fat (EF), and examine its underlying mechanisms. Aqueous extraction fractions of FX or vehicle were orally administered by gavage for 6 weeks to rats fed either a HFD or a normal chow diet (NCD). The levels of circulating free fatty acid (FFA) were determined in plasma, and the expression levels of lipid metabolism‑ and inflammation‑associated genes in the EF were measured using reverse transcription‑quantitative polymerase chain reaction analysis. The general morphology, size and number of adipocytes in the EF, and the levels of macrophage infiltration were evaluated using hematoxylin and eosin staining or immunohistochemical staining. FX decreased circulating levels of FFA, increased the expression levels of sterol‑regulatory‑element‑binding protein‑1c, FAS, acetyl coenzyme A carboxylase, diacylglycerol acyltransferase and lipoprotein lipase lipogenic genes in the EF. FX increased the numbers of adipocytes in the EF, and featured a shift towards smaller adipocyte size. Compared with the vehicle‑treated rats, positive staining of F4/80 was more dispersed in the FX‑treated rats, and the percentage of F4/80 positive cells was significantly decreased. FX attenuated HFD‑induced lipid dyshomeostasis in the epididymal adipose tissue.

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1	Handschin C and Spiegelman BM: The role of exercise and PGC1 alpha in inflammation and chronic disease. Nature. 454:463–469. 2008. View Article : Google Scholar : PubMed/NCBI
2	Duval C, Thissen U, Keshtkar S, Accart B, Stienstra R, Boekschoten MV, Roskams T, Kersten S and Müller M: Adipose tissue dysfunction signals progression of hepatic steatosis towards nonalcoholic steatohepatitis in C57Bl/6 mice. Diabetes. 59:3181–3191. 2010. View Article : Google Scholar : PubMed/NCBI
3	Postic C and Girard J: The role of the lipogenic pathway in the development of hepatic steatosis. Diabetes Metab. 34(6 Pt 2): 643–648. 2008. View Article : Google Scholar
4	Seo JB, Moo HM, Kim WS, Lee YS, Jeong HW, Yoo EJ, Ham J, Kang H, Park MG, Steffensen KR, et al: Activated liver X receptors stimulate adipocyte differentiation through induction of peroxisome proliferator-activated receptor gamma expression. Mol Cell Biol. 24:3430–3444. 2004. View Article : Google Scholar : PubMed/NCBI
5	Marcelin G and Chua S Jr: Contributions of adipocyte lipid metabolism to body fat content and implications for the treatment of obesity. Curr Opin Pharmacol. 10:588–593. 2010. View Article : Google Scholar : PubMed/NCBI
6	Takeuchi K and Reue K: Biochemistry, physiology and genetics of GPAT, AGPAT and lipin enzymes in triglyceride synthesis. Am J Physiol Endocrinol Metab. 296:E1195–E1209. 2009. View Article : Google Scholar : PubMed/NCBI
7	Gimeno RE and Cao J: Thematic review series: Glycerolipids. Mammalian glycerol-3-phosphate acyltransferases: New genes for an old activity. J Lipid Res. 49:2079–2088. 2008. View Article : Google Scholar : PubMed/NCBI
8	Shindou H, Hishikawa D, Harayama T, Yuki K and Shimizu T: Recent progress on acyl-CoA: Lysophospholipid acyltransferase research. J Lipid Res. 50(Suppl): S46–S51. 2009. View Article : Google Scholar :
9	Yen CL, Stone SJ, Koliwad S, Harris C and Farese RV Jr: Thematic review series: Glycerolipids. DGAT enzymes and triacylglycerol biosynthesis. J Lipid Res. 49:2283–2301. 2008. View Article : Google Scholar : PubMed/NCBI
10	Gong J, Sun Z, Wu L, Xu W, Schieber N, Xu D, Shui G, Yang H, Parton RG and Li P: Fsp27 promotes lipid droplet growth by lipid exchange and transfer at lipid droplet contact sites. J Cell Biol. 195:953–963. 2011. View Article : Google Scholar : PubMed/NCBI
11	Rodriguez-Cuenca S, Carobbio S, Velagapudi VR, Barbarroja N, Moreno-Navarrete JM, Tinahones FJ, Fernandez-Real JM, Orešic M and Vidal-Puig A: Peroxisome proliferator-activated receptor γ-dependent regulation of lipolytic nodes and metabolic flexibility. Mol Cell Biol. 32:1555–1565. 2012. View Article : Google Scholar : PubMed/NCBI
12	Wellen KE and Hotamisligil GS: Obesity-induced inflammatory changes in adipose tissue. J Clin Invest. 112:1785–1788. 2003. View Article : Google Scholar : PubMed/NCBI
13	Sartipy P and Loskutoff DJ: Monocyte chemoattractant protein 1 in obesity and insulin resistance. Proc Natl Acad Sci USA. 100:7265–7270. 2003. View Article : Google Scholar : PubMed/NCBI
14	Kopelman PG: Obesity as a medical problem. Nature. 404:635–643. 2000.PubMed/NCBI
15	Wanless LR and Lentz JS: Fatty liver hepatitis and obesity: An autopsy study with analysis of risk factors. Hepatology. 12:1106–1110. 1990. View Article : Google Scholar : PubMed/NCBI
16	Ma P and Li H: The research development of Fructus xanthii. Chinese Herbal Medicine. 30:634–636. 1999.
17	Huang MH, Wang BS, Chiu CS, Amagaya S, Hsieh WT, Huang SS, Shie PH and Huang GJ: Antioxidant, antinociceptive and anti-inflammatory activities of Xanthii Fructus extract. J Ethnopharmacol. 135:545–552. 2011. View Article : Google Scholar : PubMed/NCBI
18	Song MY, Kim EK, Lee HJ, Park JW, Ryu DG, Kwon KB and Park BH: Fructus Xanthii extract protects against cytokine-induced damage in pancreatic beta-cells through suppression of NF-kappaB activation. Int J Mol Med. 23:547–553. 2009.PubMed/NCBI
19	Li XM, Li ZP, Xue M, Ou ZM, Liu M, Yang M, Liu S, Yang S and Li X: Fructus Xanthii attenuates hepatic steatosis in rats fed on high-fat diet. Plos one. 8:e614992013. View Article : Google Scholar : PubMed/NCBI
20	Kato K, Takamura T, Takeshita Y, Ryu Y, Misu H, Ota T, Tokuyama K, Nagasaka S, Matsuhisa M, Matsui O and Kaneko S: Ectopic fat accumulation and distant organ-specific insulin resistance in Japanese people with nonalcoholic fatty liver disease. PLoS One. 9:e921702014. View Article : Google Scholar : PubMed/NCBI
21	Lument CN and Saltiel AR: Inflammatory links between obesity and metabolic disease. J Clin Invest. 121:2111–2117. 2011. View Article : Google Scholar
22	Kim JY, van de Wall E, Laplante M, Azzara A, Trujillo ME, Hofmann SM, Schraw T, Durand JL, Li H, Li G, et al: Obesity-associated improvements in metabolic profile through expansion of adipose tissue. J Clin Invest. 117:2621–2637. 2007. View Article : Google Scholar : PubMed/NCBI
23	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2[−Delta Delta C(T)] Method. Methods. 25:402–408. 2001. View Article : Google Scholar
24	Ginsberg HN, Zhang YL and Hernandez-Ono A: Metabolic syndrome: Focus on dyslipidemia. Obesity (Silver Spring). 14(Suppl 1): S41–S49. 2006. View Article : Google Scholar
25	Sun X, Tang Y, Tan X, Li Q, Zhong W, Sun X, Jia W, McClain CJ and Zhou Z: Activation of peroxisome proliferator-activated receptor-γ by rosiglitazone improves lipid homeostasis at the adipose tissue-liver axis in ethanol-fed mice. Am J Physiol Gastrointest Liver Physiol. 302:G548–G557. 2012. View Article : Google Scholar
26	Khan T, Muise ES, Iyengar P, Wang ZV, Chandalia M, Abate N, Zhang BB, Bonaldo P, Chua S and Scherer PE: Metabolic dysregulation and adipose tissue fibrosis: Role of collage VI. Mol Cell Biol. 29:1575–1591. 2009. View Article : Google Scholar :
27	Hofbauer KG: Molecular patheays to obesity. Int J Obes Relat Metab Disord. 26(Suppl 2): S18–S27. 2002. View Article : Google Scholar
28	Alberdi G, Rodríguez V, Miranda J, Macarulla M, Arias N, Andrés-Lacueva C and Portillo MP: Changes in white adipose tissue metabolism induced by resveratrol in rats. Nutr Metab (Lond). 8:292011. View Article : Google Scholar
29	Wang M, Grayburn P, Chen S, Ravazzola M, Orci L and Unger RH: Adipogenic capacity and the susceptibility to type 2 diabetes and metabolic syndrome. Proc Natl Acad Sci USA. 105:6139–6144. 2008. View Article : Google Scholar : PubMed/NCBI
30	Letexier D, Pinteur C, Large V, Fréring V and Beylot M: Comparison of the expression and activity of the lipogenic pathway in human and rat adipose tissue. J Lipid Res. 44:2127–2134. 2003. View Article : Google Scholar : PubMed/NCBI
31	Strissel KJ, Stancheva Z, Miyoshi H, Perfield JW II, DeFuria J, Jlick Z, Greenberg AS and Obin MS: Adipocyte death, adipose tissue remodeling and obesity complications. Diabetes. 56:2910–2918. 2007. View Article : Google Scholar : PubMed/NCBI
32	Zhang HR and Zhang CH: Adipose 'talk' to distant organs to regulate insulin sensitivity and vascular function. Obesity (Silver Spring). 18:2071–2076. 2010. View Article : Google Scholar
33	Large V, Peroni O, Letexier D, Ray H and Beylot M: Metabolism of lipids in human white adipocyte. Diabetes Metab. 30:294–309. 2004. View Article : Google Scholar : PubMed/NCBI