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Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease

  • Authors:
    • Xin Wang
    • Shiqi Wang
    • Xianqing Jin
    • Ning Wang
    • Yuanyuan Luo
    • Yinping Teng
  • View Affiliations / Copyright

    Affiliations: Tumour Laboratory of Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 641-650
    |
    Published online on: December 1, 2015
       https://doi.org/10.3892/mmr.2015.4633
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Abstract

The present study investigated a genome microarray of colorectal lesions (spasm segments) in children with Hirschsprung's disease (HSCR), and analyzed the results. In addition, the present study screened for differentially expressed genes in children with HSCR. Microarray technology was used to examine the human gene expression profiles of the colorectal lesions (spasm segments) of six children with HSCR, and three normal colon tissue samples. The data were analyzed be determining P‑values of significance and absolute fold changes. Preliminary screening was performed to identify genes exhibiting significant differential expression in children with HSCR, and these target genes were analyzed in subsequent verification and analytical investigations. Of >20,000 detected human genes, the preliminary screenings demonstrated that 3,850 genes were differentially expressed and upregulated, with P<0.05 and >2‑fold absolute changes in expression. In addition, 645 differentially expressed genes with P<0.05 and >2‑fold absolute changes were downregulated. Of the upregulated genes, 118 were involved in classic signaling pathways, compared with 11 of the downregulated genes (P<0.001; absolute fold change >2‑fold). HSCR etiology is complex and often involves multiple gene changes. Microarray technology can produce large quantities of gene expression data simultaneously, and analyzing this data using various techniques may provide a fast and efficient method for identifying novel gene targets and for investigating the mechanisms underlying HSCR pathogenesis.
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Spandidos Publications style
Wang X, Wang S, Jin X, Wang N, Luo Y and Teng Y: Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease. Mol Med Rep 13: 641-650, 2016.
APA
Wang, X., Wang, S., Jin, X., Wang, N., Luo, Y., & Teng, Y. (2016). Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease. Molecular Medicine Reports, 13, 641-650. https://doi.org/10.3892/mmr.2015.4633
MLA
Wang, X., Wang, S., Jin, X., Wang, N., Luo, Y., Teng, Y."Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease". Molecular Medicine Reports 13.1 (2016): 641-650.
Chicago
Wang, X., Wang, S., Jin, X., Wang, N., Luo, Y., Teng, Y."Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease". Molecular Medicine Reports 13, no. 1 (2016): 641-650. https://doi.org/10.3892/mmr.2015.4633
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Wang S, Jin X, Wang N, Luo Y and Teng Y: Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease. Mol Med Rep 13: 641-650, 2016.
APA
Wang, X., Wang, S., Jin, X., Wang, N., Luo, Y., & Teng, Y. (2016). Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease. Molecular Medicine Reports, 13, 641-650. https://doi.org/10.3892/mmr.2015.4633
MLA
Wang, X., Wang, S., Jin, X., Wang, N., Luo, Y., Teng, Y."Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease". Molecular Medicine Reports 13.1 (2016): 641-650.
Chicago
Wang, X., Wang, S., Jin, X., Wang, N., Luo, Y., Teng, Y."Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease". Molecular Medicine Reports 13, no. 1 (2016): 641-650. https://doi.org/10.3892/mmr.2015.4633
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