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Article

MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase

  • Authors:
    • Chun‑Yan Zeng
    • Yi‑Shan Zhan
    • Jun Huang
    • You‑Xiang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 1297-1303
    |
    Published online on: December 4, 2015
       https://doi.org/10.3892/mmr.2015.4643
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Abstract

Previous studies have demonstrated that microRNA (miRNA) are essential in tumor development and invasion. The close association between focal adhesion kinase (FAK) and colon cancer (CC) has been previously reported. miRNA‑7 (miR‑7) inhibits the translation of FAK protein. Therefore, the present study aimed to assess the underlying molecular mechanism of miR‑7 in human CC cell lines, to provide a novel therapeutic biomarker of CC in the future. The present study detected the expression of miR‑7 in 60 CC tissues by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The association between the expression of miR‑7 and clinical pathological factors was analyzed. Overexpression/underexpression of miR‑7 were established by transfecting miR‑7mimics/inhibitors into HCT‑8 and Caco‑2 cells. The transfected CC cell lines were used in cell viability and scratching assays. The regulation of FAK by miR‑7 was analyzed by western blotting and RT‑qPCR. It was demonstrated that the expression of miR‑7 negatively correlated with lymph node metastasis and tumor node metastasis staging in CC (P<0.05). Inhibition of miR‑7 led to an accelerated ability of proliferation and migration in CC cell lines. Additionally, overexpression of miR‑7 inhibited the proliferation and migration of CC cells. In addition, it was also observed that miR‑7 regulated the proliferation and migration of CC by regulating the protein expression of FAK, therefore, regulating the expression of matrix metalloproteinase (MMP)‑2 and MMP‑9. miR‑7 inhibited the proliferation and migration of CC cells by regulating FAK. These findings suggested that miR‑7 may be a novel therapeutic target for CC.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng CY, Zhan YS, Huang J and Chen YX: MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase. Mol Med Rep 13: 1297-1303, 2016.
APA
Zeng, C., Zhan, Y., Huang, J., & Chen, Y. (2016). MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase. Molecular Medicine Reports, 13, 1297-1303. https://doi.org/10.3892/mmr.2015.4643
MLA
Zeng, C., Zhan, Y., Huang, J., Chen, Y."MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase". Molecular Medicine Reports 13.2 (2016): 1297-1303.
Chicago
Zeng, C., Zhan, Y., Huang, J., Chen, Y."MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase". Molecular Medicine Reports 13, no. 2 (2016): 1297-1303. https://doi.org/10.3892/mmr.2015.4643
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng CY, Zhan YS, Huang J and Chen YX: MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase. Mol Med Rep 13: 1297-1303, 2016.
APA
Zeng, C., Zhan, Y., Huang, J., & Chen, Y. (2016). MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase. Molecular Medicine Reports, 13, 1297-1303. https://doi.org/10.3892/mmr.2015.4643
MLA
Zeng, C., Zhan, Y., Huang, J., Chen, Y."MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase". Molecular Medicine Reports 13.2 (2016): 1297-1303.
Chicago
Zeng, C., Zhan, Y., Huang, J., Chen, Y."MicroRNA‑7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase". Molecular Medicine Reports 13, no. 2 (2016): 1297-1303. https://doi.org/10.3892/mmr.2015.4643
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