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Article Open Access

N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration

  • Authors:
    • Jie Cai
    • Mei Wang
    • Mengchu Zhu
    • Qiang Zhang
    • Xu Zhang
    • Yongmin Yan
    • Hui Qian
    • Wenrong Xu
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, The Affiliated Hospital, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China
    Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1083-1090
    |
    Published online on: December 7, 2015
       https://doi.org/10.3892/mmr.2015.4650
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Abstract

Chronic inflammation has a decisive role in tumorigenesis, particularly in gastric carcinogenesis. The CC chemokine ligand 2 (CCL2), an important inflammatory cytokine, is involved in the initiation, development and progression of various types of cancer. However, the role of CCL2 in gastric cancer remains to be elucidated. The present study demonstrated that recombinant CCL2 stimulation caused no effect on the morphology, proliferation and migration of human GES‑1 gastric mucosa epithelial cells, in which the protein expression of CC‑chemokine receptor 2 (CCR2) was markedly low. However, the expression of CCR2 was significantly upregulated in the GES‑1 cells following pretreatment with the chemical carcinogen, N‑methyl‑N‑nitro‑N'‑nitrosoguanidine (MNNG), for 12 h or transformed with MNNG (MC cells). The present study used CCL2 to stimulate MNNG pretreated GES‑1 cells and MC cells, and demonstrated that CCL2 clearly promoted their migration and the epithelial‑mesenchymal transition (EMT). However, no effect was observed on the proliferative ability of the cells. Taken together, these findings suggested that the CCL2/CCR2 chemokine signaling may regulate the EMT in gastric epithelial cells and resulted in gastric carcinogenesis in response to the intake of the carcinogen, MNNG.
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Copy and paste a formatted citation
Spandidos Publications style
Cai J, Wang M, Zhu M, Zhang Q, Zhang X, Yan Y, Qian H and Xu W: N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration. Mol Med Rep 13: 1083-1090, 2016.
APA
Cai, J., Wang, M., Zhu, M., Zhang, Q., Zhang, X., Yan, Y. ... Xu, W. (2016). N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration. Molecular Medicine Reports, 13, 1083-1090. https://doi.org/10.3892/mmr.2015.4650
MLA
Cai, J., Wang, M., Zhu, M., Zhang, Q., Zhang, X., Yan, Y., Qian, H., Xu, W."N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration". Molecular Medicine Reports 13.2 (2016): 1083-1090.
Chicago
Cai, J., Wang, M., Zhu, M., Zhang, Q., Zhang, X., Yan, Y., Qian, H., Xu, W."N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration". Molecular Medicine Reports 13, no. 2 (2016): 1083-1090. https://doi.org/10.3892/mmr.2015.4650
Copy and paste a formatted citation
x
Spandidos Publications style
Cai J, Wang M, Zhu M, Zhang Q, Zhang X, Yan Y, Qian H and Xu W: N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration. Mol Med Rep 13: 1083-1090, 2016.
APA
Cai, J., Wang, M., Zhu, M., Zhang, Q., Zhang, X., Yan, Y. ... Xu, W. (2016). N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration. Molecular Medicine Reports, 13, 1083-1090. https://doi.org/10.3892/mmr.2015.4650
MLA
Cai, J., Wang, M., Zhu, M., Zhang, Q., Zhang, X., Yan, Y., Qian, H., Xu, W."N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration". Molecular Medicine Reports 13.2 (2016): 1083-1090.
Chicago
Cai, J., Wang, M., Zhu, M., Zhang, Q., Zhang, X., Yan, Y., Qian, H., Xu, W."N‑methyl‑N‑nitro‑N'‑nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2‑mediated migration". Molecular Medicine Reports 13, no. 2 (2016): 1083-1090. https://doi.org/10.3892/mmr.2015.4650
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