Induction of apoptosis in MCF‑7 human breast cancer cells by Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelium)

  • Authors:
    • Tae Hwan Kim
    • Ju Sung Kim
    • Zoo Haye Kim
    • Ren Bin Huang
    • Young Lye Chae
    • Ren Sheng Wang
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  • Published online on: December 7, 2015     https://doi.org/10.3892/mmr.2015.4655
  • Pages: 1243-1249
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Abstract

Khz (fusion of Ganoderma lucidum and Polyporus umbellatus), isolated from the mycelia of G. lucidum and P. umbellatus, exerts anti‑proliferative effects against malignant cells; however, its activity against human breast cancer cells remains to be elucidated. In the present study, cell proliferation was assessed using a 3-(4,5‑dimethylthiazol‑2‑yl)-2,5‑diphenyltetrazolium bromide assay, and poptosis was examined using annexin V‑propidium iodide staining and flow cytometry. The activation of caspases 7, 8 and 9 were detected in the Khz‑treated cells using western blotting. The results demonstrated that Khz increased the intracellular calcium concentration and induced the production of reactive oxygen species in MCF‑7 breast cancer cells, as determined using flow cytometry. The results also demonstrated that Khz inhibited cell proliferation and induced apoptosis in the MCF‑7 cells. In addition, the mechanism by which Khz induces apoptosis in cancer cells was investigated. Khz induced apoptosis preferentially in transformed cells, with a minimal effect on non‑transformed cells, suggesting its potential as an anticancer therapeutic agent. Oxidative stress is associated with apoptotic and non‑apoptotic cell death, although pro‑oxidative conditions are not a pre‑requisite for apoptosis. Assessment of the activation status of caspases 7, 8 and 9 revealed that the levels of cleaved caspases were significantly increased in the cells treated with Khz. It is widely accepted that calcium signaling is important in apoptosis, and the present study observed an increase in [Ca2+]i in response to Khz treatment. The anti‑proliferative and pro‑apoptotic effects of Khz suggest that this extract may be developed as a potential anticancer agent.
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February-2016
Volume 13 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kim TH, Kim JS, Kim ZH, Huang RB, Chae YL and Wang RS: Induction of apoptosis in MCF‑7 human breast cancer cells by Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelium). Mol Med Rep 13: 1243-1249, 2016
APA
Kim, T.H., Kim, J.S., Kim, Z.H., Huang, R.B., Chae, Y.L., & Wang, R.S. (2016). Induction of apoptosis in MCF‑7 human breast cancer cells by Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelium). Molecular Medicine Reports, 13, 1243-1249. https://doi.org/10.3892/mmr.2015.4655
MLA
Kim, T. H., Kim, J. S., Kim, Z. H., Huang, R. B., Chae, Y. L., Wang, R. S."Induction of apoptosis in MCF‑7 human breast cancer cells by Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelium)". Molecular Medicine Reports 13.2 (2016): 1243-1249.
Chicago
Kim, T. H., Kim, J. S., Kim, Z. H., Huang, R. B., Chae, Y. L., Wang, R. S."Induction of apoptosis in MCF‑7 human breast cancer cells by Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelium)". Molecular Medicine Reports 13, no. 2 (2016): 1243-1249. https://doi.org/10.3892/mmr.2015.4655