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Article

MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis

  • Authors:
    • Yifan Li
    • Duqun Chen
    • Zhengming Su
    • Yuchi Li
    • Jiaju Liu
    • Lu Jin
    • Min Shi
    • Zhimao Jiang
    • Zhengyu Qi
    • Yaoting Gui
    • Shangqi Yang
    • Xiangming Mao
    • Xionghui Wu
    • Yongqing Lai
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Department of Urology, The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China
  • Pages: 1420-1426
    |
    Published online on: December 8, 2015
       https://doi.org/10.3892/mmr.2015.4656
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Abstract

Kidney cancer is the 14th most common cancer in the world and its prognosis remains poor due to difficult early detection and treatment. Therefore, the identification of biomarkers for early‑stage renal cell carcinoma (RCC) is important. MicroRNA‑106b (miR‑106b) has been described as an oncogene in several types of human cancer. Previous microarray studies have suggested that miR‑106b was significantly upregulated in RCC tissues compared with paired normal kidney tissues and may be a promising biomarker for the prediction of early metastasis following nephrectomy. The present study aimed to determine the expression and function of miR‑106b in RCC. The expression of miR‑106b in RCC tissues and cells, and in paired normal tissues and cells was determined by reverse transcription quantitative polymerase chain reaction, based on the previous sequencing results of miRNAs. Furthermore, a wound scratch assay, MTT assay and flow cytometry were performed to examine the functions of miR‑106b on cell migration, proliferation and apoptosis. The results demonstrated that miR‑106b was upregulated in RCC tissues and cell lines compared with control normal tissues and cell lines. Downregulation of miR‑106b with a synthesized inhibitor suppressed cell migration and proliferation and induced renal cancer cell apoptosis, suggesting that miR‑106b can be characterized as an oncogene in RCC. To the best of our knowledge, the present study was the first to reveal that miR‑106b is upregulated and affects cellular migration, proliferation and apoptosis in RCC. Further studies are required to examine the role and target genes of miR‑106b in RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Chen D, Su Z, Li Y, Liu J, Jin L, Shi M, Jiang Z, Qi Z, Gui Y, Gui Y, et al: MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis. Mol Med Rep 13: 1420-1426, 2016.
APA
Li, Y., Chen, D., Su, Z., Li, Y., Liu, J., Jin, L. ... Lai, Y. (2016). MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis. Molecular Medicine Reports, 13, 1420-1426. https://doi.org/10.3892/mmr.2015.4656
MLA
Li, Y., Chen, D., Su, Z., Li, Y., Liu, J., Jin, L., Shi, M., Jiang, Z., Qi, Z., Gui, Y., Yang, S., Mao, X., Wu, X., Lai, Y."MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis". Molecular Medicine Reports 13.2 (2016): 1420-1426.
Chicago
Li, Y., Chen, D., Su, Z., Li, Y., Liu, J., Jin, L., Shi, M., Jiang, Z., Qi, Z., Gui, Y., Yang, S., Mao, X., Wu, X., Lai, Y."MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis". Molecular Medicine Reports 13, no. 2 (2016): 1420-1426. https://doi.org/10.3892/mmr.2015.4656
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Chen D, Su Z, Li Y, Liu J, Jin L, Shi M, Jiang Z, Qi Z, Gui Y, Gui Y, et al: MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis. Mol Med Rep 13: 1420-1426, 2016.
APA
Li, Y., Chen, D., Su, Z., Li, Y., Liu, J., Jin, L. ... Lai, Y. (2016). MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis. Molecular Medicine Reports, 13, 1420-1426. https://doi.org/10.3892/mmr.2015.4656
MLA
Li, Y., Chen, D., Su, Z., Li, Y., Liu, J., Jin, L., Shi, M., Jiang, Z., Qi, Z., Gui, Y., Yang, S., Mao, X., Wu, X., Lai, Y."MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis". Molecular Medicine Reports 13.2 (2016): 1420-1426.
Chicago
Li, Y., Chen, D., Su, Z., Li, Y., Liu, J., Jin, L., Shi, M., Jiang, Z., Qi, Z., Gui, Y., Yang, S., Mao, X., Wu, X., Lai, Y."MicroRNA‑106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis". Molecular Medicine Reports 13, no. 2 (2016): 1420-1426. https://doi.org/10.3892/mmr.2015.4656
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