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Article

Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2

  • Authors:
    • Chin‑Cheng Su
  • View Affiliations / Copyright

    Affiliations: Tumor Research Center of Integrative Medicine, Changhua Christian Hospital, Changhua, Changhua 50006, Taiwan, R.O.C.
  • Pages: 1263-1268
    |
    Published online on: December 8, 2015
       https://doi.org/10.3892/mmr.2015.4658
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Abstract

During progression of gastric cancer, degradation of the extracellular matrix by matrix metalloproteinases (MMPs) has been associated with poor prognosis. Tanshinone IIA (Tan‑IIA) exerts antitumor activity in a variety of human cancer cells. It is extracted from Danshen (Salviae miltiorrhizae radix), and induces apoptosis and inhibits the proliferation of gastric cancer cells. However, the molecular mechanisms underlying the inhibition of migration in gastric cancer by Tan‑IIA have not been fully elucidated. In the present study, AGS cell migration ability was evaluated using a wound‑healing assay. The protein expression levels of nuclear factor (NF)‑κB‑p65, cyclooxygenase (COX)‑2, MMP‑2, ‑7, and ‑9 and β‑actin in AGS cells were measured by western blotting. The results demonstrated that AGS cells treated with Tan‑IIA exhibit decreased protein expression levels of NF‑κB‑p65, COX‑2, and MMP‑2, ‑7 and ‑9. The results also indicate that Tan‑IIA inhibits migration ability in a dose‑ and time‑dependent manner. These findings demonstrate that Tan‑IIA inhibits the migration ability of AGS human gastric cancer cells and that decreasing the protein expression of NF‑κB‑p65, COX‑2, and MMP‑2, ‑7 and ‑9 may be an underlying molecular mechanism.
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Copy and paste a formatted citation
Spandidos Publications style
Su CC: Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2. Mol Med Rep 13: 1263-1268, 2016.
APA
Su, C. (2016). Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2. Molecular Medicine Reports, 13, 1263-1268. https://doi.org/10.3892/mmr.2015.4658
MLA
Su, C."Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2". Molecular Medicine Reports 13.2 (2016): 1263-1268.
Chicago
Su, C."Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2". Molecular Medicine Reports 13, no. 2 (2016): 1263-1268. https://doi.org/10.3892/mmr.2015.4658
Copy and paste a formatted citation
x
Spandidos Publications style
Su CC: Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2. Mol Med Rep 13: 1263-1268, 2016.
APA
Su, C. (2016). Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2. Molecular Medicine Reports, 13, 1263-1268. https://doi.org/10.3892/mmr.2015.4658
MLA
Su, C."Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2". Molecular Medicine Reports 13.2 (2016): 1263-1268.
Chicago
Su, C."Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB‑p65 and cyclooxygenase‑2". Molecular Medicine Reports 13, no. 2 (2016): 1263-1268. https://doi.org/10.3892/mmr.2015.4658
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