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CRMP‑5 interacts with actin to regulate neurite outgrowth

  • Authors:
    • Xiaobing Gong
    • Minghui Tan
    • Yuan Gao
    • Keen Chen
    • Guoqing Guo
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Department of Anatomy, Medical College of Jinan University, Guangzhou, Guangdong 510630, P.R. China
    Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1179-1185
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    Published online on: December 9, 2015
       https://doi.org/10.3892/mmr.2015.4662
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Abstract

CRMP family proteins (CRMPs) are abundantly expressed in the developing nervous system mediating growth cone guidance, neuronal polarity and axon elongation. CRMP‑5 has been indicated to serve a critical role in neurite outgrowth. However, the detailed mechanisms of how CRMP‑5 regulates neurite outgrowth remain unclear. In the current study, co‑immunoprecipitation was used to identify the fact that CRMP‑5 interacted with the actin and tubulin cytoskeleton networks in the growth cones of developing hippocampal neurons. CRMP‑5 exhibited increased affinity towards actin when compared with microtubules. Immunocytochemistry was used to identify the fact that CRMP‑5 colocalized with actin predominantly in the C‑domain and T‑zone in growth cones. In addition, genetic inhibition of CRMP‑5 by siRNA suppressed the expression of actin, growth cone development and neurite outgrowth. Overexpression of CRMP‑5 promoted the interaction with actin, growth cone development and hippocampal neurite outgrowth. Taken together, these data suggest that CRMP‑5 is able to interact with the actin cytoskeleton network in the growth cone and affect growth cone development and neurite outgrowth via this interaction in developing hippocampal neurons.
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Copy and paste a formatted citation
Spandidos Publications style
Gong X, Tan M, Gao Y, Chen K and Guo G: CRMP‑5 interacts with actin to regulate neurite outgrowth. Mol Med Rep 13: 1179-1185, 2016.
APA
Gong, X., Tan, M., Gao, Y., Chen, K., & Guo, G. (2016). CRMP‑5 interacts with actin to regulate neurite outgrowth. Molecular Medicine Reports, 13, 1179-1185. https://doi.org/10.3892/mmr.2015.4662
MLA
Gong, X., Tan, M., Gao, Y., Chen, K., Guo, G."CRMP‑5 interacts with actin to regulate neurite outgrowth". Molecular Medicine Reports 13.2 (2016): 1179-1185.
Chicago
Gong, X., Tan, M., Gao, Y., Chen, K., Guo, G."CRMP‑5 interacts with actin to regulate neurite outgrowth". Molecular Medicine Reports 13, no. 2 (2016): 1179-1185. https://doi.org/10.3892/mmr.2015.4662
Copy and paste a formatted citation
x
Spandidos Publications style
Gong X, Tan M, Gao Y, Chen K and Guo G: CRMP‑5 interacts with actin to regulate neurite outgrowth. Mol Med Rep 13: 1179-1185, 2016.
APA
Gong, X., Tan, M., Gao, Y., Chen, K., & Guo, G. (2016). CRMP‑5 interacts with actin to regulate neurite outgrowth. Molecular Medicine Reports, 13, 1179-1185. https://doi.org/10.3892/mmr.2015.4662
MLA
Gong, X., Tan, M., Gao, Y., Chen, K., Guo, G."CRMP‑5 interacts with actin to regulate neurite outgrowth". Molecular Medicine Reports 13.2 (2016): 1179-1185.
Chicago
Gong, X., Tan, M., Gao, Y., Chen, K., Guo, G."CRMP‑5 interacts with actin to regulate neurite outgrowth". Molecular Medicine Reports 13, no. 2 (2016): 1179-1185. https://doi.org/10.3892/mmr.2015.4662
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