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Article

Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells

  • Authors:
    • Feng Yan
    • Jin He
    • Li Tang
    • Yi Kong
    • Yuhua Shi
    • Suihua Chen
    • Zhenping Huang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, P.R. China, Department of Neurosurgical Laboratory, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, P.R. China, Department of Ophthalmology, No. 97 Hospital, Xuzhou, Jiangsu 221004, P.R. China
  • Pages: 1367-1372
    |
    Published online on: December 9, 2015
       https://doi.org/10.3892/mmr.2015.4666
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Abstract

The present study investigated the underlying mechanism of the induction of regulatory T cells (Tregs) by retinal pigment epithelial (RPE) cells and the characteristics of these Tregs. Human RPE cells were cultured in the presence or absence of transforming growth factor‑β 2 (TGF‑β2), and reverse-transcription quantitative PCR was performed to determine the mRNA expression of indoleamine 2,3‑dioxygenase (IDO) and nuclear factor erythroid 2‑related factor (Nrf2). Supernatants of RPE cell cultures were added to CD4+ T cells to induce Tregs. The RPE‑induced Tregs were purified by two‑step magnetic cell sorting. The natural Tregs were isolated from the peripheral blood mononuclear cells of healthy volunteers. Purified CD4+ CD25- T cells (2x105/well) were cultured alone or with Tregs (various densities, natural or RPE‑induced). The proliferation of CD4+ CD25- T cells was determined by 3H‑thymidine incorporation. After 24 h of stimulation with TGF‑β2, the mRNA expression of IDO in RPE cells was upregulated. The highest level of IDO mRNA expression was reached after 72 h of stimulation with TGF‑β2. However, the Nrf2 mRNA expression was slightly decreased after 24 h of stimulation with TGF‑β2 and significantly increased after 48‑72 h of TGF‑β2 stimulation. Increased levels of CD25 expression were observed on CD4+ T cells exposed to supernatants of RPE cell cultures treated with TGF‑β2 and recombinant interleukin‑2. The RPE‑induced Tregs were more effective at suppressing the proliferation of CD4+ CD25- T cells compared with native Tregs. These findings suggested that IDO may be a signaling protein in RPE cells which is implicated in the induction of Tregs. RPE‑induced Tregs have the potential to be applied for immunotherapy for ocular inflammatory diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Yan F, He J, Tang L, Kong Y, Shi Y, Chen S and Huang Z: Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells. Mol Med Rep 13: 1367-1372, 2016.
APA
Yan, F., He, J., Tang, L., Kong, Y., Shi, Y., Chen, S., & Huang, Z. (2016). Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells. Molecular Medicine Reports, 13, 1367-1372. https://doi.org/10.3892/mmr.2015.4666
MLA
Yan, F., He, J., Tang, L., Kong, Y., Shi, Y., Chen, S., Huang, Z."Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells". Molecular Medicine Reports 13.2 (2016): 1367-1372.
Chicago
Yan, F., He, J., Tang, L., Kong, Y., Shi, Y., Chen, S., Huang, Z."Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells". Molecular Medicine Reports 13, no. 2 (2016): 1367-1372. https://doi.org/10.3892/mmr.2015.4666
Copy and paste a formatted citation
x
Spandidos Publications style
Yan F, He J, Tang L, Kong Y, Shi Y, Chen S and Huang Z: Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells. Mol Med Rep 13: 1367-1372, 2016.
APA
Yan, F., He, J., Tang, L., Kong, Y., Shi, Y., Chen, S., & Huang, Z. (2016). Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells. Molecular Medicine Reports, 13, 1367-1372. https://doi.org/10.3892/mmr.2015.4666
MLA
Yan, F., He, J., Tang, L., Kong, Y., Shi, Y., Chen, S., Huang, Z."Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells". Molecular Medicine Reports 13.2 (2016): 1367-1372.
Chicago
Yan, F., He, J., Tang, L., Kong, Y., Shi, Y., Chen, S., Huang, Z."Transforming growth factor‑β2 increases the capacity of retinal pigment epithelial cells to induce the generation of regulatory T cells". Molecular Medicine Reports 13, no. 2 (2016): 1367-1372. https://doi.org/10.3892/mmr.2015.4666
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