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Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling

  • Authors:
    • Xingyu Ze
    • Jidong Jia
    • Xinmin Li
    • Hong You
    • Xinyan Zhao
    • Dong Zhang
    • Baoen Wang
  • View Affiliations / Copyright

    Affiliations: Liver Disease Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China, Beijing Institute of Integrated Traditional and Western Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China
    Copyright: © Ze et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1501-1508
    |
    Published online on: December 18, 2015
       https://doi.org/10.3892/mmr.2015.4696
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Abstract

Tanshinone IIA (TSA) is a widely used traditional Chinese medicine, which has been demonstrated to protect damaged liver cells and is currently administered in the treatment of liver fibrosis. Liver precursor cells, also termed oval cells, are key in the repair of liver tissues following injury. However, whether TSA improves the function of liver cells and protects the liver from injury by enhancing the growth and proliferation of hepatic oval cells remains to be elucidated. In the present study, low to moderate concentrations of TSA were observed to stimulate proliferation, did not induce apoptosis in WB‑F344 rat hepatic oval cells and the increased expression levels of β‑catenin. WB‑F344 cells were treated with various concentrations of TSA (0‑80 µg/ml) for 24, 48, 72 and 96 h. Cell proliferation was measured using a Cell Counting kit‑8 (CCK‑8) assay, a 5‑ethynyl‑2'‑deoxyuridine assay and a carboxyfluorescein diacetate succinimidyl ester (CFSE) assay. The CCK‑8 assay demonstrated that treatment of WB‑F344 cells with 20‑40 µg/ml TSA for up to 72 h significantly increased proliferation. Similar results were observed in the subsequent EdU and CFSE assays. Furthermore, a terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay demonstrated that 20‑40 µg/ml TSA treatment for up to 96 h did not induce apoptosis of the WB‑F344 cells. Notably, the results of western blot, immunofluorescence and reverse transcription‑quantitative polymerase chain reaction analyses demonstrated that treatment of the WB‑F344 cells with 20‑40 µg/ml TSA for up to 72 h significantly increased the expression levels of β‑catenin. These data indicated that TSA at concentrations between 20 and 40 µg/ml may induce WB‑F344 cell proliferation by activating the canonical Wnt signaling pathway. The results of the present study suggest that TSA may be a useful natural agent to enhance repair and regeneration of the injured liver, and improve liver regeneration following orthotopic liver transplantation.
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1 

Zhang Y, Li XM, Zhang FK and Wang BE: Activation of canonical Wnt signaling pathway promotes proliferation and self-renewal of rat hepatic oval cell line WB-F344 in vitro. World J Gastroenterol. 14:6673–6680. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Lowes KN, Croager EJ, Olynyk JK, Abraham LJ and Yeoh GC: Oval cell-mediated liver regeneration: Role of cytokines and growth factors. J Gastroenterol Hepatol. 18:4–12. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Walkup MH and Gerber DA: Hepatic stem cells: In search of. Stem Cells. 24:1833–1840. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Farber E: Similarities in the sequence of early histological changes induced in the liver of the rat by ethionine, 2-acety-lamino-fluorene, and 3′-methyl-4-dimethylaminoazobenzene. Cancer Res. 16:142–148. 1956.PubMed/NCBI

5 

Thorgeirsson SS: Hepatic stem cells. Am J Pathol. 142:1331–1333. 1993.PubMed/NCBI

6 

Fausto N: Oval cells and liver carcinogenesis: An analysis of cell lineages in hepatic tumors using oncogene transfection techniques. Prog Clin Biol Res. 331:325–334. 1990.PubMed/NCBI

7 

Sell S: Is there a liver stem cell? Cancer Res. 50:3811–3815. 1990.PubMed/NCBI

8 

Sigal SH, Brill S, Fiorino AS and Reid LM: The liver as a stem cell and lineage system. Am J Physiol. 263:G139–G148. 1992.PubMed/NCBI

9 

Pan TL and Wang PW: Explore the molecular mechanism of apoptosis induced by tanshinone IIA on activated rat hepatic stellate cells. Evid Based Complement Alternat Med. 2012:7349872012. View Article : Google Scholar

10 

Che XH, Park EJ, Zhao YZ, Kim WH and Sohn DH: Tanshinone II A induces apoptosis and S phase cell cycle arrest in activated rat hepatic stellate cells. Basic Clin Pharmacol Toxicol. 106:30–37. 2010.

11 

Niu XH, Hua HY, Guo WJ, Zhang Y, Liu M, Hong Y, Wu PF, Lu P and Zhang HF: Clinical efficiency of tanshinone IIA-sulfonate in treatment of liver fibrosis of advanced schistosomiasis. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 25:137–140. 2013.In Chinese. PubMed/NCBI

12 

Sun RF, Liu LX and Zhang HY: Effect of tanshinone II on hepatic fibrosis in mice. Zhongguo Zhong Xi Yi Jie He Za Zhi. 29:1012–1017. 2009.In Chinese.

13 

Clevers H, Loh KM and Nusse R: Stem cell signaling. An integral program for tissue renewal and regeneration: Wnt signaling and stem cell control. Science. 346:12480122014. View Article : Google Scholar : PubMed/NCBI

14 

Willert K, Brown JD, Danenberg E, Duncan AW, Weissman IL, Reya T, Yates JR III and Nusse R: Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature. 423:448–452. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Reis M and Liebner S: Wnt signaling in the vasculature. Exp Cell Res. 319:1317–1323. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Micsenyi A, Tan X, Sneddon T, Luo JH, Michalopoulos GK and Monga SP: Beta-catenin is temporally regulated during normal liver development. Gastroenterology. 126:1134–1146. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Pan L, Shi X, Liu S, Guo X, Zhao M, Cai R and Sun G: Fluoride promotes osteoblastic differentiation through canonical Wnt/β-catenin signaling pathway. Toxicol Lett. 225:34–42. 2014. View Article : Google Scholar

18 

Saraste A, Arola A, Vuorinen T, Kytö V, Kallajoki M, Pulkki K, Voipio-Pulkki LM and Hyypiä T: Cardiomyocyte apoptosis in experimental coxsackievirus B3 myocarditis. Cardiovasc Pathol. 12:255–262. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Li Z, Chen J, Li L, Ran JH, Liu J, Gao TX, Guo BY, Li XH, Liu ZH, Liu GJ, et al: In vitro proliferation and differentiation of hepatic oval cells and their potential capacity for intrahepatic transplantation. Braz J Med Biol Res. 46:681–688. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Dezső K, Papp V, Bugyik E, Hegyesi H, Sáfrány G, Bödör C, Nagy P and Paku S: Structural analysis of oval-cell-mediated liver regeneration in rats. Hepatology. 56:1457–1467. 2012. View Article : Google Scholar

21 

Li WQ, Li YM, Guo J, Liu YM, Yang XQ, Ge HJ, Xu Y, Liu HM, He J and Yu HY: Hepatocytic precursor (stem-like) WB-F344 cells reduce tumorigenicity of hepatoma CBRH-7919 cells via TGF-beta/Smad pathway. Oncol Rep. 23:1601–1607. 2010.PubMed/NCBI

22 

Wang P, Cong M, Liu TH, Yang AT, Cong R, Wu P, Tang SZ, Xu Y, Wang H, Wang BE, et al: Primary isolated hepatic oval cells maintain progenitor cell phenotypes after two-year prolonged cultivation. J Hepatol. 53:863–871. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Lin YL, Hsu YC, Chiu YT and Huang YT: Antifibrotic effects of a herbal combination regimen on hepatic fibrotic rats. Phytother Res. 22:69–76. 2008. View Article : Google Scholar

24 

Zhang H, Long M, Wu Z, Han X and Yu Y: Sodium tanshinone IIA silate as an add-on therapy in patients with unstable angina pectoris. J Thorac Dis. 6:1794–1799. 2014.

25 

Mao S, Wang L, Zhao X, Shang H, Zhang M and Hinek A: Sodium tanshinone IIA sulfonate for reduction of periprocedural myocardial injury during percutaneous coronary intervention (STAMP trial): Rationale and design. Int J Cardiol. 182:329–333. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Pang H, Han B, Yu T and Peng Z: The complex regulation of tanshinone IIA in rats with hypertension-induced left ventricular hypertrophy. PLoS One. 9:e922162014. View Article : Google Scholar : PubMed/NCBI

27 

Mao S, Wang Y, Zhang M and Hinek A: Phytoestrogen, tanshinone IIA diminishes collagen deposition and stimulates new elastogenesis in cultures of human cardiac fibroblasts. Exp Cell Res. 323:189–197. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Yu Q, Chen H, Sheng L, Liang Y and Li Q: Sodium tanshinone IIA sulfonate prolongs the survival of skin allografts by inhibiting inflammatory cell infiltration and T cell proliferation. Int Immunopharmacol. 22:277–284. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Yang L, Guo H, Dong L, Wang L, Liu C and Wang X: Tanshinone IIA inhibits the growth, attenuates the stemness and induces the apoptosis of human glioma stem cells. Oncol Rep. 32:1303–1311. 2014.PubMed/NCBI

30 

Shan QQ, Guo Y and Gong YP: Effect of tanshinone II A on leukemia cell line K562. Sichuan Da Xue Xue Bao Yi Xue Ban. 45:410–413. 2014.PubMed/NCBI

31 

Schotanus BA, Kruitwagen HS, van den Ingh TS, van Wolferen ME, Rothuizen J, Penning LC and Spee B: Enhanced Wnt/β-catenin and Notch signalling in the activated canine hepatic progenitor cell niche. BMC Vet Res. 10:3092014. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Ze X, Jia J, Li X, You H, Zhao X, Zhang D and Wang B: Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling. Mol Med Rep 13: 1501-1508, 2016.
APA
Ze, X., Jia, J., Li, X., You, H., Zhao, X., Zhang, D., & Wang, B. (2016). Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling. Molecular Medicine Reports, 13, 1501-1508. https://doi.org/10.3892/mmr.2015.4696
MLA
Ze, X., Jia, J., Li, X., You, H., Zhao, X., Zhang, D., Wang, B."Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling". Molecular Medicine Reports 13.2 (2016): 1501-1508.
Chicago
Ze, X., Jia, J., Li, X., You, H., Zhao, X., Zhang, D., Wang, B."Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling". Molecular Medicine Reports 13, no. 2 (2016): 1501-1508. https://doi.org/10.3892/mmr.2015.4696
Copy and paste a formatted citation
x
Spandidos Publications style
Ze X, Jia J, Li X, You H, Zhao X, Zhang D and Wang B: Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling. Mol Med Rep 13: 1501-1508, 2016.
APA
Ze, X., Jia, J., Li, X., You, H., Zhao, X., Zhang, D., & Wang, B. (2016). Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling. Molecular Medicine Reports, 13, 1501-1508. https://doi.org/10.3892/mmr.2015.4696
MLA
Ze, X., Jia, J., Li, X., You, H., Zhao, X., Zhang, D., Wang, B."Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling". Molecular Medicine Reports 13.2 (2016): 1501-1508.
Chicago
Ze, X., Jia, J., Li, X., You, H., Zhao, X., Zhang, D., Wang, B."Tanshinone IIA promotes the proliferation of WB‑F344 hepatic oval cells via Wnt/β‑catenin signaling". Molecular Medicine Reports 13, no. 2 (2016): 1501-1508. https://doi.org/10.3892/mmr.2015.4696
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