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Article Open Access

Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells

  • Authors:
    • Gui‑Xing Jiang
    • Li‑Ping Cao
    • Peng‑Cheng Kang
    • Xiang‑Yu Zhong
    • Tian‑Yu Lin
    • Yun‑Fu Cui
  • View Affiliations / Copyright

    Affiliations: Department of Hepatopancreatobiliary Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China, Department of Hepatopancreatobiliary Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1563-1569
    |
    Published online on: December 22, 2015
       https://doi.org/10.3892/mmr.2015.4706
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Abstract

The aim of the present study was to determine the role of interleukin‑6 (IL‑6) in the epithelial‑mesenchymal transition (EMT) of human intrahepatic biliary epithelial cell (HIBEC) lines in vitro. HIBECs were stimulated with IL‑6 at concentrations of 0, 10, 20, 50 and 100 µg/l for 24 h. A wound healing and Transwell assay were performed to determine the migratory and invasive capacity of HIBECs, respectively. Following 24 h of incubation, IL-6 at 10 and 20 µg/l significantly increased the number of migrated and invaded cells (P<0.05), while stimulation with 50 and 100 µg/l IL‑6 resulted in a further increase of the migratory and invasive capacity compared to that in all other groups (P<0.05). Furthermore, reverse-transcription quantitative polymerase chain reaction and western blot analyses were used to detect the mRNA and protein expression of EMT markers E‑cadherin and vimentin in HIBECs. Decreased mRNA levels of E‑cadherin accompanied by higher mRNA levels of vimentin were observed in the 10, 20, 50, 100 µg/l IL-6 groups compared to those in the 0 µg/l group (all P<0.05). Furthermore, the protein expression of E‑cadherin was decreased, while that of vimentin was increased in the 50 and 100 µg/l IL-6 groups compared to those in the 0, 10 and 20 µg/l IL-6 groups (all P<0.05). The present study therefore indicated that IL‑6 promoted the process of EMT in HIBECs as characterized by increased migration and invasion of HIBECs and the typical changes in mRNA and protein expression of the EMT markers E‑cadherin and vimentin.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang GX, Cao LP, Kang PC, Zhong XY, Lin TY and Cui YF: Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells. Mol Med Rep 13: 1563-1569, 2016.
APA
Jiang, G., Cao, L., Kang, P., Zhong, X., Lin, T., & Cui, Y. (2016). Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells. Molecular Medicine Reports, 13, 1563-1569. https://doi.org/10.3892/mmr.2015.4706
MLA
Jiang, G., Cao, L., Kang, P., Zhong, X., Lin, T., Cui, Y."Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells". Molecular Medicine Reports 13.2 (2016): 1563-1569.
Chicago
Jiang, G., Cao, L., Kang, P., Zhong, X., Lin, T., Cui, Y."Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells". Molecular Medicine Reports 13, no. 2 (2016): 1563-1569. https://doi.org/10.3892/mmr.2015.4706
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang GX, Cao LP, Kang PC, Zhong XY, Lin TY and Cui YF: Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells. Mol Med Rep 13: 1563-1569, 2016.
APA
Jiang, G., Cao, L., Kang, P., Zhong, X., Lin, T., & Cui, Y. (2016). Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells. Molecular Medicine Reports, 13, 1563-1569. https://doi.org/10.3892/mmr.2015.4706
MLA
Jiang, G., Cao, L., Kang, P., Zhong, X., Lin, T., Cui, Y."Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells". Molecular Medicine Reports 13.2 (2016): 1563-1569.
Chicago
Jiang, G., Cao, L., Kang, P., Zhong, X., Lin, T., Cui, Y."Interleukin‑6 induces epithelial‑mesenchymal transition in human intrahepatic biliary epithelial cells". Molecular Medicine Reports 13, no. 2 (2016): 1563-1569. https://doi.org/10.3892/mmr.2015.4706
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