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Article Open Access

Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice

  • Authors:
    • Ying Lin
    • Jianhui Zhuang
    • Hailing Li
    • Guofu Zhu
    • Shunping Zhou
    • Weiming Li
    • Wenhui Peng
    • Yawei Xu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai 200072, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1509-1516
    |
    Published online on: December 22, 2015
       https://doi.org/10.3892/mmr.2015.4708
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Abstract

Visceral adipose tissue‑derived serine protease inhibitor (vaspin) is a novel adipokine with potential insulin‑sensitizing properties, which was initially detected in the visceral adipose tissue of genetically obese rats. Previous studies have demonstrated that vaspin exerts a protective effect on arteries undergoing atherosclerosis in vitro, and it has been shown to exert anti‑inflammatory and antimigratory effects on vascular smooth muscle cells. Vaspin promotes proliferation and inhibits apoptosis in endothelial cells, and decreases proliferation of the arterial intima under diabetic conditions. In addition, macrophage apoptosis is an important characteristic of atherosclerotic plaque development. In vivo experiments were performed by histological analysis, including Oil Red O, hematoxylin and eosin and Masson's trichrome staining. Mice were injected with lentivirus via the tail vein and tissues were obtained for histological analysis. Cell apoptosis was determined by flow cytometry of Annexin‑V/propidium iodide dual staining and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling assay. Total proteins were extracted and protein expression levels were detected by western blot analysis. The present study aimed to investigate whether vaspin was able to protect against atherosclerotic development in vivo, and to explore the underlying mechanisms of the potential antiatherogenic effects. The results of the current study indicated that vaspin inhibited the progression of atherosclerotic plaques in apoE‑/‑ mice by inhibiting endoplasmic reticulum stress‑induced macrophage apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Lin Y, Zhuang J, Li H, Zhu G, Zhou S, Li W, Peng W and Xu Y: Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice. Mol Med Rep 13: 1509-1516, 2016.
APA
Lin, Y., Zhuang, J., Li, H., Zhu, G., Zhou, S., Li, W. ... Xu, Y. (2016). Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice. Molecular Medicine Reports, 13, 1509-1516. https://doi.org/10.3892/mmr.2015.4708
MLA
Lin, Y., Zhuang, J., Li, H., Zhu, G., Zhou, S., Li, W., Peng, W., Xu, Y."Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice". Molecular Medicine Reports 13.2 (2016): 1509-1516.
Chicago
Lin, Y., Zhuang, J., Li, H., Zhu, G., Zhou, S., Li, W., Peng, W., Xu, Y."Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice". Molecular Medicine Reports 13, no. 2 (2016): 1509-1516. https://doi.org/10.3892/mmr.2015.4708
Copy and paste a formatted citation
x
Spandidos Publications style
Lin Y, Zhuang J, Li H, Zhu G, Zhou S, Li W, Peng W and Xu Y: Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice. Mol Med Rep 13: 1509-1516, 2016.
APA
Lin, Y., Zhuang, J., Li, H., Zhu, G., Zhou, S., Li, W. ... Xu, Y. (2016). Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice. Molecular Medicine Reports, 13, 1509-1516. https://doi.org/10.3892/mmr.2015.4708
MLA
Lin, Y., Zhuang, J., Li, H., Zhu, G., Zhou, S., Li, W., Peng, W., Xu, Y."Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice". Molecular Medicine Reports 13.2 (2016): 1509-1516.
Chicago
Lin, Y., Zhuang, J., Li, H., Zhu, G., Zhou, S., Li, W., Peng, W., Xu, Y."Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress‑induced macrophage apoptosis in apoE‑/‑ mice". Molecular Medicine Reports 13, no. 2 (2016): 1509-1516. https://doi.org/10.3892/mmr.2015.4708
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