Open Access

Selective regulation of neurosteroid biosynthesis under ketamine-induced apoptosis of cortical neurons in vitro

  • Authors:
    • Jianli Li
    • Yang Yu
    • Bei Wang
    • Honghai Wu
    • Gai Xue
    • Yanning Hou
  • View Affiliations

  • Published online on: December 23, 2015     https://doi.org/10.3892/mmr.2015.4712
  • Pages: 1586-1592
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Numerous studies have suggested that ketamine administration can induce neuroapoptosis in primary cultured cortical neurons. Neurosteroids modulate neuronal function and serve important roles in the central nervous system, however the role of neurosteroids in neuroapoptosis induced by ketamine remains to be elucidated. The present study aimed to explore whether neurosteroidogenesis was a pivotal mechanism for neuroprotection against ketamine-induced neuroapoptosis, and whether it may be selectively regulated under ketamine-induced neuroapoptosis conditions in primary cultured cortical neurons. To study this hypothesis, the effect of ketamine exposure on neurosteroidogenesis in primary cultured cortical neurons was investigated. Cholesterol, a substrate involved in the synthesis of neurosteroids, was added to the culture medium, and neurosteroids were quantified using high‑performance liquid chromatography‑tandem mass spectrometry analysis. The data demonstrated that cholesterol blocked ketamine‑induced neuroapoptosis by promoting the synthesis of various neurosteroids, and the pathway of neurosteroid testosterone conversion into estradiol was inhibited by ketamine exposure. These data suggest that endogenous neurosteroids biosynthesis is critical for neuroprotection against ketamine-induced neuroapoptosis and inhibiting the biosynthesis of neuroprotective-neurosteroid estradiol is of notable importance for ketamine-induced neuroapoptosis.
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February-2016
Volume 13 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li J, Yu Y, Wang B, Wu H, Xue G and Hou Y: Selective regulation of neurosteroid biosynthesis under ketamine-induced apoptosis of cortical neurons in vitro. Mol Med Rep 13: 1586-1592, 2016
APA
Li, J., Yu, Y., Wang, B., Wu, H., Xue, G., & Hou, Y. (2016). Selective regulation of neurosteroid biosynthesis under ketamine-induced apoptosis of cortical neurons in vitro. Molecular Medicine Reports, 13, 1586-1592. https://doi.org/10.3892/mmr.2015.4712
MLA
Li, J., Yu, Y., Wang, B., Wu, H., Xue, G., Hou, Y."Selective regulation of neurosteroid biosynthesis under ketamine-induced apoptosis of cortical neurons in vitro". Molecular Medicine Reports 13.2 (2016): 1586-1592.
Chicago
Li, J., Yu, Y., Wang, B., Wu, H., Xue, G., Hou, Y."Selective regulation of neurosteroid biosynthesis under ketamine-induced apoptosis of cortical neurons in vitro". Molecular Medicine Reports 13, no. 2 (2016): 1586-1592. https://doi.org/10.3892/mmr.2015.4712