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Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway

  • Authors:
    • Hua Zhang
    • Zhouyi Xiong
    • Jiao Wang
    • Shuangshuang Zhang
    • Lei Lei
    • Li Yang
    • Zhen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510282, P.R. China, Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1593-1601
    |
    Published online on: December 28, 2015
       https://doi.org/10.3892/mmr.2015.4724
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Abstract

Cardiomyocyte apoptosis is a major event in the pathogenesis of diabetic cardiomyopathy. Currently, no single effective treatment for diabetic cardiomyopathy exists. The present study investigated whether advanced oxidative protein products (AOPPs) have a detrimental role in the survival of cardiomyocytes and if glucagon‑like peptide‑1 (GLP‑1) exerts a cardioprotective effect under these circumstances. The present study also aimed to determine the underlying mechanisms. H9c2 cells were exposed to increasing concentrations of AOPPs in the presence or absence of GLP‑1, and the viability and apoptotic rate were detected using a cell counting kit‑8 assay and flow cytometry, respectively. In addition, a phosphatidylinositol‑4,5‑bisphosphate 3‑kinase (PI3K) inhibitor, LY294002, was employed to illustrate the mechanism of the antiapoptotic effect of GLP‑1. The expression levels of the apoptotic‑associated proteins, Akt, B‑cell lymphoma (Bcl)‑2, Bcl‑2‑associated death promoter (Bad), Bcl‑2‑associated X protein (Bax) and caspase‑3 were measured by western blotting. It was revealed that GLP‑1 significantly attenuated AOPP‑induced cell toxicity and apoptosis. AOPPs inactivated the phosphorylation of Akt, reduced the phosphorylation of Bad, decreased the expression of Bcl‑2, increased the expression of Bax and the activation of caspase‑3 in H9c2 cells. GLP‑1 reversed the above changes induced by AOPPs and the protective effects of GLP‑1 were abolished by the PI3K inhibitor, LY294002. In conclusion, the present data suggested that GLP‑1 protected cardiomyocytes against AOPP‑induced apoptosis, predominantly via the PI3K/Akt/Bad pathway. These results provided a conceivable mechanism for the development of diabetic cardiomyopathy and rendered a novel application of GLP‑1 exerting favorable cardiac effects for the treatment of diabetic cardiomyopathy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Xiong Z, Wang J, Zhang S, Lei L, Yang L and Zhang Z: Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway. Mol Med Rep 13: 1593-1601, 2016.
APA
Zhang, H., Xiong, Z., Wang, J., Zhang, S., Lei, L., Yang, L., & Zhang, Z. (2016). Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway. Molecular Medicine Reports, 13, 1593-1601. https://doi.org/10.3892/mmr.2015.4724
MLA
Zhang, H., Xiong, Z., Wang, J., Zhang, S., Lei, L., Yang, L., Zhang, Z."Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway". Molecular Medicine Reports 13.2 (2016): 1593-1601.
Chicago
Zhang, H., Xiong, Z., Wang, J., Zhang, S., Lei, L., Yang, L., Zhang, Z."Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway". Molecular Medicine Reports 13, no. 2 (2016): 1593-1601. https://doi.org/10.3892/mmr.2015.4724
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Xiong Z, Wang J, Zhang S, Lei L, Yang L and Zhang Z: Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway. Mol Med Rep 13: 1593-1601, 2016.
APA
Zhang, H., Xiong, Z., Wang, J., Zhang, S., Lei, L., Yang, L., & Zhang, Z. (2016). Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway. Molecular Medicine Reports, 13, 1593-1601. https://doi.org/10.3892/mmr.2015.4724
MLA
Zhang, H., Xiong, Z., Wang, J., Zhang, S., Lei, L., Yang, L., Zhang, Z."Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway". Molecular Medicine Reports 13.2 (2016): 1593-1601.
Chicago
Zhang, H., Xiong, Z., Wang, J., Zhang, S., Lei, L., Yang, L., Zhang, Z."Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway". Molecular Medicine Reports 13, no. 2 (2016): 1593-1601. https://doi.org/10.3892/mmr.2015.4724
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