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Article

Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma

  • Authors:
    • Fabrício P. Nascimento
    • Mirian G. Cardoso
    • Susan C. Lindsey
    • Ilda S. Kunii
    • Flávia O.F. Valente
    • Marina M.L. Kizys
    • Rosana Delcelo
    • Cléber P. Camacho
    • Rui M.B. Maciel
    • Magnus R. Dias‑Da‑Silva
  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular and Translational Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, Sao Paulo 04039‑032, Brazil, Department of Pathology, Escola Paulista de Medicina, Universidade Federal de São Paulo, Sao Paulo 04039‑032, Brazil
  • Pages: 1653-1660
    |
    Published online on: December 29, 2015
       https://doi.org/10.3892/mmr.2015.4731
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Abstract

Medullary thyroid carcinoma (MTC), a neuroendocrine tumor originating from thyroid parafollicular cells, has been demonstrated to be associated with mutations in RET, HRAS, KRAS and NRAS. However, the role of other genes involved in the oncogenesis of neural crest tumors remains to be fully investigated in MTC. The current study aimed to investigate the presence of somatic mutations in BRAF, CDKN2A and PI3KCA in MTC, and to investigate the correlation with disease progression. DNA was isolated from paraffin‑embedded tumors and blood samples from patients with MTC, and the hotspot somatic mutations were sequenced. A total of 2 novel HRAS mutations, p.Asp33Asn and p.His94Tyr, and polymorphisms within the 3' untranslated region (UTR) of CDKN2A (rs11515 and rs3088440) were identified, however, no mutations were observed in other genes. It was suggested that somatic point mutations in BRAF, CDKN2A and PI3KCA do not participate in the oncogenesis of MTC. Further studies are required in order to clarify the contribution of the polymorphisms identified in the 3'UTR of CDKN2A in MTC.
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View References

1 

American Thyroid Association Guidelines Task Force; Kloos RT, Eng C, Evans DB, Francis GL, Gagel RF, Gharib H, Moley JF, Pacini F and Ringel MD: Medullary thyroid cancer: Management guidelines of the American thyroid association. Thyroid. 19:565–612. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Nosé V: Familial thyroid cancer: A review. Mod Pathol. 24(Suppl 2): S19–S33. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Moura MM, Cavaco BM, Pinto AE and Leite V: High prevalence of RAS mutations in RET-negative sporadic medullary thyroid carcinomas. J Clin Endocrinol Metab. 96:E863–E868. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Ciampi R, Mian C, Fugazzola L, Cosci B, Romei C, Barollo S, Cirello V, Bottici V, Marconcini G, Rosa PM, et al: Evidence of a low prevalence of RAS mutations in a large medullary thyroid cancer series. Thyroid. 23:50–57. 2013. View Article : Google Scholar

5 

Agrawal N, Jiao Y, Sausen M, Leary R, Bettegowda C, Roberts NJ, Bhan S, Ho AS, Khan Z, Bishop J, et al: Exomic sequencing of medullary thyroid cancer reveals dominant and mutually exclusive oncogenic mutations in RET and RAS. J Clin Endocrinol Metab. 98:E364–E369. 2013. View Article : Google Scholar :

6 

Tamburrino A, Molinolo AA, Salerno P, Chernock RD, Raffeld M, Xi L, Gutkind JS, Moley JF, Wells SA Jr and Santoro M: Activation of the mTOR pathway in primary medullary thyroid carcinoma and lymph node metastases. Clin Cancer Res. 18:3532–3540. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Simbolo M, Mian C, Barollo S, Fassan M, Mafficini A, Neves D, Scardoni M, Pennelli G, Rugge M, Pelizzo MR, et al: High-throughput mutation profiling improves diagnostic stratification of sporadic medullary thyroid carcinomas. Virchows Arch. 465:73–78. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Puppin C, Durante C, Sponziello M, Verrienti A, Pecce V, Lavarone E, Baldan F, Campese AF, Boichard A, Lacroix L, et al: Overexpression of genes involved in miRNA biogenesis in medullary thyroid carcinomas with RET mutation. Endocrine. 47:528–536. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Rapa I, Saggiorato E, Giachino D, Palestini N, Orlandi F, Papotti M and Volante M: Mammalian target of rapamycin pathway activation is associated to RET mutation status in medullary thyroid carcinoma. J Clin Endocrinol Metab. 96:2146–2153. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Berrocal A, Cabañas L, Espinosa E, Fernández-de-Misa R, Martín-Algarra S, Martínez-Cedres JC, Ríos-Buceta L and Rodríguez-Peralto JL: Melanoma: Diagnosis, staging and treatment. Consensus group recommendations. Adv Ther. 31:945–960. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Muscarella P, Bloomston M, Brewer AR, Mahajan A, Frankel WL, Ellison EC, Farrar WB, Weghorst CM and Li J: Expression of the p16INK4A/Cdkn2a gene is prevalently downregulated in human pheochromocytoma tumor specimens. Gene Expr. 14:207–216. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Güran S and Tali ET: p53 and p16INK4A mutations during the progression of glomus tumor. Pathol Oncol Res. 5:41–45. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Nikiforova MN, Kimura ET, Gandhi M, Biddinger PW, Knauf JA, Basolo F, Zhu Z, Giannini R, Salvatore G, Fusco A, et al: BRAF mutations in thyroid tumors are restricted to papillary carcinomas and anaplastic or poorly differentiated carcinomas arising from papillary carcinomas. J Clin Endocrinol Metab. 88:5399–5404. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Goutas N, Vlachodimitropoulos D, Bouka M, Lazaris AC, Nasioulas G and Gazouli M: BRAF and K-RAS mutation in a Greek papillary and medullary thyroid carcinoma cohort. Anticancer Res. 28:305–308. 2008.PubMed/NCBI

15 

Schulten HJ, Al-Maghrabi J, Al-Ghamdi K, Salama S, Al-Muhayawi S, Chaudhary A, Hamour O, Abuzenadah A, Gari M and Al-Qahtani M: Mutational screening of RET, HRAS, KRAS, NRAS, BRAF, AKT1 and CTNNB1 in medullary thyroid carcinoma. Anticancer Res. 31:4179–4183. 2011.PubMed/NCBI

16 

Boichard A, Croux L, Al Ghuzlan A, Broutin S, Dupuy C, Leboulleux S, Schlumberger M, Bidart JM and Lacroix L: Somatic RAS mutations occur in a large proportion of sporadic RET-negative medullary thyroid carcinomas and extend to a previously unidentified exon. J Clin Endocrinol Metab. 97:E2031–E2035. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Sauroja I, Smeds J, Vlaykova T, Kumar R, Talve L, Hahka-Kemppinen M, Punnonen K, Jansèn CT, Hemminki K and Pyrhönen S: Analysis of G(1)/S checkpoint regulators in metastatic melanoma. Genes Chromosomes Cancer. 28:404–414. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Pasquali D, Circelli L, Faggiano A, Pancione M, Renzullo A, Elisei R, Romei C, Accardo G, Coppola VR, De Palma M, et al: CDKN1B V109G polymorphism a new prognostic factor in sporadic medullary thyroid carcinoma. Eur J Endocrinol. 164:397–404. 2011. View Article : Google Scholar

19 

Tian Q, Frierson HF Jr, Krystal GW and Moskaluk CA: Activating c-kit gene mutations in human germ cell tumors. Am J Pathol. 154:1643–1647. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Samuels Y and Ericson K: Oncogenic PI3K and its role in cancer. Curr Opin Oncol. 18:77–82. 2006. View Article : Google Scholar

21 

Kizys MM, Cardoso MG, Lindsey SC, Harada MY, Soares FA, Melo MC, Montoya MZ, Kasamatsu TS, Kunii IS, Giannocco G, et al: Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues and long-term stored blood samples. Arq Bras Endocrinol Metabol. 56:618–626. 2012. View Article : Google Scholar

22 

Venselaar H, Te Beek TA, Kuipers RK, Hekkelman ML and Vriend G: Protein structure analysis of mutations causing inheritable diseases. An e-Science approach with life scientist friendly interfaces. BMC Bioinformatics. 11:5482010. View Article : Google Scholar : PubMed/NCBI

23 

Lee PH and Shatkay H: F-SNP: Computationally predicted functional SNPs for disease association studies. Nucleic Acids Res. 36(Database issue): D820–D824. 2008. View Article : Google Scholar :

24 

Edge SE, Byrd DR, Carducci MA, Compton CC, Fritz AG, Greene F and Trotti A: AJCC Cancer Staging Manual. Springer-Verlag; New York: 2009

25 

Lindsey SC, Kunii IS, Germano-Neto F, Sittoni MY, Camacho CP, Valente FO, Yang JH, Signorini PS, Delcelo R, Cerutti JM, et al: Extended RET gene analysis in patients with apparently sporadic medullary thyroid cancer: Clinical benefits and cost. Horm Cancer. 3:181–186. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Kumar R, Smeds J, Berggren P, Straume O, Rozell BL, Akslen LA and Hemminki K: A single nucleotide polymorphism in the 3′untranslated region of the CDKN2A gene is common in sporadic primary melanomas but mutations in the CDKN2B, CDKN2C, CDK4 and p53 genes are rare. Int J Cancer. 95:388–393. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Chatterjee S and Pal JK: Role of 5′- and 3′-untranslated regions of mRNAs in human diseases. Biol Cell. 101:251–262. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Sakano S, Berggren P, Kumar R, Steineck G, Adolfsson J, Onelöv E, Hemminki K and Larsson P: Clinical course of bladder neoplasms and single nucleotide polymorphisms in the CDKN2A gene. Int J Cancer. 104:98–103. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Geddert H, Kiel S, Zotz RB, Zhang J, Willers R, Gabbert HE and Sarbia M: Polymorphism of p16 INK4A and cyclin D1 in adenocarcinomas of the upper gastrointestinal tract. J Cancer Res Clin Oncol. 131:803–808. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Chansaenroj J, Theamboonlers A, Junyangdikul P, Swangvaree S, Karalak A, Chinchai T and Poovorawan Y: Polymorphisms in TP53 (rs1042522), p16 (rs11515 and rs3088440) and NQO1 (rs1800566) genes in Thai cervical cancer patients with HPV 16 infection. Asian Pac J Cancer Prev. 14:341–346. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Straume O, Smeds J, Kumar R, Hemminki K and Akslen LA: Significant impact of promoter hypermethylation and the 540 C>T polymorphism of CDKN2A in cutaneous melanoma of the vertical growth phase. Am J Pathol. 161:229–237. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Boonstra JJ, van Marion R, Tilanus HW and Dinjens WN: Functional polymorphisms associated with disease-free survival in resected carcinoma of the esophagus. J Gastrointest Surg. 15:48–56. 2011. View Article : Google Scholar :

33 

Pinheiro UB, de Carvalho Fraga CA, Mendes DC, Marques-Silva L, Farias LC, de Souza MG, Soares MB, Jones KM, Santos SH, de Paula AM, et al: p16 (CDKN2A) SNP rs11515 was not associated with head and neck carcinoma. Tumour Biol. 35:6113–6118. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Jin L, Xu L, Song X, Wei Q, Sturgis EM and Li G: Genetic variation in MDM2 and p14ARF and susceptibility to salivary gland carcinoma. PloS One. 7:e493612012. View Article : Google Scholar : PubMed/NCBI

35 

Polakova V, Pardini B, Naccarati A, Landi S, Slyskova J, Novotny J, Vodickova L, Bermejo JL, Hanova M, Smerhovsky Z, et al: Genotype and haplotype analysis of cell cycle genes in sporadic colorectal cancer in the czech republic. Hum Mutat. 30:661–668. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Royds JA, Al Nadaf S, Wiles AK, Chen YJ, Ahn A, Shaw A, Bowie S, Lam F, Baguley BC, Braithwaite AW, et al: The CDKN2A G500 allele is more frequent in GBM patients with no defined telomere maintenance mechanism tumors and is associated with poorer survival. PloS One. 6:e267372011. View Article : Google Scholar : PubMed/NCBI

37 

Thakur N, Hussain S, Nasare V, Das BC, Basir SF and Bharadwaj M: Association analysis of p16 (CDKN2A) and RB1 polymorphisms with susceptibility to cervical cancer in Indian population. Mol Biol Rep. 39:407–414. 2012. View Article : Google Scholar

38 

Zhang Y, Sturgis EM, Zafereo ME, Wei Q and Li G: p14ARF genetic polymorphisms and susceptibility to second primary malignancy in patients with index squamous cell carcinoma of the head and neck. Cancer. 117:1227–1235. 2011. View Article : Google Scholar : PubMed/NCBI

39 

Zhang F, Xu L, Wei Q, Song X, Sturgis EM and Li G: Significance of MDM2 and P14 ARF polymorphisms in susceptibility to differentiated thyroid carcinoma. Surgery. 153:711–717. 2013. View Article : Google Scholar :

40 

De Giorgi V, Savarese I, D'Errico A, Gori A, Papi F, Colombino M, Cristina Sini M, Stanganelli I, Palmieri G and Massi D: CDKN2A mutations could influence the dermoscopic pattern of presentation of multiple primary melanoma: A clinical dermoscopic genetic study. J Eur Acad Dermatol Venereol. 29:574–580. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Song X, Sturgis EM, Huang Z, Li X, Li C, Wei Q and Li G: Potentially functional variants of p14ARF are associated with HPV-positive oropharyngeal cancer patients and survival after definitive chemoradiotherapy. Carcinogenesis. 35:62–68. 2014. View Article : Google Scholar

42 

Stenman G, Andersson MK and Andrén Y: New tricks from an old oncogene: Gene fusion and copy number alterations of MYB in human cancer. Cell Cycle. 9:2986–2995. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Nascimento FP, Cardoso MG, Lindsey SC, Kunii IS, Valente FO, Kizys MM, Delcelo R, Camacho CP, Maciel RM, Dias‑Da‑Silva MR, Dias‑Da‑Silva MR, et al: Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma. Mol Med Rep 13: 1653-1660, 2016.
APA
Nascimento, F.P., Cardoso, M.G., Lindsey, S.C., Kunii, I.S., Valente, F.O., Kizys, M.M. ... Dias‑Da‑Silva, M.R. (2016). Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma. Molecular Medicine Reports, 13, 1653-1660. https://doi.org/10.3892/mmr.2015.4731
MLA
Nascimento, F. P., Cardoso, M. G., Lindsey, S. C., Kunii, I. S., Valente, F. O., Kizys, M. M., Delcelo, R., Camacho, C. P., Maciel, R. M., Dias‑Da‑Silva, M. R."Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma". Molecular Medicine Reports 13.2 (2016): 1653-1660.
Chicago
Nascimento, F. P., Cardoso, M. G., Lindsey, S. C., Kunii, I. S., Valente, F. O., Kizys, M. M., Delcelo, R., Camacho, C. P., Maciel, R. M., Dias‑Da‑Silva, M. R."Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma". Molecular Medicine Reports 13, no. 2 (2016): 1653-1660. https://doi.org/10.3892/mmr.2015.4731
Copy and paste a formatted citation
x
Spandidos Publications style
Nascimento FP, Cardoso MG, Lindsey SC, Kunii IS, Valente FO, Kizys MM, Delcelo R, Camacho CP, Maciel RM, Dias‑Da‑Silva MR, Dias‑Da‑Silva MR, et al: Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma. Mol Med Rep 13: 1653-1660, 2016.
APA
Nascimento, F.P., Cardoso, M.G., Lindsey, S.C., Kunii, I.S., Valente, F.O., Kizys, M.M. ... Dias‑Da‑Silva, M.R. (2016). Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma. Molecular Medicine Reports, 13, 1653-1660. https://doi.org/10.3892/mmr.2015.4731
MLA
Nascimento, F. P., Cardoso, M. G., Lindsey, S. C., Kunii, I. S., Valente, F. O., Kizys, M. M., Delcelo, R., Camacho, C. P., Maciel, R. M., Dias‑Da‑Silva, M. R."Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma". Molecular Medicine Reports 13.2 (2016): 1653-1660.
Chicago
Nascimento, F. P., Cardoso, M. G., Lindsey, S. C., Kunii, I. S., Valente, F. O., Kizys, M. M., Delcelo, R., Camacho, C. P., Maciel, R. M., Dias‑Da‑Silva, M. R."Analysis of somatic mutations in braf, CDKN2A/p16 and PI3KCA in patients with medullary thyroid carcinoma". Molecular Medicine Reports 13, no. 2 (2016): 1653-1660. https://doi.org/10.3892/mmr.2015.4731
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