Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
February-2016 Volume 13 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2016 Volume 13 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species

  • Authors:
    • Ghada Al‑Kafaji
    • Mohamed Abdalla Sabry
    • Moiz Bakhiet
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Medicine, College of Medicine and Medical Sciences, Arabian Gulf University, Manama 26671, Bahrain, Department of Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University, Manama 26671, Bahrain
  • Pages: 1774-1780
    |
    Published online on: December 29, 2015
       https://doi.org/10.3892/mmr.2015.4732
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Reactive oxygen species (ROS)‑mediated disruption of mitochondrial respiratory function has been implicated in the complications of diabetes. The present study examined changes in the gene expression of mistochondrial DNA (mtDNA)‑encoded subunits of electron transport chain complexes in response to high glucose‑induced ROS overproduction in an in vitro model of diabetic nephropathy using human renal mesangial cells. Mitochondrial ROS generation was assessed by confocal microscopy and flow cytometry in the cells following culture in 5 and 25 mM glucose. The mRNA expression levels of nicotinamide adenine dinucleotide dehydrogenase 2 (ND2) of complex I, cytochrome b (CYTB) of complex III, cytochrome c oxidase (COI) of complex IV and ATPase 6 of complex V were analyzed by reverse transcription‑quantitative polymerase chain reaction. The protein expression levels of ND2, CYTB, COI and ATPase 6 were analyzed by western blotting. A significant increase in mitochondrial ROS production was observed in the cells cultured in 25 mM glucose, compared with cells cultured in 5 mM glucose (P<0.05). The mRNA expression of ND2, CYTB, CO1 and ATPase 6 was significantly increased following culture in 25 mM glucose, compared with the cells cultured in 5 mM glucose (P<0.05). This increase in mRNA expression was accompanied by significant increases in protein expression following incubation in 25 mM glucose (P<0.05). The increase in mtDNA‑encoded gene expression in the electron transport subunits following exposure to high glucose‑induced ROS may be a compensatory response mechanism for the decline in mitochondrial function, which may be important in the development of diabetic nephropathy through enhanced ROS generation.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Brownlee M: Biochemistry and molecular cell biology of diabetic complications. Nature. 414:813–20. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Nohl H: Generation of superoxide radicals as byproduct of cellular respiration. Ann Biol Clin (Paris). 52:199–204. 1994.

3 

Turrens JF: Superoxide production by the mitochondrial respiratory chain. Biosci Rep. 17:3–8. 1997. View Article : Google Scholar : PubMed/NCBI

4 

Nishikawa T, Edelstein D, Du XL, Yamagishi S, Matsumura T, Kaneda Y, Yorek MA, Beebe D, Oates PJ, Hammes HP, et al: Normalizing mitochondrial superoxide production blocks three different pathways of hyperglycemic damage. Nature. 404:787–790. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, et al: Sequence and organization of the human mitochondrial genome. Nature. 290:457–65. 1981. View Article : Google Scholar : PubMed/NCBI

6 

Taanman JW: The mitochondrial genome: Structure, transcription, translation and replication. Biochim Biophys Acta. 1410:103–123. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Bohr VA: Repair of oxidative DNA damage in nuclear and mitochondrial DNA, and some changes with aging in mammalian cells. Free Radic Biol Med. 32:804–812. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Santos JH, Hunakova L, Chen Y, Bortner C and Van Houten B: Cell sorting experiments link persistent mitochondrial DNA damage with loss of mitochondrial membrane potential and apoptotic cell death. J Biol Chem. 278:1728–1734. 2003. View Article : Google Scholar

9 

Kirkinezos IG and Moraes CT: Reactive oxygen species and mitochondrial diseases. Semin Cell Dev Biol. 12:449–457. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Gu XM, Huang HC and Jiang ZF: Mitochondrial dysfunction and cellular metabolic deficiency in Alzheimer's disease. Neurosci Bull. 28:631–640. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Hauser DN and Hastings TG: Mitochondrial dysfunction and oxidative stress in Parkinson's disease and monogenic parkinsonism. Neurobiol Dis. 51:35–42. 2013. View Article : Google Scholar :

12 

Boland ML, Chourasia AH and Macleod KF: Mitochondrial dysfunction in cancer. Front Oncol. 3:2922013. View Article : Google Scholar : PubMed/NCBI

13 

Manczak M, Jung Y, Park BS, Partovi D and Reddy PH: Time-course of mitochondrial gene expressions in mice brains: Implications for mitochondrial dysfunction, oxidative damage and cytochrome c in aging. J Neurochem. 92:494–504. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Lee HC and Wei YH: Mitochondrial role in life and death of the cell. J Biomed Sci. 7:2–15. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Raza H, Prabu SK, John A and Avadhani NG: Impaired mitochondrial respiratory functions and oxidative stress in strep-tozotocin-induced diabetic rats. Int J Mol Sci. 12:3133–3147. 2011. View Article : Google Scholar

16 

Rolo AP and Palmeira CM: Diabetes and mitochondrial function: Role of hyperglycemia and oxidative stress. Toxicol Appl Pharmacol. 212:167–178. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Munusamy S, Saba H, Mitchell T, Megyesi JK, Brock RW and MacMillan-Crow LA: Alteration of renal respiratory Complex-III during experimental type-1 diabetes. BMC Endocr Disord. 9:22009. View Article : Google Scholar : PubMed/NCBI

18 

Al-Kafaji G and Golbahar J: High glucose-induced oxidative stress increases the copy number of mitochondrial DNA in human mesangial cells. Biomed Res Int. 2013:7549462013. View Article : Google Scholar : PubMed/NCBI

19 

Al-Kafaji G and Malik AN: Hyperglycemia induces elevated expression of thyroid hormone binding protein in vivo in kidney and heart and in vitro in mesangial cells. Biochem Biophys Res Commun. 391:1585–1591. 2010. View Article : Google Scholar

20 

Malik AN and Al-Kafaji G: Glucose regulation of beta-defensin-1 mRNA in human renal cells. Biochem Biophys Res Commun. 353:318–323. 2007. View Article : Google Scholar

21 

Al-Kafaji G, Sabry MA and Skrypnyk C: Time-course effect of high-glucose-induced reactive oxygen species on mitochondrial biogenesis and function in human renal mesangial cells. Cell Biol Int. Aug 6–2015.Epub ahead of print. PubMed/NCBI

22 

Murphy MP: How mitochondria produce reactive oxygen species. Biochem J. 417:1–13. 2009. View Article : Google Scholar

23 

Esposito LA, Melov S, Panov A, Cottrell BA and Wallace DC: Mitochondrial disease in mouse results in increased oxidative stress. Proc Natl Acad Sci USA. 96:4820–4825. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Enns GM: The contribution of mitochondria to common disorders. Mol Genet Metab. 80:11–26. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Lee HC and Wei YH: Mitochondrial biogenesis and mitochondrial DNA maintenance of mammalian cells under oxidative stress. Int J Biochem Cell Biol. 37:822–834. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Ahuja P, Wanagat J, Wang Z, Wang Y, Liem DA, Ping P, Antoshechkin IA, Margulies KB and MacLellan WR: Divergent mitochondrial biogenesis responses in human cardiomyopathy. Circulation. 127:1957–1967. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Antonetti DA, Reynet C and Kahn CR: Increased expression of mitochondrial-encoded genes in skeletal muscle of humans with diabetes mellitus. J Clin Invest. 95:1383–1388. 1995. View Article : Google Scholar : PubMed/NCBI

28 

Ballinger SW, Patterson C, Yan CN, Doan R, Burow DL, Young CG, Yakes FM, Van Houten B, Ballinger CA, Freeman BA and Runge MS: Hydrogen peroxide- and peroxynitrite-induced mitochondrial DNA damage and dysfunction in vascular endothelial and smooth muscle cells. Circ Res. 86:960–966. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Madsen-Bouterse SA, Zhong Q, Mohammad G, Ho YS and Kowluru RA: Oxidative damage of mitochondrial DNA in diabetes and its protection by manganese superoxide dismutase. Free Radic Res. 44:313–321. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Santos JM, Tewari S, Goldberg AF and Kowluru RA: Mitochondria biogenesis and the development of diabetic retinopathy. Free Radic Biol Med. 51:1849–1860. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Ide T, Tsutsui H, Hayashidani S, Kang D, Suematsu N, Nakamura K, Utsumi H, Hamasaki N and Takeshita A: Mitochondrial DNA damage and dysfunction associated with oxidative stress in failing hearts after myocardial infarction. Circ Res. 88:529–535. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Xie L, Zhu X, Hu Y, Li T, Gao Y, Shi Y and Tang S: Mitochondrial DNA oxidative damage triggering mitochondrial dysfunction and apoptosis in high glucose-induced HRECs. Invest Ophthalmol Vis Sci. 49:4203–4209. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Al‑Kafaji G, Sabry MA and Bakhiet M: Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species. Mol Med Rep 13: 1774-1780, 2016.
APA
Al‑Kafaji, G., Sabry, M.A., & Bakhiet, M. (2016). Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species. Molecular Medicine Reports, 13, 1774-1780. https://doi.org/10.3892/mmr.2015.4732
MLA
Al‑Kafaji, G., Sabry, M. A., Bakhiet, M."Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species". Molecular Medicine Reports 13.2 (2016): 1774-1780.
Chicago
Al‑Kafaji, G., Sabry, M. A., Bakhiet, M."Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species". Molecular Medicine Reports 13, no. 2 (2016): 1774-1780. https://doi.org/10.3892/mmr.2015.4732
Copy and paste a formatted citation
x
Spandidos Publications style
Al‑Kafaji G, Sabry MA and Bakhiet M: Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species. Mol Med Rep 13: 1774-1780, 2016.
APA
Al‑Kafaji, G., Sabry, M.A., & Bakhiet, M. (2016). Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species. Molecular Medicine Reports, 13, 1774-1780. https://doi.org/10.3892/mmr.2015.4732
MLA
Al‑Kafaji, G., Sabry, M. A., Bakhiet, M."Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species". Molecular Medicine Reports 13.2 (2016): 1774-1780.
Chicago
Al‑Kafaji, G., Sabry, M. A., Bakhiet, M."Increased expression of mitochondrial DNA‑encoded genes in human renal mesangial cells in response to high glucose‑induced reactive oxygen species". Molecular Medicine Reports 13, no. 2 (2016): 1774-1780. https://doi.org/10.3892/mmr.2015.4732
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team