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Article

Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression

  • Authors:
    • Ziye Wang
    • Wenwen Li
    • Jin Chen
    • Haiyang Shi
    • Mingjun Zhao
    • Hongmin You
    • Chenglong Rao
    • Yuan Zhan
    • Yongtao Yang
    • Peng Xie
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, P.R. China, Chongqing Key Laboratory of Neurobiology, Chongqing 400016, P.R. China
  • Pages: 1813-1820
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    Published online on: December 30, 2015
       https://doi.org/10.3892/mmr.2015.4741
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Abstract

Substantial evidence from previous studies has suggested an association between major depressive disorder (MDD) and inflammation, and previous studies have associated prefrontal cortex (PFC) dysfunction with MDD. Systemic administration of bacterial lipopolysaccharide has been used to study inflammation‑associated behavioral changes in rodents. However, proteomic studies investigating PFC protein expression in an LPS‑induced mouse model of depression have yet to be conducted. Using two‑dimensional electrophoresis coupled with matrix‑assisted laser desorption ionization‑time of flight‑tandem mass spectrometry, PFC proteomes were comparatively assessed in LPS‑induced acute inflammation reaction mice, LPS‑induced depressive‑like behavior mice (Dep), and control mice. A total of 26 differentially expressed proteins were identified, two of which were selected for western blot analysis, the results of which revealed a significant increase in the expression levels of creatine kinase B and dihydropyrimidinase‑like 3 in Dep mice, suggesting that changes in energy metabolism and neuro­genesis occur in the PFC of Dep mice. Further investigation on these processes and on the proteins of the PFC are required in order to elucidate the pathophysiological mechanism underlying MDD.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Li W, Chen J, Shi H, Zhao M, You H, Rao C, Zhan Y, Yang Y, Xie P, Xie P, et al: Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression. Mol Med Rep 13: 1813-1820, 2016.
APA
Wang, Z., Li, W., Chen, J., Shi, H., Zhao, M., You, H. ... Xie, P. (2016). Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression. Molecular Medicine Reports, 13, 1813-1820. https://doi.org/10.3892/mmr.2015.4741
MLA
Wang, Z., Li, W., Chen, J., Shi, H., Zhao, M., You, H., Rao, C., Zhan, Y., Yang, Y., Xie, P."Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression". Molecular Medicine Reports 13.2 (2016): 1813-1820.
Chicago
Wang, Z., Li, W., Chen, J., Shi, H., Zhao, M., You, H., Rao, C., Zhan, Y., Yang, Y., Xie, P."Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression". Molecular Medicine Reports 13, no. 2 (2016): 1813-1820. https://doi.org/10.3892/mmr.2015.4741
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Li W, Chen J, Shi H, Zhao M, You H, Rao C, Zhan Y, Yang Y, Xie P, Xie P, et al: Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression. Mol Med Rep 13: 1813-1820, 2016.
APA
Wang, Z., Li, W., Chen, J., Shi, H., Zhao, M., You, H. ... Xie, P. (2016). Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression. Molecular Medicine Reports, 13, 1813-1820. https://doi.org/10.3892/mmr.2015.4741
MLA
Wang, Z., Li, W., Chen, J., Shi, H., Zhao, M., You, H., Rao, C., Zhan, Y., Yang, Y., Xie, P."Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression". Molecular Medicine Reports 13.2 (2016): 1813-1820.
Chicago
Wang, Z., Li, W., Chen, J., Shi, H., Zhao, M., You, H., Rao, C., Zhan, Y., Yang, Y., Xie, P."Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide‑induced mouse model of depression". Molecular Medicine Reports 13, no. 2 (2016): 1813-1820. https://doi.org/10.3892/mmr.2015.4741
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