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Article Open Access

Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke

  • Authors:
    • Weilin Liu
    • Xian Wang
    • Yi Zheng
    • Guanhao Shang
    • Jia Huang
    • Jing Tao
    • Lidian Chen
  • View Affiliations / Copyright

    Affiliations: College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Fujian Rehabilitation Tech Co‑innovation Center, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Fujian Rehabilitation Engineering Research Center & Fujian Key Lab of Motor Function Rehabilitation, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1618-1626
    |
    Published online on: December 30, 2015
       https://doi.org/10.3892/mmr.2015.4745
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Abstract

The aim of the present study was to investigate the neuroprotective mechanism of the miR‑9‑mediated activation of the nuclear factor (NF)‑κB signaling pathway by electroacupuncture (EA) stimulation of the Quchi (LI11) and Zusanli (ST36) acupoints in a rat model of middle cerebral artery occlusion (MCAO). The present study demonstrated that EA alleviated the symptoms of neurological deficits and reduced the infarct volume in the rat brains. The expression of miR‑9 in the peri‑infarct cortex was increased in the EA group compared with the MCAO group, and the expression of NF‑κB signaling pathway‑associated factors, NF‑κB p65, tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β were reduced. Notably, miR‑9 inhibitors were revealed to have the ability to suppress EA‑alleviated cerebral inflammation and the expression of NF‑κB downstream‑related factors, NF‑κB p65, TNF‑α and IL‑1β, and caused no alteration on the level of NF‑κB upstream‑related protein inhibitor of κBα, suggesting that the cerebral protective efficacy of EA targets miR‑9‑mediated NF‑κB downstream pathway following ischemic stroke.
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1 

Cullen BR: Transcription and processing of human microRNA precursors. Mol Cell. 16:861–865. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Doench JG and Sharp PA: Specificity of microRNA target selection in translational repression. Genes Dev. 18:504–511. 2004. View Article : Google Scholar : PubMed/NCBI

3 

O'Connell RM, Rao DS and Baltimore D: MicroRNA regulation of inflammatory responses. Annu Rev Immunol. 30:295–312. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Akerblom M, Sachdeva R, Quintino L, Wettergren EE, Chapman KZ, Manfre G, Lindvall O, Lundberg C and Jakobsson J: Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9. Nat Commun. 4:17702013. View Article : Google Scholar : PubMed/NCBI

5 

Yao H, Ma R, Yang L, Hu G, Chen X, Duan M, Kook Y, Niu F, Liao K, Fu M, et al: MiR-9 promotes microglial activation by targeting MCPIP1. Nat Commun. 5:43862014. View Article : Google Scholar : PubMed/NCBI

6 

Guo LM, Pu Y, Han Z, Liu T, Li YX, Liu M, Li X and Tang H: MicroRNA-9 inhibits ovarian cancer cell growth through regulation of NF-kappaB1. FEBS J. 276:5537–5546. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Wang J, Gu Z, Ni P, Qiao Y, Chen C, Liu X, Lin J, Chen N and Fan Q: NF-kappaB P50/P65 hetero-dimer mediates differential regulation of CD166/ALCAM expression via interaction with micoRNA-9 after serum deprivation, providing evidence for a novel negative auto-regulatory loop. Nucleic Acids Res. 39:6440–6455. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Lukiw WJ: NF-κB-regulated micro RNAs (miRNAs) in primary human brain cells. Exp Neurol. 235:484–490. 2012. View Article : Google Scholar :

9 

Liu XS, Chopp M, Zhang RL, Tao T, Wang XL, Kassis H, Hozeska-Solgot A, Zhang L, Chen C and Zhang ZG: MicroRNA profiling in subventricular zone after stroke: MiR-124a regulates proliferation of neural progenitor cells through Notch signaling pathway. PLoS One. 6:e234612011. View Article : Google Scholar : PubMed/NCBI

10 

Liu Y, Zhang J, Han R, Liu H, Sun D and Liu X: Downregulation of serum brain specific microRNA is associated with inflammation and infarct volume in acute ischemic stroke. J Clin Neurosci. 22:291–295. 2015. View Article : Google Scholar

11 

Chen W, Gu HW, Ma WP, Li QS, Yu Q, Liu XQ, Liu SH, Li WH, Liu HL and Dai MT: Multicentral randomized controlled study on effects of acupuncture at Zusanli (ST 36) and Xuanzhong (GB 39) on cerebrovascular function in the patient of ischemic stroke. Zhongguo Zhen Jiu. 26:851–853. 2006.In Chinese.

12 

Lan L, Tao J, Chen A, Xie G, Huang J, Lin J, Peng J and Chen L: Electroacupuncture exerts anti-inflammatory effects in cerebral ischemia-reperfusion injured rats via suppression of the TLR4/NF-κB pathway. Int J Mol Med. 31:75–80. 2013.

13 

Jin Z, Liang J, Wang J and Kolattukudy PE: Delayed brain ischemia tolerance induced by electroacupuncture pretreatment is mediated via MCP-induced protein 1. J Neuroinflammation. 10:632013. View Article : Google Scholar : PubMed/NCBI

14 

Xue X, You Y, Tao J, Ye X, Huang J, Yang S, Lin Z, Hong Z, Peng J and Chen L: Electro-acupuncture at points of Zusanli and Quchi exerts anti-apoptotic effect through the modulation of PI3K/Akt signaling pathway. Neurosci Lett. 558:14–19. 2014. View Article : Google Scholar

15 

Lu T, Song QH, Xu RM, Guo YH, Wang F, Hu JP, Wang Y and Zhang LY: Dance combined with magnetic pulse stimulates the ability of walk and balance in elder people. Int J Clin Exp Med. 8:4381–4386. 2015.PubMed/NCBI

16 

Zhao H, Wang J, Gao L, Wang R, Liu X, Gao Z, Tao Z, Xu C, Song J, Ji X and Luo Y: MiRNA-424 protects against permanent focal cerebral ischemia injury in mice involving suppressing microglia activation. Stroke. 44:1706–1713. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI

18 

Zhuang L, He J, Zhuang X and Lu L: Quality of reporting on randomized controlled trials of acupuncture for stroke rehabilitation. BMC Complement Altern Med. 14:1512014. View Article : Google Scholar : PubMed/NCBI

19 

Chang L, He PL, Zhou ZZ and Li YH: Efficacy observation of dysphagia after acute stroke treated with acupuncture and functional electric stimulation. Zhongguo Zhen Jiu. 34:737–740. 2014.In Chinese. PubMed/NCBI

20 

Sze FK, Wong E, Or KK, Lau J and Woo J: Does acupuncture improve motor recovery after stroke? A meta-analysis of randomized controlled trials. Stroke. 33:2604–2619. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Shen PF, Kong L, Ni LW, Guo HL, Yang S, Zhang LL, Zhang ZL, Guo JK, Xiong J, Zhen Z, et al: Acupuncture intervention in ischemic stroke: A randomized controlled prospective study. Am J Chin Med. 40:685–693. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Wang H, Xie Y, Zhang Q, Xu N, Zhong H, Dong H, Liu L, Jiang T, Wang Q and Xiong L: Transcutaneous electric acupoint stimulation reduces intra-operative remifentanil consumption and alleviates postoperative side-effects in patients undergoing sinusotomy: A prospective, randomized, placebo-controlled trial. Br J Anaesth. 112:1075–1082. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Harari OA and Liao JK: NF-κB and innate immunity in ischemic stroke. Ann N Y Acad Sci. 1207:32–40. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Feng X, Yang S, Liu J, Huang J, Peng J, Lin J, Tao J and Chen L: Electroacupuncture ameliorates cognitive impairment through inhibition of NF-κB-mediated neuronal cell apoptosis in cerebral ischemia-reperfusion injured rats. Mol Med Rep. 7:1516–1522. 2013.PubMed/NCBI

25 

Wang ZK, Ni GX, Liu K, Xiao ZX, Yang BW, Wang J and Wang S: Research on the changes of IL-1 receptor and TNF-alpha receptor in rats with cerebral ischemia reperfusion and the chronergy of acupuncture intervention. Zhongguo Zhen Jiu. 32:1012–1018. 2012.In Chinese. PubMed/NCBI

26 

Alvarez-Garcia I and Miska EA: MicroRNA functions in animal development and human disease. Development. 132:4653–4662. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Ambros V: MicroRNA pathways in flies and worms: Growth, death, fat, stress and timing. Cell. 113:673–676. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Quinn SR and O'Neill LA: A trio of microRNAs that control Toll-like receptor signalling. Int Immunol. 23:421–425. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Sheedy FJ, Palsson-McDermott E, Hennessy EJ, Martin C, O'Leary JJ, Ruan Q, Johnson DS, Chen Y and O'Neill LA: Negative regulation of TLR4 via targeting of the proinflammatory tumor suppressor PDCD4 by the microRNA miR-21. Nat Immunol. 11:141–147. 2010. View Article : Google Scholar

30 

El Gazzar M and McCall CE: MicroRNAs distinguish translational from transcriptional silencing during endotoxin tolerance. J Biol Chem. 285:20940–20951. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Tili E, Michaille JJ, Cimino A, Costinean S, Dumitru CD, Adair B, Fabbri M, Alder H, Liu CG, Calin GA and Croce CM: Modulation of miR-155 and miR-125b levels following lipopolysaccharide/TNF-alpha stimulation and their possible roles in regulating the response to endotoxin shock. J Immunol. 179:5082–5089. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Liu DZ, Tian Y, Ander BP, Xu H, Stamova BS, Zhan X, Turner RJ, Jickling G and Sharp FR: Brain and blood microRNA expression profiling of ischemic stroke, intracerebral hemorrhage and kainate seizures. J Cereb Blood Flow Metab. 30:92–101. 2010. View Article : Google Scholar

33 

Thulin P, Wei T, Werngren O, Cheung L, Fisher RM, Grandér D, Corcoran M and Ehrenborg E: MicroRNA-9 regulates the expression of peroxisome proliferator-activated receptor δ in human monocytes during the inflammatory response. Int J Mol Med. 31:1003–1010. 2013.PubMed/NCBI

34 

Bazzoni F, Rossato M, Fabbri M, Gaudiosi D, Mirolo M, Mori L, Tamassia N, Mantovani A, Cassatella MA and Locati M: Induction and regulatory function of miR-9 in human monocytes and neutrophils exposed to proinflammatory signals. Proc Natl Acad Sci USA. 106:5282–5287. 2009. View Article : Google Scholar : PubMed/NCBI

35 

Delaloy C and Gao FB: MicroRNA-9 multitasking near organizing centers. Nat Neurosci. 11:625–626. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Coolen M and Bally-Cuif L: MicroRNAs in brain development and physiology. Curr Opin Neurobiol. 19:461–470. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Wan HY, Guo LM, Liu T, Liu M, Li X and Tang H: Regulation of the transcription factor NF-kappaB1 by microRNA-9 in human gastric adenocarcinoma. Mol Cancer. 9:162010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu W, Wang X, Zheng Y, Shang G, Huang J, Tao J and Chen L: Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke. Mol Med Rep 13: 1618-1626, 2016.
APA
Liu, W., Wang, X., Zheng, Y., Shang, G., Huang, J., Tao, J., & Chen, L. (2016). Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke. Molecular Medicine Reports, 13, 1618-1626. https://doi.org/10.3892/mmr.2015.4745
MLA
Liu, W., Wang, X., Zheng, Y., Shang, G., Huang, J., Tao, J., Chen, L."Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke". Molecular Medicine Reports 13.2 (2016): 1618-1626.
Chicago
Liu, W., Wang, X., Zheng, Y., Shang, G., Huang, J., Tao, J., Chen, L."Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke". Molecular Medicine Reports 13, no. 2 (2016): 1618-1626. https://doi.org/10.3892/mmr.2015.4745
Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Wang X, Zheng Y, Shang G, Huang J, Tao J and Chen L: Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke. Mol Med Rep 13: 1618-1626, 2016.
APA
Liu, W., Wang, X., Zheng, Y., Shang, G., Huang, J., Tao, J., & Chen, L. (2016). Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke. Molecular Medicine Reports, 13, 1618-1626. https://doi.org/10.3892/mmr.2015.4745
MLA
Liu, W., Wang, X., Zheng, Y., Shang, G., Huang, J., Tao, J., Chen, L."Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke". Molecular Medicine Reports 13.2 (2016): 1618-1626.
Chicago
Liu, W., Wang, X., Zheng, Y., Shang, G., Huang, J., Tao, J., Chen, L."Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke". Molecular Medicine Reports 13, no. 2 (2016): 1618-1626. https://doi.org/10.3892/mmr.2015.4745
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