Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke

Liu,Weilin; Wang,Xian; Zheng,Yi; Shang,Guanhao; Huang,Jia; Tao,Jing; Chen,Lidian

doi:10.3892/mmr.2015.4745

Electroacupuncture inhibits inflammatory injury by targeting the miR‑9‑mediated NF‑κB signaling pathway following ischemic stroke

Authors:
- Weilin Liu
- Xian Wang
- Yi Zheng
- Guanhao Shang
- Jia Huang
- Jing Tao
- Lidian Chen
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Affiliations: College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Fujian Rehabilitation Tech Co‑innovation Center, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Fujian Rehabilitation Engineering Research Center & Fujian Key Lab of Motor Function Rehabilitation, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
Published online on: December 30, 2015 https://doi.org/10.3892/mmr.2015.4745
Pages: 1618-1626
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the neuroprotective mechanism of the miR‑9‑mediated activation of the nuclear factor (NF)‑κB signaling pathway by electroacupuncture (EA) stimulation of the Quchi (LI11) and Zusanli (ST36) acupoints in a rat model of middle cerebral artery occlusion (MCAO). The present study demonstrated that EA alleviated the symptoms of neurological deficits and reduced the infarct volume in the rat brains. The expression of miR‑9 in the peri‑infarct cortex was increased in the EA group compared with the MCAO group, and the expression of NF‑κB signaling pathway‑associated factors, NF‑κB p65, tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β were reduced. Notably, miR‑9 inhibitors were revealed to have the ability to suppress EA‑alleviated cerebral inflammation and the expression of NF‑κB downstream‑related factors, NF‑κB p65, TNF‑α and IL‑1β, and caused no alteration on the level of NF‑κB upstream‑related protein inhibitor of κBα, suggesting that the cerebral protective efficacy of EA targets miR‑9‑mediated NF‑κB downstream pathway following ischemic stroke.

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