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TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells

  • Authors:
    • Qingjie Wang
    • Dong Wang
    • Gaoling Yan
    • Ling Sun
    • Chengchun Tang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Zhongda Hospital of Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Cardiology, Changzhou Hospital of Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1577-1585
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    Published online on: December 30, 2015
       https://doi.org/10.3892/mmr.2015.4750
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Abstract

Hypoxia induces pulmonary vasoconstriction and reconstruction in the pulmonary arteries and pulmonary veins (PVs), and elevation of intracellular calcium concentration ([Ca2+]i) is a primary factor of these processes. In the present study, the role of transient receptor potential cation channels (TRPCs) in mediating the hypoxia‑induced elevation of [Ca2+]i in rat distal pulmonary venous smooth muscle cells (PVSMCs) was investigated. Rats with chronic hypoxic pulmonary hypertension (CHPH) were used for in vivo experiments, and PVSMCs were isolated for in vitro experiments. [Ca2+]i was measured using fura‑2‑based fluorescence calcium imaging. Reverse transcription‑quantitative polymerase chain reaction and western blotting were used to detect the mRNA and protein expression levels of TRPCs. Methyl thiazolyl tetrazolium and Transwell assays were used to investigate the proliferation and migration of PVSMCs, respectively. The results of the present study demonstrated that TRPC6 was increased in the distal PVs of CHPH rats, and in PVSMCs exposed to hypoxic conditions (4% O2, 72 h); however, TRPC1 was not. The 1‑oleoyl-2‑acetyl-sn-glycerol-induced [Ca2+]i elevation was increased in PVSMCs isolated from CHPH rats and in PVSMCs cultured under hypoxic conditions (4% O2, 72 h). Hypoxia induced PVSMC [Ca2+]i elevation, proliferation and migration. These alterations were inhibited following TRPC6 knockdown. Results from the present study suggest that TRPC6 expression is increased during chronic hypoxia, which contributes to Ca2+ entry into the cell, thus promoting proliferation and migration of PVSMCs.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Q, Wang D, Yan G, Sun L and Tang C: TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells. Mol Med Rep 13: 1577-1585, 2016.
APA
Wang, Q., Wang, D., Yan, G., Sun, L., & Tang, C. (2016). TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells. Molecular Medicine Reports, 13, 1577-1585. https://doi.org/10.3892/mmr.2015.4750
MLA
Wang, Q., Wang, D., Yan, G., Sun, L., Tang, C."TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells". Molecular Medicine Reports 13.2 (2016): 1577-1585.
Chicago
Wang, Q., Wang, D., Yan, G., Sun, L., Tang, C."TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells". Molecular Medicine Reports 13, no. 2 (2016): 1577-1585. https://doi.org/10.3892/mmr.2015.4750
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Q, Wang D, Yan G, Sun L and Tang C: TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells. Mol Med Rep 13: 1577-1585, 2016.
APA
Wang, Q., Wang, D., Yan, G., Sun, L., & Tang, C. (2016). TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells. Molecular Medicine Reports, 13, 1577-1585. https://doi.org/10.3892/mmr.2015.4750
MLA
Wang, Q., Wang, D., Yan, G., Sun, L., Tang, C."TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells". Molecular Medicine Reports 13.2 (2016): 1577-1585.
Chicago
Wang, Q., Wang, D., Yan, G., Sun, L., Tang, C."TRPC6 is required for hypoxia‑induced basal intracellular calcium concentration elevation, and for the proliferation and migration of rat distal pulmonary venous smooth muscle cells". Molecular Medicine Reports 13, no. 2 (2016): 1577-1585. https://doi.org/10.3892/mmr.2015.4750
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