Stimulus-dependent neuronal cell responses in SH-SY5Y neuroblastoma cells

Sun,Xiguang; Shi,Xu; Lu,Laijing; Jiang,Yanfang; Liu,Bin

doi:10.3892/mmr.2016.4759

March-2016 Volume 13 Issue 3

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March-2016 Volume 13 Issue 3

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Article

Stimulus-dependent neuronal cell responses in SH-SY5Y neuroblastoma cells

Authors:
- Xiguang Sun
- Xu Shi
- Laijing Lu
- Yanfang Jiang
- Bin Liu
View Affiliations / Copyright

Affiliations: Department of Hand and Foot Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Central Laboratory, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Pages: 2215-2220
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Published online on: January 12, 2016

https://doi.org/10.3892/mmr.2016.4759
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Abstract

The aim of the present study was to elucidate the intracellular mechanisms that cause neuronal cell death following exposure to excitatory neurotransmitter‑induced neurotoxicity, neurotoxins and oxidative stress. Human SH‑SY5Y neuroblastoma cells were exposed to various stimuli, including glutamate, 6‑hydroxydopamine (6‑OHDA), and glucose oxidase, and cell viability was determined by MTT assay. Early apoptosis and necrosis were examined by Annexin V/propidium iodide double staining and flow cytometric analysis. Intracellular calcium ion concentration and mitochondrial membrane potential were assessed by Fluo‑3a and JC‑1 staining, respectively. In addition, protein expression of receptor‑interacting protein (RIP) kinase 1 and RIP kinase 3 were evaluated by western blotting. Glutamate, 6‑OHDA and glucose oxidase treatment decreased cell viability. Glutamate induced apoptosis and necrosis, whereas, 6‑OHDA induced cell necrosis and glucose oxidase induced apoptosis. Furthermore, glutamate, 6‑OHDA or glucose oxidase treatment significantly increased intracellular calcium concentrations (P<0.05). The effect of glutamate on mitochondrial membrane potential varied with high and low concentrations, whereas 6‑OHDA and glucose oxidase significantly increased the mitochondrial membrane potential in the SH‑SY5Y cells (P<0.05). Glutamate significantly upregulated expression levels of RIP kinase 1 (P<0.05), but not RIP kinase 3. These findings demonstrate that the response of SH‑SY5Y cells varies with the stimuli. Furthermore, RIP kinase 1 may specifically regulate programmed necrosis in glutamate‑mediated excitatory toxicity, but not in cell damage induced by either 6-OHDA or glucose oxidase.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Stimulus-dependent neuronal cell responses in SH-SY5Y neuroblastoma cells

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