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Article Open Access

Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis

  • Authors:
    • Taimao Jiang
    • Tao Liu
    • Lin Li
    • Zhijun Yang
    • Yunfeng Bai
    • Dongye Liu
    • Chuize Kong
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Rehabilitation Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110036, P.R. China, Department of Neurosurgery, Hospital of Beijing Military District of People's Liberation Army, Beijing 100070, P.R. China, Infection Section 15, The 302 Hospital of Chinese People's Liberation Army, Beijing 100039, P.R. China, Department of Urology, The 463 Hospital of Chinese People's Liberation Army, Shenyang, Liaoning 110042, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2039-2045
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    Published online on: January 12, 2016
       https://doi.org/10.3892/mmr.2016.4762
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Abstract

Bladder cancer frequently shows mutational activation of the oncogene Ras, which is associated with bladder carcinogenesis. However, the signaling pathway downstream of Ras remains to be fully elucidated. N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) is able to induce bladder cancer by driving the clonal expansion of initiated cells carrying the activated form of Ras. Phospholipase Cε (PLCε) is the main target of BBN, while the tumor promoting role of PLCε remains controversial. The present study examined the role of PLCε in BBN‑induced bladder carcinogenesis of mice with genetically inactivated PLCε. Using light and electron microscopy, the present study demonstrated that PLCε‑/‑ mice were resistant to BBN‑induced bladder carcinogenesis. Furthermore, it was demonstrated that cyclooxygenase 2 and vascular endothelial growth factor‑A were affected by the PLCε background of the mice, suggesting that the role of PLCε in tumor promotion may be ascribed to augmentation of inflammatory responses and angiogenesis. These results indicated that PLCε is crucial for BBN‑induced bladder carcinogenesis as well as signaling downstream of Ras, and that PLCε is a candidate molecular target for the development of anti-cancer drugs.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang T, Liu T, Li L, Yang Z, Bai Y, Liu D and Kong C: Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis. Mol Med Rep 13: 2039-2045, 2016.
APA
Jiang, T., Liu, T., Li, L., Yang, Z., Bai, Y., Liu, D., & Kong, C. (2016). Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis. Molecular Medicine Reports, 13, 2039-2045. https://doi.org/10.3892/mmr.2016.4762
MLA
Jiang, T., Liu, T., Li, L., Yang, Z., Bai, Y., Liu, D., Kong, C."Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis". Molecular Medicine Reports 13.3 (2016): 2039-2045.
Chicago
Jiang, T., Liu, T., Li, L., Yang, Z., Bai, Y., Liu, D., Kong, C."Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis". Molecular Medicine Reports 13, no. 3 (2016): 2039-2045. https://doi.org/10.3892/mmr.2016.4762
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang T, Liu T, Li L, Yang Z, Bai Y, Liu D and Kong C: Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis. Mol Med Rep 13: 2039-2045, 2016.
APA
Jiang, T., Liu, T., Li, L., Yang, Z., Bai, Y., Liu, D., & Kong, C. (2016). Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis. Molecular Medicine Reports, 13, 2039-2045. https://doi.org/10.3892/mmr.2016.4762
MLA
Jiang, T., Liu, T., Li, L., Yang, Z., Bai, Y., Liu, D., Kong, C."Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis". Molecular Medicine Reports 13.3 (2016): 2039-2045.
Chicago
Jiang, T., Liu, T., Li, L., Yang, Z., Bai, Y., Liu, D., Kong, C."Knockout of phospholipase Cε attenuates N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder tumorigenesis". Molecular Medicine Reports 13, no. 3 (2016): 2039-2045. https://doi.org/10.3892/mmr.2016.4762
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