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Article

Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression

  • Authors:
    • Yan Ma
    • Zhi Shan
    • Jianjun Ma
    • Qiang Wang
    • Junjie Chu
    • Peiwei Xu
    • An Qin
    • Shunwu Fan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China, Department of Orthopaedics, Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, P.R. China
  • Pages: 2273-2280
    |
    Published online on: January 12, 2016
       https://doi.org/10.3892/mmr.2016.4765
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Abstract

Enhanced osteoclast formation and function have essential roles during post‑menopausal osteoporosis. A number of cytokines have been reported to regulate osteoclastogenesis and to be involved during the pathogenesis of osteoporosis. However, the regulation of osteolysis by microRNAs (miRNAs) has remained to be fully elucidated. The present study used a microarray analysis to identify a variety of miRNAs that are differentially expressed during osteoclast formation. Six down‑regulated miRNAs, miR‑21a‑5p, miR‑27a‑3p, let‑7i‑5p, miR‑22‑3p, miR‑340‑5p and miR‑23a‑5p, whose molecular mechanisms during osteoclast differentiation have not been reported previously, were further assessed. Using an osteoclast formation assay and a mouse model of progressive osteoporosis, the downregulation of these miRNAs was validated in vitro and in vivo. Of note, the expression patterns of these six miRNAs were associated with the progression of osteoporosis. Therefore, these miRNAs are of potential diagnostic and therapeutic value for osteolytic diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Shan Z, Ma J, Wang Q, Chu J, Xu P, Qin A and Fan S: Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression. Mol Med Rep 13: 2273-2280, 2016.
APA
Ma, Y., Shan, Z., Ma, J., Wang, Q., Chu, J., Xu, P. ... Fan, S. (2016). Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression. Molecular Medicine Reports, 13, 2273-2280. https://doi.org/10.3892/mmr.2016.4765
MLA
Ma, Y., Shan, Z., Ma, J., Wang, Q., Chu, J., Xu, P., Qin, A., Fan, S."Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression". Molecular Medicine Reports 13.3 (2016): 2273-2280.
Chicago
Ma, Y., Shan, Z., Ma, J., Wang, Q., Chu, J., Xu, P., Qin, A., Fan, S."Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression". Molecular Medicine Reports 13, no. 3 (2016): 2273-2280. https://doi.org/10.3892/mmr.2016.4765
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Shan Z, Ma J, Wang Q, Chu J, Xu P, Qin A and Fan S: Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression. Mol Med Rep 13: 2273-2280, 2016.
APA
Ma, Y., Shan, Z., Ma, J., Wang, Q., Chu, J., Xu, P. ... Fan, S. (2016). Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression. Molecular Medicine Reports, 13, 2273-2280. https://doi.org/10.3892/mmr.2016.4765
MLA
Ma, Y., Shan, Z., Ma, J., Wang, Q., Chu, J., Xu, P., Qin, A., Fan, S."Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression". Molecular Medicine Reports 13.3 (2016): 2273-2280.
Chicago
Ma, Y., Shan, Z., Ma, J., Wang, Q., Chu, J., Xu, P., Qin, A., Fan, S."Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression". Molecular Medicine Reports 13, no. 3 (2016): 2273-2280. https://doi.org/10.3892/mmr.2016.4765
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