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Article

Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells

  • Authors:
    • Sheng‑Qian Xu
    • Yong Qin
    • De‑Biao Pan
    • Guan‑Xiong Ye
    • Cheng‑Jun Wu
    • Shi Wang
    • Jin‑Yan Jiang
    • Jing Fu
    • Chao‑Jun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, People's Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang 323000, P.R. China
  • Pages: 2261-2266
    |
    Published online on: January 13, 2016
       https://doi.org/10.3892/mmr.2016.4771
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Abstract

Primary liver cancer is one of the most common and aggressive human malignancies worldwide. As numerous studies have revealed that WW domain containing E3 Ub‑protein ligase 2 (WWP2) exerts cancer‑specific functions, the present study assessed the role of WWP2 in liver cancer. WWP2 was revealed to be significantly overexpressed in liver cancer tissues compared with paired normal tissues at the mRNA as well as at the protein level. Furthermore, small interfering RNA-mediated WWP2 knockdown in liver cancer cell lines was demonstrated to inhibit cell proliferation, cause cell cycle arrested in G1 phase and to induce apoptosis as revealed by a Cell Counting Kit-8 assay and flow cytometric analysis. In addition, western blot analysis revealed that WWP2 knockdown significantly increased the expression of apoptosis-associated markers caspase‑7, caspase‑8 and B-cell lymphoma 2 (Bcl-2)-associated X in liver cancer cell lines, while Bcl‑2 was significantly decreased. In conclusion, the present study suggested that WWP2 may exert important functions in the over‑proliferation and evasion of apoptosis of liver cancer, likely through regulating the expression of apoptosis-associated markers. Furthermore, WWP2 may represent a novel diagnostic marker and molecular therapeutic target for liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xu SQ, Qin Y, Pan DB, Ye GX, Wu CJ, Wang S, Jiang JY, Fu J and Wang CJ: Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells. Mol Med Rep 13: 2261-2266, 2016.
APA
Xu, S., Qin, Y., Pan, D., Ye, G., Wu, C., Wang, S. ... Wang, C. (2016). Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells. Molecular Medicine Reports, 13, 2261-2266. https://doi.org/10.3892/mmr.2016.4771
MLA
Xu, S., Qin, Y., Pan, D., Ye, G., Wu, C., Wang, S., Jiang, J., Fu, J., Wang, C."Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells". Molecular Medicine Reports 13.3 (2016): 2261-2266.
Chicago
Xu, S., Qin, Y., Pan, D., Ye, G., Wu, C., Wang, S., Jiang, J., Fu, J., Wang, C."Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells". Molecular Medicine Reports 13, no. 3 (2016): 2261-2266. https://doi.org/10.3892/mmr.2016.4771
Copy and paste a formatted citation
x
Spandidos Publications style
Xu SQ, Qin Y, Pan DB, Ye GX, Wu CJ, Wang S, Jiang JY, Fu J and Wang CJ: Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells. Mol Med Rep 13: 2261-2266, 2016.
APA
Xu, S., Qin, Y., Pan, D., Ye, G., Wu, C., Wang, S. ... Wang, C. (2016). Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells. Molecular Medicine Reports, 13, 2261-2266. https://doi.org/10.3892/mmr.2016.4771
MLA
Xu, S., Qin, Y., Pan, D., Ye, G., Wu, C., Wang, S., Jiang, J., Fu, J., Wang, C."Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells". Molecular Medicine Reports 13.3 (2016): 2261-2266.
Chicago
Xu, S., Qin, Y., Pan, D., Ye, G., Wu, C., Wang, S., Jiang, J., Fu, J., Wang, C."Inhibition of WWP2 suppresses proliferation, and induces G1 cell cycle arrest and apoptosis in liver cancer cells". Molecular Medicine Reports 13, no. 3 (2016): 2261-2266. https://doi.org/10.3892/mmr.2016.4771
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