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Article Open Access

Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats

  • Authors:
    • Yijing Jiang
    • Shanli Yang
    • Jing Tao
    • Zhicheng Lin
    • Xiaoqian Ye
    • Yongmei You
    • Jun Peng
    • Zhenfeng Hong
    • Lidian Chen
  • View Affiliations / Copyright

    Affiliations: Department of Rehabilitation Medicine, Rehabilitation Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350001, P.R. China, College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2060-2070
    |
    Published online on: January 13, 2016
       https://doi.org/10.3892/mmr.2016.4773
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Abstract

The aim of the present study was to investigate whether the cyclic adenosine 3',5'‑monophosphate (cAMP)/protein kinase A(PKA)/cAMP‑responsive element binding protein (CREB) signal transduction pathway triggered by γ‑aminobutyric acid class B (GABAB) receptor activation is involved in neuroprotection against ischemia and behavioral recovery induced by opposing needling (ON). A total of 80 rats were randomly divided into four groups: A sham operation group, an ischemia group, an ON group and an ON group effectively inhibited by the GABAB receptor antagonist, CGP35384 (n=20/group). The behavior of the rats was assessed by their neurological deficit score, whereas the impairment of gait was examined using the CatWalk system. The volume of cerebral infarction was examined upon treatment with 2,3,5‑triphenyltetrazolium chloride. The expression levels of CREB, GABAB1 and GABAB2 were examined by western blotting and reverse transcription‑quantitative polymerase chain reaction, and the activity of adenylyl cyclase (AC), cAMP and PKA in the serum was detected using an enzyme‑linked immunosorbent assay. In the present study, in comparison with other groups, the ON group exhibited a reduced score for the neurological deficit, the stride length and swing speed were improved, and the volume of infarction was reduced. However, these effects were reversed upon administration of CGP35384. Additionally, the expression levels of CREB, GABAB1 and GABAB2 were increased in the ON group. The levels of AC, cAMP and PKA in the serum were also increased in the ON group, whereas the addition of CGP35384 reversed these effects. The results of the present study demonstrated that ON markedly protected the brain against transient cerebral ischemic injury, and this effect was possibly mediated by the activation of the GABAB/cAMP/PKA/CREB signal transduction pathway. These findings implied that ON may be a potential therapeutic method for treating stroke.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang Y, Yang S, Tao J, Lin Z, Ye X, You Y, Peng J, Hong Z and Chen L: Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats. Mol Med Rep 13: 2060-2070, 2016.
APA
Jiang, Y., Yang, S., Tao, J., Lin, Z., Ye, X., You, Y. ... Chen, L. (2016). Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats. Molecular Medicine Reports, 13, 2060-2070. https://doi.org/10.3892/mmr.2016.4773
MLA
Jiang, Y., Yang, S., Tao, J., Lin, Z., Ye, X., You, Y., Peng, J., Hong, Z., Chen, L."Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats". Molecular Medicine Reports 13.3 (2016): 2060-2070.
Chicago
Jiang, Y., Yang, S., Tao, J., Lin, Z., Ye, X., You, Y., Peng, J., Hong, Z., Chen, L."Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats". Molecular Medicine Reports 13, no. 3 (2016): 2060-2070. https://doi.org/10.3892/mmr.2016.4773
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Y, Yang S, Tao J, Lin Z, Ye X, You Y, Peng J, Hong Z and Chen L: Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats. Mol Med Rep 13: 2060-2070, 2016.
APA
Jiang, Y., Yang, S., Tao, J., Lin, Z., Ye, X., You, Y. ... Chen, L. (2016). Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats. Molecular Medicine Reports, 13, 2060-2070. https://doi.org/10.3892/mmr.2016.4773
MLA
Jiang, Y., Yang, S., Tao, J., Lin, Z., Ye, X., You, Y., Peng, J., Hong, Z., Chen, L."Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats". Molecular Medicine Reports 13.3 (2016): 2060-2070.
Chicago
Jiang, Y., Yang, S., Tao, J., Lin, Z., Ye, X., You, Y., Peng, J., Hong, Z., Chen, L."Opposing needling promotes behavior recovery and exerts neuroprotection via the cAMP/PKA/CREB signal transduction pathway in transient MCAO rats". Molecular Medicine Reports 13, no. 3 (2016): 2060-2070. https://doi.org/10.3892/mmr.2016.4773
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