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Native low density lipoprotein promotes lipid raft formation in macrophages

  • Authors:
    • Jian Song
    • Ling‑Yan Ping
    • Duc M. Duong
    • Xiao‑Yan Gao
    • Chun‑Yan He
    • Lei Wei
    • Jun‑Zhu Wu
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA, Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China
    Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2087-2093
    |
    Published online on: January 14, 2016
       https://doi.org/10.3892/mmr.2016.4781
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Abstract

Oxidized low‑density lipoprotein (LDL) has an important role in atherogenesis; however, the mechanisms underlying cell‑mediated LDL oxidation remain to be elucidated. The present study investigated whether native‑LDL induced lipid raft formation, in order to gain further insight into LDL oxidation. Confocal microscopic analysis revealed that lipid rafts were aggregated or clustered in the membrane, which were colocalized with myeloperoxidase (MPO) upon native LDL stimulation; however, in the presence of methyl‑β‑cyclodextrin (MβCD), LDL‑stimulated aggregation, translocation, and colocalization of lipid rafts components was abolished.. In addition, lipid raft disruptors MβCD and filipin decreased malondialdehyde expression levels. Density gradient centrifugation coupled to label‑free quantitative proteomic analysis identified 1,449 individual proteins, of which 203 were significantly upregulated following native‑LDL stimulation. Functional classification of the proteins identified in the lipid rafts revealed that the expression levels of translocation proteins were upregulated. In conclusion, the results of the present study indicated that native‑LDL induced lipid raft clustering in macrophages, and the expression levels of several proteins were altered in the stimulated macrophages, which provided novel insights into the mechanism underlying LDL oxidation.
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Copy and paste a formatted citation
Spandidos Publications style
Song J, Ping LY, Duong DM, Gao XY, He CY, Wei L and Wu JZ: Native low density lipoprotein promotes lipid raft formation in macrophages. Mol Med Rep 13: 2087-2093, 2016.
APA
Song, J., Ping, L., Duong, D.M., Gao, X., He, C., Wei, L., & Wu, J. (2016). Native low density lipoprotein promotes lipid raft formation in macrophages. Molecular Medicine Reports, 13, 2087-2093. https://doi.org/10.3892/mmr.2016.4781
MLA
Song, J., Ping, L., Duong, D. M., Gao, X., He, C., Wei, L., Wu, J."Native low density lipoprotein promotes lipid raft formation in macrophages". Molecular Medicine Reports 13.3 (2016): 2087-2093.
Chicago
Song, J., Ping, L., Duong, D. M., Gao, X., He, C., Wei, L., Wu, J."Native low density lipoprotein promotes lipid raft formation in macrophages". Molecular Medicine Reports 13, no. 3 (2016): 2087-2093. https://doi.org/10.3892/mmr.2016.4781
Copy and paste a formatted citation
x
Spandidos Publications style
Song J, Ping LY, Duong DM, Gao XY, He CY, Wei L and Wu JZ: Native low density lipoprotein promotes lipid raft formation in macrophages. Mol Med Rep 13: 2087-2093, 2016.
APA
Song, J., Ping, L., Duong, D.M., Gao, X., He, C., Wei, L., & Wu, J. (2016). Native low density lipoprotein promotes lipid raft formation in macrophages. Molecular Medicine Reports, 13, 2087-2093. https://doi.org/10.3892/mmr.2016.4781
MLA
Song, J., Ping, L., Duong, D. M., Gao, X., He, C., Wei, L., Wu, J."Native low density lipoprotein promotes lipid raft formation in macrophages". Molecular Medicine Reports 13.3 (2016): 2087-2093.
Chicago
Song, J., Ping, L., Duong, D. M., Gao, X., He, C., Wei, L., Wu, J."Native low density lipoprotein promotes lipid raft formation in macrophages". Molecular Medicine Reports 13, no. 3 (2016): 2087-2093. https://doi.org/10.3892/mmr.2016.4781
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